Given that atrophy of the corpus callosum is a common pathologic feature in CBD [62 (link)], the thickness of the corpus callosum was measured as previously described [33 (link)]. Hematoxylin and eosin stained sections of the anterior cingulate gyrus, obtained from coronal brain slices at the level of nucleus accumbens, were scanned on the ScanScopeXT (Aperio Technologies, Vista, CA). The ImageScope-11.2 (Aperio Technologies) ruler feature was used to measure the thickness at a consistent location in all cases as shown in Supplementary Figure 1 (Online Resource 2)
To quantify the tau burden, digital image analysis was performed in select brain regions [28 (link)]. CP13-stained sections of the pons, medulla, and cerebellar hemisphere were scanned on the ScanScopeXT. The pontine base, inferior olivary nucleus, and cerebellar white matter were annotated using ImageScope-11.2 and analyzed in Spectrum-11.2 (Aperio Technologies) using a custom-designed color deconvolution algorithm to detect only CP13-positive pathology as shown inSupplementary Figure 2 (Online Resource 3)
To quantify the tau burden, digital image analysis was performed in select brain regions [28 (link)]. CP13-stained sections of the pons, medulla, and cerebellar hemisphere were scanned on the ScanScopeXT. The pontine base, inferior olivary nucleus, and cerebellar white matter were annotated using ImageScope-11.2 and analyzed in Spectrum-11.2 (Aperio Technologies) using a custom-designed color deconvolution algorithm to detect only CP13-positive pathology as shown in