Icr mice

Manufactured by Japan SLC

Sourced in Japan

ICR mice are a breed of laboratory mice commonly used in scientific research. They are a stock of outbred mice that exhibit genetic diversity, making them a useful model for various studies. The ICR mice are maintained by Japan SLC, a leading provider of high-quality laboratory animals.

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Lab products found in correlation

B6d2f1, by Japan SLC (5 mentions) C57bl 6j mice, by Japan SLC (5 mentions) C57bl 6j, by Japan SLC (4 mentions) Trypsin edta, by Thermo Fisher Scientific (3 mentions) Sox9 egfp mice, by RIKEN BioResource Center (2 mentions) C57bl 6j mice, by Charles River Laboratories (2 mentions) C57bl 6n mice, by Japan SLC (2 mentions) Dextran sulfate sodium (dss), by MP Biomedicals (2 mentions) C57bl 6 mice, by Japan SLC (2 mentions) C57bl 6n, by Japan SLC (2 mentions) Neurobasal medium, by Thermo Fisher Scientific (2 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions) B27 supplement, by Thermo Fisher Scientific (2 mentions) Micro bicinchoninic acid bca protein assay kit, by Thermo Fisher Scientific (1 mentions) Dynabead, by Thermo Fisher Scientific (1 mentions) Infinite 200, by Tecan (1 mentions) Dba 2, by Japan SLC (1 mentions) Samp8 mice, by Japan SLC (1 mentions) U0126, by Cell Signaling Technology (1 mentions) Penicillin streptomycin, by Thermo Fisher Scientific (1 mentions)

143 protocols using icr mice

Isolation and Purification of Murine Germ Cells

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PGCs and gonocytes were collected from pregnant ICR mice (Japan SLC, Shizuoka, Japan) that were maintained in a controlled environment with 12:12-h light:dark cycles (lights on from 0800 to 2000 h). The day on which a copulation plug was found was designated as 0.5 dpc. The sex of the embryos was determined using a polymerase chain reaction (PCR)-based genotyping method and the Ube1 gene [16 (link)], and only male embryos were used in the current study. Cells were collected from germ cell-containing regions by dissection of the posterior thirds of 8.5 dpc embryos, the mesentery and urogenital sinuses of 10.5 dpc embryos and the genital ridges of 12.5, 15.5 and 18.5 dpc embryos. Tissues were digested in 0.25% trypsin/1 mM EDTA for 10 min. For collection of pup testis cells, we used 8- to 10-day-old ICR mice (Japan SLC). Testis cells were dissociated using a two-step enzymatic digestion protocol with collagenase type IV and
trypsin, as described previously [17 (link)]. Dissociated testis cells were then incubated with biotin-conjugated anti-CD9 antibody (MZ3; BioLegend, San Diego, CA, USA) for 10 min at 4 C. Magnetic cell sorting (MACS) was then performed using streptavidin-conjugated Dynabeads according to the manufacturer’s instructions (Invitrogen, Carlsbad, CA, USA).

TANAKA T., KANATSU-SHINOHARA M., HIROSE M., OGURA A, & SHINOHARA T. (2015). Pluripotent cell derivation from male germline cells by suppression of Dmrt1 and Trp53. The Journal of Reproduction and Development, 61(5), 473-484.

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Mouse Gamete Collection and Embryo Transfer Protocol

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The C57BL/6J (B6) and ICR mice were purchased from Japan SLC while the SJL/J mice [JAX
Mice Strain SJL/J (Stock Number 000686)] were from Charles River Laboratories Japan. These
mice were used as donors for sperm (from mice 12–15 weeks of age) and oocytes (from B6
mice 4 weeks of age and ICR and SJL mice 8 weeks of age). ICR mice (8–16 weeks of age;
Japan SLC) were used as recipients of 2-cell embryos. All animals were maintained in a
specific pathogen-free space under a 12-h light/dark regimen. Experimental procedures were
performed in accordance with the Guide for the Care and Use of Laboratory Animals of the
Science Council of Japan and were approved by the Animal Experiment Committee of Gunma
University.

Kaneko R., Kakinuma T., Sato S., Jinno-Oue A, & Hata H. (2014). Littermate Influence on Infant Growth in Mice: Comparison of SJL/J and ICR as Cotransferred Carrier Embryos. Experimental Animals, 63(4), 375-381.

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Ovarian Tissue Transplantation in Mice

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Day 3, 10, and 21, and 30 and 50 week old female ICR mice (CLEA Japan and Japan SLC, Tokyo, Japan) were housed under a 12 hours light/dark cycle at 22 °C and fed ad libitum for ovarian tissue harvest. Under these housing conditions, 6 weeks old female ICR mice (Japan SLC, Tokyo, Japan) acted as ovarian transplantation recipients, and 10 weeks old female ICR mice acted as host mothers (Japan SLC, Tokyo, Japan). The experimental protocols and animal-handling procedures were performed with the approval of the Institutional Animal Care and Use Committee (IACUC) of St. Marianna University School of Medicine. Dissection, ovarian tissue transplantation, and in vitro fertilization were performed according to IACUC approved protocols.

Takae S., Tsukada K., Sato Y., Okamoto N., Kawahara T, & Suzuki N. (2017). Accuracy and safety verification of ovarian reserve assessment technique for ovarian tissue transplantation using optical coherence tomography in mice ovary. Scientific Reports, 7, 43550.

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Intrauterine Electroporation of Pregnant Mice

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Pregnant ICR mice were purchased from Japan SLC, Inc. (Hamamatsu, Japan). The day of vaginal plug observation was designated as E0.5. E13.5 to E15.5 pregnant mice were used for IUE. All animal experiments were performed under the control of the Institutional Animal Care and Use Committee of Waseda University. The experimental protocols for the animal experiments was approved by the Committee on the Ethics of Animal Experiments of Waseda University.

Li B., Chavarha M., Kobayashi Y., Yoshinaga S., Nakajima K., Lin M.Z., & Inoue T. (2020). Two-photon voltage imaging of spontaneous activity from multiple neurons reveals network activity in brain tissue.

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Animal Welfare Approved Murine Study

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B6D2F1 and ICR mice were purchased from Japan SLC Inc. All animal studies were approved by the Animal Experiments Committee of Fukushima Medical University, Japan, and performed under the guidelines and regulations of Fukushima Medical University.

Saito T., Wada I, & Inoue N. (2019). Alternative splicing of the Izumo1 gene ensures triggering gamete fusion in mice. Scientific Reports, 9, 3151.

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Tissue Fractionation and Protein Quantification

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ICR mice were purchased from Japan SLC (Shizuoka, Japan) and raised in our laboratory. Cytosol and membrane extracts of various tissues were prepared from postnatal day 40 (P40) mice of either sex as described previously [7 (link)]. Briefly, tissues were homogenized with 10 vol. of 50 mM Tris/pH 7.5 containing 0.1 M NaF, 5 mM EDTA, 1 mM Na₃VO₄, 10 μg/ml aprotinin and 10 μg/ml leupeptin (buffer S). Each suspension was sonicated at 0°C for 1 min and centrifuged at 125,000 g for 20 min at 4°C. The supernatants were used as cytosolic extracts and the pellets were washed once by sonication and centrifugation with buffer S and then solubilized with buffer S containing 2% SDS. In some experiments, brain tissues were directly homogenized with buffer S containing 2% SDS to obtain the whole tissue extracts [6 (link)]. Protein concentration was estimated with a micro bicinchoninic acid (BCA) protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA) with bovine serum albumin (BSA) as a standard. Western blotting was done as described earlier.

Ibaraki K., Mizuno M., Aoki H., Niwa A., Iwamoto I., Hara A., Tabata H., Ito H, & Nagata K.I. (2018). Biochemical and Morphological Characterization of a Guanine Nucleotide Exchange Factor ARHGEF9 in Mouse Tissues. Acta Histochemica et Cytochemica, 51(3), 119-128.

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FRET Imaging of ERK Biosensor Mice

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For FRET imaging, we used transgenic mice that ubiquitously express an ERK biosensor with a long flexible linker, which has been described elsewhere (Harvey et al., 2008; (link)Komatsu et al., 2011 (link)Komatsu et al., , 2018)) (link). Sox9-EGFP mice were provided from RIKEN BRC through the National BioResource Project of the MEXT/AMED, Japan (RBRC05651). Otherwise, we used ICR mice purchased from Japan SLC, Inc. The midnight preceding observation of a plug was designated as embryonic day 0.0 (E0.0), and all mice were sacrificed by cervical dislocation to minimize suffering. All the animal experiments were approved by the local ethical committee for animal experimentation (MedKyo 19090 and 20081) and were performed in compliance with the guide for the care and use of laboratory animals at Kyoto University.

Yoshida T., Matsuda M., & Hirashima T. (2020). Incoherent feedforward regulation via Sox9 and Erk underpins mouse tracheal cartilage development.

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Hemolysis Assay of Red Blood Cells

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Whole blood of ICR mice was collected from inferior vena cava in the presence of 0.5 μL of heparin sodium (1000 U/mL). The ICR mice (male, 6–7 weeks) were purchased from Japan SLC, Inc. (Shizuoka, Japan). The experimental protocols were reviewed and approved by the Chiba University Animal Care Committee in accordance with the “Guide for Care and Use of Laboratory Animals”. The ethical approval code issued from the committee was 30-41. Red blood cells were purified by washing the blood (1 mL) with 9 mL of PBS. The blood was centrifuged (4 °C, 400× g, 5 min) and the supernatant was discarded by aspiration. The washing was repeated 5 times so as to completely remove serum proteins. PMBS buffer was prepared by dissolving DL-malic acid by PBS(-). Final concentration of the malic acid was 20 mM. The pH was then adjusted by NaOH solution. The red blood cells were then incubated with the LNP at pH from 5.5 to 7.4. The final concentration of the total lipid was 100 µM. The mixture was incubated at 37 °C for 30 min. After the incubation, the samples were centrifuged at 500× g for 5 min. The absorbance of the leaked hemoglobin in supernatant was measured at 545 nm by plate reader Infinite 200 (Tecan Japan Co., Ltd., Kanagawa, Japan). As a positive control, the red blood cells were lysed by 0.1% (wt/v) Triton-X100.

Tanaka H., Miyama R., Sakurai Y., Tamagawa S., Nakai Y., Tange K., Yoshioka H, & Akita H. (2021). Improvement of mRNA Delivery Efficiency to a T Cell Line by Modulating PEG-Lipid Content and Phospholipid Components of Lipid Nanoparticles. Pharmaceutics, 13(12), 2097.

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Generation and Use of Pcdh10 Knockout Mice

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Pregnant ICR mice were purchased from Japan SLC (Hamamatsu, Japan). The Pcdh10 (OL-pc) knockout mouse line generated by Lexicon Pharmaceuticals (The Woodlands, TX) has been described previously (Uemura et al., 2007 (link)). The morning of vaginal plug detection was designated as E0.5. All animal experiments were performed under the control of the Keio University Institutional Animal Care and Use Committee in accordance with Institutional Guidelines on Animal Experimentation at Keio University.

Oishi K., Nakagawa N., Tachikawa K., Sasaki S., Aramaki M., Hirano S., Yamamoto N., Yoshimura Y, & Nakajima K. (2016). Identity of neocortical layer 4 neurons is specified through correct positioning into the cortex. eLife, 5, e10907.

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Maintenance of Specific Pathogen-Free Mice

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Male DBA/2 and ICR mice were purchased from Japan SLC. Mice were maintained under specific pathogen-free (SPF) conditions at 23 ± 1ºC with a constant humidity of 55 ± 5% under a 12-h light/dark cycle, and had free access to food (CE-2) and tap water in accordance with the Guidelines for Experimental Animal Care issued by the Prime Minister' s Office of Japan. Mice were used in the present study at 5 weeks of age. All animal experiments followed the guidelines for laboratory animal experiments by Tokyo University of Pharmacy and Life Sciences (TUPLS), and each of the experimental protocols was approved by the Committee of Laboratory Animal Experiments in TUPLS (P15-42).

Tanaka H., Yanai C., Miura N.N., Ishibashi K.I., Yamanaka D., Ohnishi H., Ohno N, & Adachi Y. (2020). Coronary Vasculitis Induced in Mice by Cell Wall Mannoprotein Fractions of Clinically Isolated Candida Species. Medical mycology journal, 61(3).

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