Celigo imaging cytometry system version 2

Cell migration was evaluated via wound healing experiment. Lentivirus-transfected A549 and NCI-H1299 cells were seeded in 96-well plates at a density greater than 90% confluency. After 24 h of culture, scratches were formed by a scratch tester, and the old medium was replaced with a medium containing 1% FBS. The scratches were monitored at 0, 24 and 48 h. The migration area was analyzed using Celigo Imaging Cytometry System version 2.0 (Nexcelom Bioscience, Lawrence, MA, USA). Each experiment was repeated three times.

A549 cells and NCI-H1299 cells in the logarithmic growth phase were selected. The fluorescence of these cells was detected using the Celigo Imaging Cytometry System version 2.0 (Nexcelom Bioscience, Lawrence, MA, USA) and the number of cells was automatically calculated. MTT assay kit (Genview, Houston, TX, USA) was used to detect cell proliferation. According to the manufacturer’s instructions, the cells were inoculated into 96-well plates at a density of 1500 cells/well, cultured continuously and analyzed for 5 days, following treatment with MTT for 4 h. Subsequently, the optical density (OD) was measured at 490 nm using a microplate reader (Tecan Infinite, Männedorf, Switzerland). Each experiment was repeated three times.