Hplc ms system

All chemicals were purchased from Sigma-Aldrich or Alfa Aesar unless otherwise stated and were used without further purification. The ¹H and ¹³C NMR spectra were recorded using a Bruker Advance III 500 MHz (11.4 T) spectrometer equipped with 5 mm PABBO probes and BVT-3000 temperature control unit. Chemical shifts δ are reported relative to TMS and were referenced using the residual proton solvent resonances. HPLC analyses and mass spectra were performed on a Waters HPLC-MS system equipped with a Waters 1525 binary pump. Analytical measurements were carried out on a Waters XTerra MS C18 (5 μm 4.6 × 100 mm) and on a Waters C18 XTerra Prep (5 μm 19 × 50 mm) for preparative purposes. Electrospray ionization mass spectra (ESI MS) were recorded using an SQD 3100 Mass Detector (Waters), operating in positive or negative ion mode, with 1% v/v formic acid in methanol as the carrier solvent.

All chemicals were purchased from Sigma-Aldrich or Alfa Aesar unless otherwise stated and were used without further purification. The ¹H and ¹³C NMR spectra were recorded using a Bruker Advance III 500 MHz (11.4 T) spectrometer equipped with 5mm PABBO probes and BVT-3000 temperature control unit. Chemical shifts are reported relative to TMS and were referenced using the residual proton solvent resonances. HPLC analyses and mass spectra were performed on a Waters HPLC-MS system equipped with a Waters 1525 binary pumps. Analytical measurements were carried out on a Waters Atlantis RPC18 column (5 μm 4.6 × 100 mm) and on a Waters Atlantis prep T3 OBD (5 μm 19 × 100 mm) for preparative purposes. Electrospray ionization mass spectra (ESI MS) were recorded using a SQD 3100 Mass Detector (Waters), operating in positive or negative ion mode, with 1% v/v formic acid in methanol as the carrier solvent.