The cultures of MRSA with MUP, FA and/or RET resistance were incubated with lysostaphin (1 mg/mL) (Sangon Biotech, Shanghai, China) at 37 °C for 0.5 h. Then, the DNA was extracted according to the instructions of the bacterial genomic DNA kit (Tiangen Biotech, Beijing, China), and utilized as a template for PCR assays.
Bacterial genomic dna kit
Manufactured by Tiangen Biotech
Sourced in China
The Bacterial Genomic DNA Kit is a laboratory tool designed to efficiently extract and purify high-quality genomic DNA from bacterial samples. The kit utilizes a straightforward and reliable procedure to isolate DNA, making it suitable for a variety of downstream applications.
Automatically generated - may contain errors
Lab products found in correlation
3 protocols using bacterial genomic dna kit
1
Extracting MRSA Genomic DNA
2
Identification of Bacterial Strain JR48
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Strain JR48 was grown on solid nutrient agar (NA) or liquid nutrient broth (NB) medium at 28°C, and stored at -80°C in 30% (v/v) glycerol. To identify JR48, total genomic DNA was extracted using the Tiangen™ Bacterial Genomic DNA kit according to manufacturer’s instructions. Gyrase subunit B (gyrB) and 16S rRNA genes were amplified using universal primers (Table S1 Table S2
3
Bacterial Genome DNA Extraction and Sequencing
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Total DNA was extracted using a bacterial genomic DNA kit (Tiangen, China) according to the manufacturer's instruction. Sequencing was performed at Yuanquanyike Co. Ltd., China, using an Illumina Hiseq2000, using the 35 bp paired-end approach according to standard Illumina protocols. Of the more than 230 million 35-bp-long reads generated for BBMN68m, 97.67% were successfully mapped to the BBMN68 (GenBank Accession No. NC_014656.1) complete genome sequence using Maq software (default parameters). This resulted in 100× genome coverage. Single nucleotide polymorphisms (SNPs) were then identified and filtered using the pileup command for the SNP filter [19] .
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!