Phos ephb1

Manufactured by Abcam

Phos-EphB1 is an antibody product that detects phosphorylated EphB1 protein. EphB1 is a member of the Eph receptor tyrosine kinase family and plays a role in cell-cell communication and signaling. The Phos-EphB1 antibody can be used to analyze the phosphorylation status of EphB1 in various experimental systems.

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Lab products found in correlation

Recombinant human ephrin b2 fc chimera protein, by R&D Systems (2 mentions) Ephb1, by Abcam (2 mentions) E cadherin, by Cell Signaling Technology (2 mentions) β actin, by Proteintech (2 mentions) Gapdh, by Proteintech (2 mentions) Cd133, by Abcam (1 mentions) Cisplatin p4394, by Merck Group (1 mentions) Cyclin a2, by Cell Signaling Technology (1 mentions) Recombinant human tgf β1, by Novoprotein (1 mentions) Smad2, by Proteintech (1 mentions) N cadherin, by Cell Signaling Technology (1 mentions)

Close spelling variants of this product

EphB1

2 protocols using phos ephb1

Antibody-Based Protein Analysis in Cancer

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Antibodies to proteins were obtained from the following sources: EphB1 (#ab129103) and phos-EphB1 (#ab61791) for Western blot were purchased from Abcam; EphB1 (#AF542) for immunohistochemistry was purchased from Novus Biologicals; Ki67, p53, CyclinA2 and E-cadherin (#3195) were from Cell Signaling Technology; β-actin (#60008-1-Ig) and GAPDH (#60004-1-Ig) were from Proteintech. Fluorescence-labeled ki67 and CD133 were purchased from Abcam, USA. Recombinant human Ephrin-B2 Fc chimera protein was purchased from R&D (7397-EB, RD Inc, MN, USA). Cisplatin (P4394) was purchased from SigmaAldrich (Sigma, MO, USA). Cells were treated with cisplatin at 10 μg/ml for A549 for 48 h.

Wang L., Peng Q., Xie Y., Yin N., Xu J., Chen A., Yi J., Shi W., Tang J, & Xiang J. (2022). Cell-cell contact-driven EphB1 cis- and trans- signalings regulate cancer stem cells enrichment after chemotherapy. Cell Death & Disease, 13(11), 980.

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Antibodies and Recombinant Proteins for Western Blot and IHC

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Antibodies to proteins were obtained from the following sources: EphB1 (#ab129103) and phos-EphB1 (#ab61791) for Western blot were purchased from Abcam; EphB1 (#AF542) for immunohistochemistry was purchased from Novus Biologicals; N-cadherin (#13116) and E-cadherin (#3195) were from Cell Signaling Technology; β-actin (#60008-1-Ig), Smad2 (#12570-1-AP) and GAPDH (#60004-1-Ig) were from Proteintech. Recombinant human Ephrin-B2 Fc chimera protein was purchased from R&D (7397-EB, RD Inc, MN, USA) and Recombinant Human TGF-β1 (#CA59) was purchased from Novoprotein.

Wang L., Peng Q., Sai B., Zheng L., Xu J., Yin N., Feng X, & Xiang J. (2020). Ligand-independent EphB1 signaling mediates TGF-β-activated CDH2 and promotes lung cancer cell invasion and migration. Journal of Cancer, 11(14), 4123-4131.

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