Cto 20ac prominence column oven

α-Tocopherol consumption during the oxidation trial was analyzed
by the method from Aitta et al.^{29 (link)} NP-UHPLC-FLD
with a Shimadzu Nexera XR LC-30 HPLC instrument equipped with an LC-20AD
XR pump, SIL-20AC autosampler, CTO-20AC prominence column oven, and
RF-20A prominence fluorescence detector (Shimadzu, Kyoto, Japan) was
used. Excitation and emission wavelengths were 292 and 325 nm, respectively.
A Restek Pinnacle DB Silica UHPLC column (1.9 μm, 100 ×
2.1 mm, Bellefonte, PA) was applied for an 8 min separation run at
30 °C. Tray cooler temperature was set to 4 °C. The mobile
phase (isocratic, 0.4 mL/min) consisted of 2% 1,4-dioxane (Sigma-Aldrich,
Steinheim, Germany) and 98% heptane (Honeywell/Riedel de Haën,
Seelze, Germany). Samples were transferred to heptane for analysis.
For quantification, the α-tocopherol standard curve was prepared
from the α-tocopherol stock solution. Limit of detection (LOD)
and limit of quantification (LOQ) for the method were 0.08 and 0.26
ng per column, respectively. Chromatographic data were processed by
LabSolutions 5.93 (Shimadzu Corporation, Kyoto, Japan).

The bisphenol A concentration in the filtrate samples was measured by means of a high-performance liquid chromatography (HPLC) system coupled with a diode array detector (SPD-20A Prominence UV Detector, SIL-20AC Prominence autosampler, CTO-20AC Prominence Column oven, DGU-20A5 Prominence degasser, LC-20AT pump, Shimadzu, Tokyo, Japan). The samples were separated by reverse phase (RP) chromatography using a C18 Luna (Phenomenex, Torrance, CA, USA) column (5 µm, 250 × 4.6 mm). The HPLC method was obtained by Tursi et al. [85 (link)]. The mobile phase was a mixture of water/acetonitrile (35/65, v/v) at a flow rate of 1 mL/min using a UV wavelength at 227 nm. The solvents were eluted isocratically, and the injection volume was kept at 20 µL. A calibration curve was prepared for the quantification of BPA in water by using standards at different concentrations (from 0.5 ppm to 50 ppm). No traces of byproducts amenable to polymer degradation and/or the release of impurities from the MWCNTs-TiO₂ nanomaterial were detected by HPLC analysis.

The Spectrophotometer Ultraviolet-Visible V-530 was purchased from Jasco (Tokio, Japan). The HPLC Nexera2 is composed of the following units: an LC-30AD liquid chromatograph, an SIL-30AC autosampler, a CTO-20AC prominence column oven, an SPD-M30A diode array detector, and a DGU-20A5R degassing unit, purchased from Shimadzu (Kyoto, Japan). The gas chromatograph (GC) CP3800 was purchased from Varian (Palo Alto, CA, USA).