Sox2 sc17319

Manufactured by Santa Cruz Biotechnology

Sourced in United States

SOX2 (sc17319) is a primary antibody produced by Santa Cruz Biotechnology. It is designed to detect SOX2 protein expression. SOX2 is a transcription factor that plays a critical role in the maintenance of embryonic and adult stem cell pluripotency.

Automatically generated - may contain errors

Lab products found in correlation

2 protocols using sox2 sc17319

Antibody Sources and Reagents for EMT Study

Check if the same lab product or an alternative is used in the 5 most similar protocols

Polyclonal antibody to CD44 (ab41478) was obtained from Abcam (Seoul, Korea). Polyclonal antibodies to AKT (9272S), p-AKT (S473) (9271S), p-AKT (T308) (9275S), SNAIL (3879), P38 (9121), and p-p38 (9211S) were obtained from Cell Signaling Technology (Beverly, MA, USA). Polyclonal antibodies to vimentin (sc-5565), SLUG (SC-10436), TWIST (sc-15393), c-SRC (sc-19), Lyn (sc-15), Fyn (sc-16), LCK (sc-13), and SOX2 (sc17319) were purchased from Santa Cruz Biotechnology (Santa Cruz, CA, USA). Polyclonal antibody to ZEB1 (HPA027524) was purchased from Sigma (St. Louis, MO, USA). Polyclonal antibodies to N-cadherin (610920) and E-cadherin (612130) were purchased from BD Transduction Laboratory (Seoul, Korea). Monoclonal antibodies to β-actin and DAPI were purchased from Sigma. Etoposide (341205), cisplatin (232120), and LY294002 a chemical inhibitor specific to PI3K (440202), were purchased from Calbiochem (San Diego, CA, USA). Paclitaxel (T1912-5MG) was purchased from Sigma.

Kim R.K., Cui Y.H., Yoo K.C., Kim I.G., Lee M., Choi Y.H., Suh Y, & Lee S.J. (2014). Radiation promotes malignant phenotypes through SRC in breast cancer cells. Cancer Science, 106(1), 78-85.

+ Open protocol

+ Expand

Chromatin Immunoprecipitation Antibody Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Normal rabbit IgG (12–370) and normal mouse IgG (12–371) were purchased from Millipore/Upstate. H3K79me3 (17–10,130), H3K36me3 (17–10,032), H3K27me1 (17–643), H3K4me3 (07–473), Acetyl-Histone H4 (17–630) and H3K9ac (17–658) antibodies were procured from Millipore. All of the above antibodies were used for ChIP analysis at 5 μg antibody per 30 μg chromatin. Oct-3/4 (sc-8628) and Sox2 (sc-17,319) antibodies were procured from Santa Cruz Biotechnology. Anti-Nanog (AF1997) was purchased from R&D Systems. Anti-H3 (ab1791) was procured from Abcam. All of the above antibodies were used for immunoblot analysis at 1:1000 dilution.

Jangid R.K., Kelkar A., Muley V.Y, & Galande S. (2018). Bidirectional promoters exhibit characteristic chromatin modification signature associated with transcription elongation in both sense and antisense directions. BMC Genomics, 19, 313.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!