Total RNA was isolated from the exosomes of early-stage NPC plasma and normal plasma using TRlzol reagent (Invitrogen, South San Francisco, CA, USA) and treated with RNase-free DNase I to remove genomic DNA. RNA degradation and contamination were assessed on 1% agarose gels. The purity, concentration, and integrity of the RNAs were checked using the NanoPhotometer® spectrophotometer (Implen, CA, USA), Qubit®. RNA Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, CA, USA),degradation and RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). All procedures were conducted according to the manufacturer’s instructions.
Rna assay kit in qubit 2.0 fluorometer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The RNA Assay Kit in Qubit® 2.0 Fluorometer is a quantification tool designed to accurately measure RNA concentrations. It utilizes fluorescent dyes that bind specifically to RNA, allowing for precise quantification of RNA samples.
Automatically generated - may contain errors
2 protocols using rna assay kit in qubit 2.0 fluorometer
1
Exosomal RNA Isolation and Characterization
2
Exosomal RNA Isolation from NPC Plasma
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Total RNA was isolated from the exosomes of early-stage NPC plasma and normal plasma using TRlzol reagent (Invitrogen, South San Francisco, CA, USA) and treated with RNase-free DNase I to remove genomic DNA. RNA degradation and contamination were assessed on 1% agarose gels. The purity, concentration, and integrity of the RNAs were checked by the NanoPhotometer® spectrophotometer (Implen, CA, USA), Qubit®. RNA Assay Kit in Qubit® 2.0 Fluorometer (Life Technologies, CA, USA),degradation and RNA Nano 6000 Assay Kit with the Agilent Bioanalyzer 2100 system (Agilent Technologies, CA, USA). All procedures were conducted under the manufacturer's instructions.
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