Rabbit anti vangl1

The dilution and sources for primary antibodies used were as follows: rabbit anti-Vangl1 (1:200, Sigma), rabbit anti-Vangl2 (1:200, Santa Cruz), goat anti-Frizzled6 (1:200, R&D systems), goat anti-Frizzled3 (1:100, R&D systems), rat anti-Frizzled3 (1:100, R&D systems), rat anti-E-Cadherin (1:200, Invitrogen) and mouse anti-E-cadherin (1:200, BD Transduction Laboratories), mouse anti-acetylated-α-tubulin (1:500, Sigma), rabbit anti-phospho-Histone H3 (Ser10) (1:200, Millipore) and mouse anti-β-catenin (1:100, BD Transduction Laboratories^™). The following secondary antibodies were used at 1:500 and purchased from Molecular Probes (now ThermoFisher): Alexa 488, 594 donkey-anti-rabbit, Alexa 488, 546 donkey-anti-goat, Alexa 594, 633 donkey-anti-rat, Alexa 647 chicken-anti-rat and Streptavidin Alexa 594 conjugate. Phalloidin conjugated to Alexa 633 was used at 1:50. Rhodamine-conjugated Dolichos Biflorus Agglutinin lectin (DBA, 1:50, Vector Laboratories) and goat anti-AQP2 (1:200, Santa Cruz) were used to stain collecting duct. Fluorescein-conjugated Lotus Tetragonolobus lectin (LTL, 1:200, Vector Laboratories) and biotinylated Lotus Tetragonolobus lectin (1:200, Vector Laboratories) were used to stain proximal tubules. DAPI (300nM, Sigma), Sytox Green (1:20,000, Invitrogen) and 7-aminoactinomycin D (7-AAD, 1:40, Invitrogen) were used to stain nuclei.