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Anti pin1

Manufactured by Cell Signaling Technology
Sourced in Sweden

Anti‐Pin1 is a lab equipment product that functions as an antibody specific to the Pin1 protein. Pin1 is a peptidyl‐prolyl cis/trans isomerase that plays a role in various cellular processes. The Anti‐Pin1 product can be used to detect and study the Pin1 protein in research applications.

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2 protocols using anti pin1

1

Silencing Pin1 in Hep3B Cells

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Hep3B cells were seeded in 6‐well plates at 2 × 105 cells/well. DP7‐C and GalNAc‐Pin1 siRNA complex was incubated with the Hep3B cells at doses of 1 μg GalNAc‐Pin1 siRNA. Hep3B cells were harvested 48 hours later for analysis. Total RNA was extracted with a kit (ForeGene). qPCR reactions were performed on a Bio‐Rad CFX96 system.
The western blot results better showed the silencing efficiency of the siRNA. The protein samples were probed with anti‐GAPDH (Cell Signaling Technology, CST), anti‐Pin1 (CST), anti‐Beta Tubulin (CST), anti‐Lamin B (CST), and anti‐Exportin 5 (CST) antibodies. The cells were incubated with primary antibodies at 4°C overnight. Then, the membranes were incubated with secondary antibodies (CST) for 60 minutes and developed using the ECL detection system (Vazyme).
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2

Western Blotting for Protein Expression

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Western blotting for the expression of E-cadherin, vimentin, CD44, LC3A, MET, ATG5, total EGFR, phosphorylated EGFR, poly(ADP-ribose) polymerase-1 (PARP-1), γH2AX, total AKT, phospho-AKT, total ERK1/2, phospho-ERK1/2, Mcl-1, Bim, and β-actin was performed as described previously. 6, 13 Additional primary antibodies for immunoblotting were anti-ZEB1 (HPA027524; 1:1000 dilution; Atlas Antibodies, Stockholm, Sweden), anti-ATG7 (D12B11; 1:1000 dilution; Cell Signaling), anti-Pin1 (1:1000 dilution; Cell Signaling), anti-Survivin (71G4B7; 1:1000 dilution; Cell Signaling), and anti-Puma (EP512Y; 1:2000 dilution; Abcam Japan, Tokyo, Japan). Band intensity levels on X-ray films were normalized to β-actin using the Image J software (National Institutes of Health, Bethesda, MD, USA).
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