Gi me n

All cell lines (RH4, RH30, RD, T174, TE381.1, HD-MB03, DAOY, ONS76, UW228, IMR5, GI-ME-N, NBL-S, Kelly, LAN-1, and CHP-212) were obtained at American Type Culture Collection (ATCC, Virginia, US) if not otherwise specified. Rh41, Rh18, Rh36, TE441, and Kym1 were kindly provided by Prof. Simone Fulda. Cell lines were cultured under standard conditions in Roswell Park Memorial Institute medium 1640 (RPMI1640) or Dulbecco's Modified Eagle Medium (DMEM) supplemented with 10% Fetal Calf Serum (FCS, Sigma–Aldrich, Missouri, USA) and 1% Penicillin/Streptomycin (Thermo Fisher Scientific). The identity of all cell lines was verified by STR genotyping (Genetica DNA Laboratories), and cells were periodically checked with Lonza MycoAlert system.

The human neuroblastoma cell lines, IMR32, SKNFI, SKNAS, LAN-5, GIMEN, were obtained from the American Type Culture Collection. All cells were maintained in DMEM (Dulbecco’s Modified Eagle Medium, Thermo Fisher Scientific) supplemented with 10% fetal calf serum, 100 U/mL penicillin-streptomycin (Pen/Strep, Gibco, life technologies), and 2% MEM non-essential amino acids solution (Thermo Fisher Scientific), hereafter called complete DMEM. All cell lines were grown at 37°C in a humidified incubator containing 5% CO₂. ExpiCHO-S cells (Thermo Fisher Scientific) were cultured in ExpiCHO Expression Medium (Gibco) at 37°C, 8% CO2, shaking at 125 rpm. Cells were not cultured past 20 passages, and they were regularly tested for mycoplasma contamination by a Mycoalert mycoplasma detection kit (Lonza).