The following antibodies were used for immunoblot analysis: ZAK TV-1 (Bethyl; A301-993A), ZAK TV-1 and TV-2 (Sigma; HPA017205), FLAG (Sigma; A8592), Tubulin (Sigma; T6074), and horseradish peroxidase-conjugated anti-mouse and anti-rabbit secondary antibodies (Jackson ImmunoResearch). The Sigma HPA017205 antibody also recognizes additional bands when probing for endogenous levels of ZAK, some of which run at the same molecular weight as ZAK TV-1. Therefore, the Bethyl A301-993A antibody was used for probing for endogenous ZAK TV-1.
Liu J., McCleland M., Stawiski E.W., Gnad F., Mayba O., Haverty P.M., Durinck S., Chen Y.J., Klijn C., Jhunjhunwala S., Lawrence M., Liu H., Wan Y., Chopra V., Yaylaoglu M.B., Yuan W., Ha C., Gilbert H.N., Reeder J., Pau G., Stinson J., Stern H.M., Manning G., Wu T.D., Neve R.M., de Sauvage F.J., Modrusan Z., Seshagiri S., Firestein R, & Zhang Z. (2014). Integrated exome and transcriptome sequencing reveals ZAK isoform usage in gastric cancer. Nature Communications, 5, 3830.
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