Anti map1lc3 lc3

Manufactured by Cell Signaling Technology

Anti-MAP1LC3/LC3 is a primary antibody that recognizes the microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B), also known as LC3B. LC3B is a widely used autophagosome marker that is involved in the formation and maturation of autophagosomes.

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Close spelling variants of this product

Anti-LC3 (225 citations) MAP1LC3 (3 citations)

2 protocols using anti map1lc3 lc3

Comprehensive Antibody Collection for Protein Analysis

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The following antibodies were obtained from Cell Signaling Technology (Beverly, MA): anti-pan-acetylated lysine (#9441), anti-MAP1LC3/LC3 (#4108), anti-RPS6KB/p70S6K (#9202), anti-phospho-RPS6KB/p70S6K (Thr389) (#9205), anti-TSC2 (#9442), anti-phospho-ULK1 (Ser 757) (#14202), anti-ULK1 (#8054), anti-phospho-DNM1L/Drp1 (Ser 616) (#3455), anti-phospho-MAPK3/ERK1-MAPK1/ERK2 (Thr 202/Tyr 204) (#9101), anti-MAPK3/ERK1-MAPK1/ERK2 (#9102), and anti-P-EIF2A/P-eIF2-α (Ser 51) (#9721). From Mitosciences: anti-RIESKE (MS305/C0183). From Abcam: anti-MFN2 (ab56889) and anti-HADHA (ab54477). From Sigma-Aldrich: anti-ACTB/β-actin (A5316). From Santa Cruz Biotechnology: anti-GFP (sc-9996), anti-TSC2 (sc-893), anti-TOMM20 (sc-17764), and anti-MFN1 (sc-50330). From Merck-Millipore: Nitrotyrosine 06-284. Other antibodies were used as follows: anti-mono- and polyubiquitinylated proteins FK2 conjugated with peroxidase (HRP) or FK2H from Enzo Life Sciences (BML-PW0150), anti-OPA1 (612606), and anti-DNM1L/Drp1 (611112) from BD Biosciences. Chloroquine C6628, propidium iodide (P4170), and thapsigargin (T9033) were from Sigma-Aldrich; rapamycin (553210) and resveratrol (R5010) were from Merck; and geneticin (G418) was from Santa Cruz (sc-29065).

Hernández M.G., Aguilar A.G., Burillo J., Oca R.G., Manca M.A., Novials A., Alcarraz-Vizan G., Guillén C, & Benito M. (2018). Pancreatic β cells overexpressing hIAPP impaired mitophagy and unbalanced mitochondrial dynamics. Cell Death & Disease, 9(5), 481.

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Antibody Optimization for Cell Signaling

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The following antibodies were obtained from Cell Signaling Technology (Beverly, MA): anti-MAP1LC3/LC3 (#4108), anti-RPS6KB/p70S6K (#9202), anti-phospho-RPS6KB/p70S6K (Thr389) (#9205), anti-TSC2 (D93F12, #4308), anti-phospho-RAPTOR (Ser 792) (#2083), anti-RAPTOR (#2280), anti-cleaved caspase-3 (#9661), anti-phospho-ACC (Ser 79) (#3661). Anti-phospho-S6 was obtained from Thermo-Scientific (MA5-15140). Anti-LAMP2 (#ab13524) antibody was obtained from Abcam. The anti-glucokinase (GK) (sc-7908) was obtained from Santa Cruz. From Sigma-Aldrich: anti-ACTB/β-actin (A5316). Chloroquine C6628 and propidium iodide (P4170) were from Sigma-Aldrich; 2-deoxy-glucose (2DG) (D8375) was from Merck; and geneticin (G418) (sc-29065) and hygromycin (sc-29067) were from Santa Cruz.

Marqués P., Kamitz A., Bartolomé A., Burillo J., Martínez H., Jiménez B., Fernández-Rhodes M., Guillén C, & Benito M. (2019). Essential role of glucokinase in the protection of pancreatic β cells to the glucose energetic status. Cell Death Discovery, 5, 138.

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