The western blot results better showed the silencing efficiency of the siRNA. The protein samples were probed with anti‐GAPDH (Cell Signaling Technology, CST), anti‐Pin1 (CST), anti‐Beta Tubulin (CST), anti‐Lamin B (CST), and anti‐Exportin 5 (CST) antibodies. The cells were incubated with primary antibodies at 4°C overnight. Then, the membranes were incubated with secondary antibodies (CST) for 60 minutes and developed using the ECL detection system (Vazyme).
Anti pin1
Anti‐Pin1 is a lab equipment product that functions as an antibody specific to the Pin1 protein. Pin1 is a peptidyl‐prolyl cis/trans isomerase that plays a role in various cellular processes. The Anti‐Pin1 product can be used to detect and study the Pin1 protein in research applications.
Lab products found in correlation
2 protocols using anti pin1
Silencing Pin1 in Hep3B Cells
The western blot results better showed the silencing efficiency of the siRNA. The protein samples were probed with anti‐GAPDH (Cell Signaling Technology, CST), anti‐Pin1 (CST), anti‐Beta Tubulin (CST), anti‐Lamin B (CST), and anti‐Exportin 5 (CST) antibodies. The cells were incubated with primary antibodies at 4°C overnight. Then, the membranes were incubated with secondary antibodies (CST) for 60 minutes and developed using the ECL detection system (Vazyme).
Western Blotting for Protein Expression
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