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Ix 70 microscope

Manufactured by Cytiva
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Sourced in United States

The IX-70 microscope is a high-quality laboratory instrument designed for a variety of microscopy applications. It features a sturdy and ergonomic construction, providing a stable platform for precise observations. The IX-70 incorporates advanced optical components to deliver clear, high-resolution images across a range of magnification levels. Its functionality is tailored to meet the needs of researchers and scientists working in diverse fields of study.

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Lab products found in correlation

Deltavision rt, by Cytiva (3 mentions) Uplans apo 100x objective lens, by Olympus (3 mentions) 4 6 diamidino 2 phenylindole (dapi), by Thermo Fisher Scientific (3 mentions) Nanomover and softworx deltavision image acquisition software, by Cytiva (2 mentions) Goat anti mouse igg conjugated with alexa fluor 568, by Thermo Fisher Scientific (1 mentions) Softworx software, by Cytiva (1 mentions) A1r confocal laser microscope system, by Nikon (1 mentions) Delta vision imaging system, by Cytiva (1 mentions) Elvanol, by DuPont (1 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Polyclonal anti fibronectin, by Merck Group (1 mentions) Alexa 647 conjugated goat anti rabbit antibody, by Thermo Fisher Scientific (1 mentions) Softworx, by Cytiva (1 mentions) Softworx, by GE Healthcare (1 mentions)

Close spelling variants of this product

IX-70 inverted microscope

7 protocols using ix 70 microscope

1

Immunofluorescence Staining of Influenza A

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Cells were fixed with 4% paraformaldehyde in PBS for 20 min at room temperature and washed with PBS. Cells were permeabilized with 0.1% Triton X-100 in PBS at room temperature for 5 min. After blocking with 3% non-fat dry milk for 30 min, cells were incubated with mouse monoclonal anti-influenza A nucleoprotein (AbD Serotec); bound antibodies were revealed with goat anti-mouse IgG conjugated with Alexa Fluor 568 (Molecular Probes). After washing, nuclei were stained with 1 μg ml−1 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes) in PBS for 15 min at room temperature. Fluorescent images were acquired on an Olympus IX70 microscope equipped with Nanomover and softWoRx DeltaVision image acquisition software (Applied Precision, WA, USA) and a U-PLAN-APO 60× objective. Images were captured under constant exposure time, gain and offset. To improve the contrast and resolution of digital images captured in the microscope, they were elaborated with deconvolution software.
Amatore D., Sgarbanti R., Aquilano K., Baldelli S., Limongi D., Civitelli L., Nencioni L., Garaci E., Ciriolo M.R, & Palamara A.T. (2014). Influenza virus replication in lung epithelial cells depends on redox-sensitive pathways activated by NOX4-derived ROS. Cellular Microbiology, 17(1), 131-145.
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2

Deconvolved Microscopy Image Capture

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Images were collected in a z stack using an Olympus IX70 microscope and Softworx software (Applied Precision). The final images shown are maximum-intensity projections of deconvolved z stacks.
Lee C.S., Bhaduri A., Mah A., Johnson W.L., Ungewickell A., Aros C.J., Nguyen C.B., Rios E.J., Siprashvili Z., Straight A., Kim J., Aasi S.Z, & Khavari P.A. (2014). Recurrent point mutations in the kinetochore gene KNSTRN in cutaneous squamous cell carcinoma. Nature genetics, 46(10), 1060-1062.
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3

Immunofluorescence Staining of Cultured Cells and Tissue

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The cultured cells on coverslips were fixed in 4% formaldehyde, permeabilized by incubation in 0.5% Triton X-100, blocked with 10% goat serum, and then stained with polyclonal anti-fibronectin (Sigma-Aldrich) followed by Alexa-647 conjugated goat anti-rabbit antibody (Molecular Probes/Invitrogen). Afterwards, the samples were stained for f-actin, g-actin by Alexa Fluor® 488 phalloidin and the nuclei by DAPI (Molecular Probes/Invitrogen). To assess the presence of platelets in the colonic mucosa, sectioned colonic tissue mounted on the slides were stained with a rat anti-CD41 antibody, followed by a FITC conjugated goat anti-rat polyclonal antibody, and the nuclei by DAPI, based upon a previously described technique (31 (link), 32 (link)).
Finally, samples were mounted on slides with elvanol (DuPont). Specimens were then visualized either by Deconvolution Microscopy employing an Olympus IX-70 microscope connected to a DeltaVision imaging system (Applied Precision, Issaquah WA), or Nikon A1R confocal laser microscope system and the NIS-Elements C software. The quantitation of immunofluorescence density was done using ImageJ software by measuring pixel units.
Lichtenberger L.M., Fang D., Bick R.J., Poindexter B.J., Phan T., Bergeron A.L., Pradhan S., Dial E.J, & Vijayan K.V. (2016). Unlocking aspirin’s chemopreventive activity: Role of irreversibly inhibiting platelet cyclooxygenase-1. Cancer prevention research (Philadelphia, Pa.), 10(2), 142-152.
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4

High-Resolution Cellular Imaging

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Immunofluorescence images were taken using an Olympus UPlanS APO 100X objective lens (numerical aperture 1.40) on an Olympus IX-70 microscope equipped with the DeltaVision RT (Applied Precision) imaging system. Z-section image series were collected at 0.2 μM intervals over a total of 3–4 μM through the center of the cells and deconvolved using softWoRx (Applied Precision).
Yellman C.M, & Roeder G.S. (2015). Cdc14 Early Anaphase Release, FEAR, Is Limited to the Nucleus and Dispensable for Efficient Mitotic Exit. PLoS ONE, 10(6), e0128604.
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5

Immunofluorescent Detection of HCV Core

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Cells were fixed in methanol : acetone (50 : 50, V/V) for 20 min at -20°C. After blocking with PBS containing 1% bovine serum albumin (BSA) for at least 30 min, cells were incubated with mouse monoclonal antihepatitis C virus core 1b antibody (Abcam, AB 58713); bound antibodies were revealed with mouse anti-human IgG conjugated with Alexa Fluor 488 (Life Technologies, Z25102). After washing, the nuclei were stained with 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI; Molecular Probes) in PBS for 15 min at room temperature. Fluorescent images were acquired on an Olympus IX70 microscope equipped with Nanomover and softWoRx DeltaVision image acquisition software (Applied Precision, WA, USA) and a U-PLAN-APO 40× objective. Images were captured under constant exposure time, gain, and offset.
Anticoli S., Amatore D., Matarrese P., De Angelis M., Palamara A.T., Nencioni L, & Ruggieri A. (2019). Counteraction of HCV-Induced Oxidative Stress Concurs to Establish Chronic Infection in Liver Cell Cultures. Oxidative Medicine and Cellular Longevity, 2019, 6452390.
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6

Deconvolution of Microscopy Images

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Images were taken using an Olympus UPlanS APO 100X objective lens (numerical aperture 1.40) on an Olympus IX-70 microscope equipped with the DeltaVision RT (Applied Precision, GE Healthcare) imaging system. Z-section image series were collected at 0.2 μM intervals over a total of 3–4 μM through the center of the cells. All protein and DNA localization was done with images that had been deconvolved using SoftWoRx (Applied Precision, GE Healthcare).
The images were converted into RGB.tif files using the image processing software Fiji. To reduce background, the red, green and blue channel levels were all set to a minimum level of 25 on a scale from 1 to 255 using Photoshop.
, & Yellman C.M. (2023). Saccharomyces cerevisiae deficient in the early anaphase release of Cdc14 can traverse anaphase I without ribosomal DNA disjunction and successfully complete meiosis. Biology Open, 12(10), bio059853.
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7

High-resolution Fluorescence Microscopy Protocol

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Images were taken using an Olympus UPlanS APO 100X objective lens (numerical aperture 1.40) on an Olympus IX-70 microscope equipped with the DeltaVision RT (Applied Precision, GE Healthcare) imaging system. Z-section image series were collected at 0.2 μM intervals over a total of 3-4 μM through the center of the cells. All protein and DNA localization was done with images that had been deconvolved using SoftWoRx (Applied Precision, GE Healthcare).
The images were converted into RGB.tif files using the image processing software Fiji. To reduce background, the red, green and blue channel levels were all set to a minimum level of 25 on a scale from 1 to 255 using Photoshop.
Yellman C.M. (2021). Saccharomyces cerevisiaedeficient in the early anaphase release of Cdc14 can traverse anaphase I without ribosomal DNA disjunction and successfully complete meiosis.
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