A22220

Manufactured by Abbkine

Sourced in United States, China

The A22220 is a laboratory instrument designed for the detection and quantification of various analytes. It utilizes a specific technology to perform these measurements. The core function of the A22220 is to provide accurate and reliable data to support research and analysis activities in a laboratory setting.

Automatically generated - may contain errors

Lab products found in correlation

A23220, by Abbkine (2 mentions) Inverted microscope, by Olympus (1 mentions) A22210, by Abbkine (1 mentions) Ab72538, by Abcam (1 mentions) Mab377, by Merck Group (1 mentions) D9542, by Merck Group (1 mentions) Ab26350, by Abcam (1 mentions) Ab36823, by Abcam (1 mentions) Sc 7867, by Santa Cruz Biotechnology (1 mentions) Goat anti mouse igg fitc sc 2010, by Santa Cruz Biotechnology (1 mentions) Goat anti rabbit igg cy3, by Abbkine (1 mentions) Tunel staining kit, by Roche (1 mentions) A2547, by ABclonal (1 mentions) A23240, by Abbkine (1 mentions) Ab27156, by Abcam (1 mentions)

4 protocols using a22220

Immunocytochemistry of Neuronal Markers

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells in 96-well plates were fixed with 4% paraformaldehyde (PFA) for 20 min at room temperature (RT). Fixed cells were then rinsed, permeabilized with 0.2% Triton X-100 for 10 min, blocked with 3% bovine serum albumin (BSA) for 30 min, stained with appropriate primary antibodies, and incubated overnight at 4 °C. After washing three times with PBS for 5 min each time, the respective secondary antibodies were added and the cells were incubated at RT for 1 h in the dark. Next, the cells were washed three times with PBS for 5 min each and stained with 0.5 mg/ml DAPI at RT for 10 min. After another triple washing, the images were photographed under an inverted microscope (Olympus, Japan). The fluorescence-positive neurites were traced and graphed using the filament tracing function of Imaris software (v.9.0.1, Bitplane).
The following antibodies were used: mouse anti-β-III Tubulin (1:100, Beyotime Cat# AT809, RRID: AB_2893434); rabbit anti-MAP2 (1:50, Signalway Cat# 21636, RRID: AB_2923039); rabbit anti-NeuN (1:400, Abcam Cat# ab177487, RRID: AB_2532109); Cy3, anti-mouse IgG (1:400, Abbkine Cat# A22210, RRID: AB_2923040); Cy3, anti-rabbit IgG (1:400, Abbkine Cat# A22220, RRID: AB_2923041); and DyLight 488, anti-rabbit IgG (1:200, Abbkine Cat# A23220, RRID: AB_2737289).

Zhang Z.Y., Zuo Z.Y., Liang Y., Zhang S.M., Zhang C.X., Chi J., Fan B, & Li G.Y. (2023). Promotion of axon regeneration and protection on injured retinal ganglion cells by rCXCL2. Inflammation and Regeneration, 43, 31.

+ Open protocol

+ Expand

Immunofluorescent Detection of HIPK3

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were fixed with 4% paraformaldehyde for 20 min and then permeabilized by incubating with 150 μl 0.5% Triton X-100 for 15 min at room temperature. After blocking for 60 min in normal goat serum (ZGGB-BIO, China), cells were incubated overnight with an anti-HIPK3 antibody (ab72538, Abcam, U.K.; 1:150 dilution) diluted in 5% bovine serum albumin at 4°C. The next day, cells were incubated for 60 min with a goat anti‐rabbit secondary antibody (A22220, Abbkine, U.S.A.; 1:200 dilution), counterstained with DAPI, and imaged on a fluorescence microscope.

Wang X., Zhang T., Zhai J., Wang Z., Wang Y., He L., Ma S., Xing H, & Guo Y. (2023). MiR-21 attenuates FAS-mediated cardiomyocyte apoptosis by regulating HIPK3 expression. Bioscience Reports, 43(9), BSR20230014.

+ Open protocol

+ Expand

Immunostaining of DNA Damage Response Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

For immunostaining, sections from 6-month-old mice were stained for RNF8, MDC1, 53BP1, BRCA1 and NeuN. Sections were boiled in 0.01 M sodium citrate (pH 6.0) for 10 min to retrieve the antigens. Standard immunostaining procedures were used. The following antibodies were used: anti-γH2AX [phosphor S139] (ab26350, Abcam, 1:400 dilution), rabbit anti-MDC1 (ABC155, Millipore, 1:500 dilution), anti-53BP1 (ab36823, Abcam), anti BRCA1 (H-100) (sc-7867, Santa Cruz, 1:200 dilution), and anti-NeuN (clone A60, MAB377, Millipore, 1:500 dilution). For secondary detection, goat anti-mouse IgG-FITC (sc-2010, Santa Cruz, 1:400 dilution) and goat anti-rabbit IgG-Cy3 (A22220, abbkine, 1:400 dilution) were used. Then, the neuronal nuclei were counterstained with 4',6-diamidino-2-phenylindole (DAPI; D9542, Sigma). After a final wash with PBS, sections were mounted in anti-fading medium (glycerin containing p-phenylenediamine). All images were obtained with an Olympus fluorescence microscope (BX53).

Ouyang S., Song Y., Tian Y., Chen Y., Yu X, & Wang D. (2015). RNF8 deficiency results in neurodegeneration in mice. Neurobiology of aging, 36(10), 2850-2860.

+ Open protocol

+ Expand

Immunofluorescence Analysis of DNA Damage and Inflammation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded into a 24‐well plate containing coverslips before receiving IR. Twelve hours after 5 Gy radiotherapy, the cells were harvested, fixed with 1% paraformaldehyde, permeabilized with 0.5% Triton, blocked with 5% BSA and then incubated with primary antibodies at 4°C overnight. The primary antibodies were anti‐double stranded DNA (dsDNA; 1:200, ab27156, Abcam), anti‐cGAS (1:200, #79978, Cell Singling Technology) and anti‐NF‐κB P65 (1:200, A2547, ABclonal, Wuhan, Hubei, China). The next day, the cells were incubated with secondary antibodies for 1 h at room temperature followed by 2‐(4‐Amidinophenyl)‐6‐indolecarbamidine dihydrochloride (DAPI; Abbkine, Beijing, China) staining for 5 min. The secondary antibodies were Cy3, goat anti‐rabbit IgG (1:500, A22220, Abbkine, Beijing, China), DyeLight 488 goat anti‐mouse IgG (1:500, A23240, Abbkine), and DyeLight 488 goat anti‐rabbit IgG (1:500, A23220, Abbkine). Cover slides were then mounted for microscopy. For terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay, sections were stained using the TUNEL staining kit (11684817910, Roche, Germany) according to the manufacturer's protocols. For the micronuclei assay, cells were stained with DAPI with antifade reagent for microscopy and scoring. At least 150 nuclei were counted in 10 fields.

Liu C., Wang X., Qin W., Tu J., Li C., Zhao W., Ma L., Liu B., Qiu H, & Yuan X. (2023). Combining radiation and the ATR inhibitor berzosertib activates STING signaling and enhances immunotherapy via inhibiting SHP1 function in colorectal cancer. Cancer Communications, 43(4), 435-454.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!