Image analysis was performed using ImageJ 1.46m and Adobe Photoshop CS5. Adjustment of image brightness or contrast was performed in some cases but without misrepresenting data. Lesion area was measured using the freehand selection tool inside FluoroMyelin negative areas. Lesion size and volume calculations were based on the number of sections collected for each lesion multiplied by their thickness and their average lesion area. Fluorescence intensity for specific antibodies within the lesion area was measured using the mean grey value. All measurements were normalized by the background mean gray value obtained from a section of normal appearing white matter adjacent to the lesion. When calculating remyelination by EGFP+ oligodendrocytes at 28 dpi, the particle analysis tool was used to measure the total cell area (including cell body and processes) within the lesion shadow (EGFP−, MBP+). This value was then divided by the total shadow area, to obtain the percentage covered by EGFP+ oligodendrocytes. Statistical tests were performed using Graph Pad Prism software.
Imagej 1.46m
Manufactured by Adobe
ImageJ 1.46m is an open-source image processing program designed for scientific multidimensional images. It provides a platform for the visualization, analysis, and processing of images.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using imagej 1.46m
1
Quantitative Analysis of Remyelination
2
Quantitative Analysis of Remyelination
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Image analysis was performed using ImageJ 1.46m and Adobe Photoshop CS5. Adjustment of image brightness or contrast was performed in some cases but without misrepresenting data. Lesion area was measured using the freehand selection tool inside FluoroMyelin negative areas. Lesion size and volume calculations were based on the number of sections collected for each lesion multiplied by their thickness and their average lesion area. Fluorescence intensity for specific antibodies within the lesion area was measured using the mean gray value. All measurements were normalized by the background mean gray value obtained from a section of normal appearing white matter adjacent to the lesion. When calculating remyelination by EGFP+ oligodendrocytes at 28 dpi, the particle analysis tool was used to measure the total cell area (including cell body and processes) within the lesion shadow (EGFP−, MBP+). This value was then divided by the total shadow area, to obtain the percentage covered by EGFP+ oligodendrocytes. Statistical tests were performed using Graph Pad Prism software.
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