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Peptide and protein calibration standards

Manufactured by Bruker
Sourced in Germany

Peptide and protein calibration standards are reference materials used to calibrate and validate the performance of analytical instruments in the measurement and identification of peptides and proteins. These standards provide known and traceable concentrations or molecular weights of specific peptide and protein analytes to ensure accurate and reliable results in proteomics and related applications.

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2 protocols using peptide and protein calibration standards

1

Proteomic Sample Preparation Protocol

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The reagents and devices such as C18 Nu tips (Glysci.com), Spectra/Por membranes (Spectrumlabs.com), bicinchoninic acid (BCA) protein assay kit, Pierce C18 spin columns, MS grade trypsin (Fisher Scientific.com), peptide and protein calibration standards (m/z 500–16000, Bruker Daltonics, Bremen, Germany), and 2-iodoacetamide (IAA) (MP Biomedical, OH) were purchased from their respective vendors. Most other chemicals and supplies including 1, 4-dithiothreitol (DTT), 2, 5-dihydroxybenzoic acid (DHB), were purchased from Sigma Aldrich (St. Louis, MO).
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2

MALDI-TOF Mass Spectrometry Calibration

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Sinapinic acid (SA), 2,5-dihydroxybenzoic acid (DHB) and all solvents (HPLC grade) used were purchased from Sigma-Aldrich (MO, USA), unless otherwise noted. Conductive indium-tin oxide (ITO) one-side coated glass slides and peptide and protein calibration standards were purchased from Bruker Daltonik GmbH (Germany).
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