Zeta potential and particle size distribution measurements were performed using a nano-ZS90 Zetasizer (Malvern instruments Ltd., Malvern, UK). Particle size distributions were measured after equilibration for 2 min at 25 °C using disposable polystyrene cuvettes containing dispersions (0.01 g/mL). The data were recorded and analyzed using the Dispersion Technology Software v. 5.0 (Malvern Instruments). For zeta potential measurement, samples (0.5 wt% particle dispersion) were deposited into clear disposable zeta cells and the results were automatically generated with the help of Dispersion Technology Software v. 5.0 (Malvern Instruments). The sample pH, which was modified using NaOH or HCl (0.1 M), was determined using a PB-10 pH meter (Sartorius AG, Goettingen, Germany) at 25 °C. No additional electrolytes were added to each sample for the measurement of zeta potential and particle size distribution.
Dispersion technology software v5
Manufactured by Malvern Panalytical
Sourced in United Kingdom
Dispersion Technology Software v5.0 is a software application developed by Malvern Panalytical for the analysis and characterization of particle size and particle size distribution. The software provides a comprehensive suite of tools for the interpretation and reporting of particle size data obtained from various Malvern Panalytical instruments.
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Lab products found in correlation
Malvern zetasizer nano z, by Malvern Panalytical (3 mentions)
Malvern zetasizer nano, by Malvern Panalytical (2 mentions)
Zetasizer nano, by Malvern Panalytical (1 mentions)
Zetasizer nano zs series, by Malvern Panalytical (1 mentions)
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Zetasizer nano zs90, by Malvern Panalytical (1 mentions)
Pb 10 ph meter, by Sartorius (1 mentions)
9 protocols using dispersion technology software v5
1
Particle Characterization: Zeta Potential and Size
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Measuring ζ-potential of AcTTR1-GGE Fibrils
3
Measuring ζ-potential of AcTTR1-GGE Fibrils
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The ζ-potential of AcTTR1-GGE fibrils was determined with a Malvern ZetaSizer Nano (Malvern Instruments) by taking the average of four samples of fibrils at a final concentration of 20 μg/ml in ultrapure water at pH 7.0 using a disposable zeta cell. Data were then analyzed using the Malvern Instrument Dispersion Technology Software v. 5.03.
4
Characterizing Extracellular Vesicle Size Distribution
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The size distribution of vesicles present in EV-preparations was measured by DLS. Briefly, before to DLS measurements, the EVs-preparations (5 μL, n = 12) were extended with 0.1 μm filtered-PBS (45 μL) and shaked to disaggregate possible clumps of EVs. Then, 50 μL of each sample were added to a cuvette with a 10 mm pathlength. The analysis was conducted using a Nano Zetasizer (Malvern Instruments, Malvern, UK) at RT, operating at 633 nm and recording the back scattered light at an angle of 173°. The light scattering was recorded for 150 s, with 3 replicate measurements for each sample. Dispersion Technology Software v.5.10 (Malvern Instruments) was used to transform DLS signal intensity to size distribution. The peak maximum of the gaussian function was used to estimate EV diameter (nm). The intensity-based distribution was re-calculated to a volume. The results were expressed as volume and intensity size distribution.
5
ζ-Potential Measurement of Aqueous Suspensions
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The ζ-potential of IOF and SOF samples after treatments (see Preparation of Aqueous Suspension and Emulsion paragraph) was determined using an electrophoretic light scattering instrument (Zetasizer Nano ZS series, Malvern Instruments Ltd, Malvern, UK). Before measurements, samples were diluted to 0.5% using Milli-Q water or buffer to avoid multiple scattering effects. After loading the samples in the folded capillary cells and inserting into the instrument, they were equilibrated at 25 °C for 2 min. Next, three repeated measurements obtained from 30 continuous readings on each sample were recorded. ζ-Potential was calculated applying the Smoluchowski model on the acquired electrophoretic data using Dispersion Technology Software v. 5.10 (Malvern Instruments). Each measurement was repeated three times.
6
Hydrodynamic Size Analysis of NPCB
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The hydrodynamic size distribution of NPCB suspensions was determined by DLS using Malvern Zetasizer Nano ZS (Malvern Instruments, UK). Data were analyzed on Dispersion Technology Software v5.0 (Malvern Instruments). The size distribution was determined directly on the instillation dispersions at 25°C in 1 ml disposable polystyrene cuvettes. All data were obtained based on six consecutively repeated analyses of the same sample with no pause. For calculation of hydrodynamic size, we used the refractive (Ri) and absorption indices (Rs) of 2.020 and 2.000, respectively, for Printex 90 and standard optical and viscosity properties for H2O.
7
Dynamic Light Scattering for Nanoparticle Characterization
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The hydrodynamic size distributions of the different dispersions were determined by dynamic light scattering (DLS) using a Malvern Zetasizer Nano ZS (Malvern Instruments, UK) and the Dispersion Technology Software v5.0 (Malvern Instruments, UK). Size distributions were measured at 25°C directly on the instillation suspensions. Data were recorded as the mean value of six consecutively repeated analyses of each sample. For the calculation of hydrodynamic size and dispersity, standard optical and viscosity properties for water were used. Refractive and absorption indices of 2.020 and 2.000 were used for CB. These values were 2.903 and 0.10, respectively, for TiO2.
8
Dynamic Light Scattering of Nanoparticles
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The hydrodynamic size distributions in suspensions were determined by dynamic light scattering (DLS) using Malvern Zetasizer Nano ZS (Malvern Instruments Ltd, UK). Data were analysed on Dispersion Technology Software v5.0 (Malvern Instruments Ltd). The size distribution was determined directly after sonication on the instillation dispersions at 25°C in 1ml disposable polystyrene cuvettes. All data were obtained based on six consecutively repeated analyses of the same sample with no pause. For calculation of hydrodynamic size, we used the refractive (Ri) and absorption indices (Rs) of 2.903 and 0.10, respectively, for both rutile TiO2 NPs and standard optical and viscosity properties for H2O.
9
Dynamic Light Scattering of Suspensions
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The hydrodynamic size distributions of the instillation suspensions were determined by Dynamic Light Scattering (DLS) using a Malvern Zetasizer Nano ZS (Malvern Instruments, UK) at 25 C. Analysis was done using the Dispersion Technology Software v5.0 (Malvern Instruments, UK). Hydrodynamic size was calculated using a dispersion refractive index of 1.33, a materials refractive index of 2.1, a viscosity 0.89 cP and a material absorption value of 2.0.
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