Ethanol

Manufactured by Carlo Erba

Sourced in Italy, France, Germany

Ethanol is a colorless, volatile liquid that is commonly used as a solvent in laboratory settings. It is a chemical compound with the formula C2H5OH, consisting of a hydroxyl group (-OH) attached to an ethyl group (-CH2CH3). Ethanol is a flammable substance, and its primary function is to serve as a versatile solvent for various organic compounds, making it a valuable tool in a wide range of laboratory applications.

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Lab products found in correlation

Acetonitrile, by Carlo Erba (10 mentions) Methanol, by Carlo Erba (10 mentions) Acetone, by Carlo Erba (10 mentions) Gallic acid, by Merck Group (9 mentions) Chloroform, by Carlo Erba (6 mentions) N hexane, by Carlo Erba (6 mentions) Tetrahydrofuran, by Carlo Erba (5 mentions) Hydrochloric acid (hcl), by Carlo Erba (5 mentions) Potassium persulfate, by Merck Group (5 mentions) Acetic acid, by Carlo Erba (4 mentions) Milli q system, by Merck Group (4 mentions) Sodium carbonate, by Carlo Erba (4 mentions) Ethyl acetate, by Carlo Erba (4 mentions) Methanol, by Avantor (4 mentions) Sodium hydroxide (naoh), by Carlo Erba (4 mentions) Methanol, by Merck Group (4 mentions) Quercetin, by Merck Group (4 mentions) Dimethyl sulfoxide (dmso), by Carlo Erba (4 mentions) Chloroform, by Merck Group (4 mentions) Sodium hydroxide (naoh), by Merck Group (4 mentions)

Close spelling variants of this product

Absolute ethanol (28 citations) Ethanol absolute anhydrous (27 citations)

57 protocols using ethanol

HPLC Separation of Betulinic and Oleanolic Acids

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HPLC grade m ethanol and isopropanol were purchased from Sigma-Aldrich; acetonitrile, ethanol, and water were purchased from Carlo Erba Reagents; and ethyl acetate and acetone were purchased from VWR. Betulinic acid (purity ≥ 98 %) and oleanolic acid (purity ≥ 98 %) were purchased from AK Scientific. All solvents and solutes were used as purchased without further
purification. An Acclaim C30 column (250 × 4.6 mm, μm, pore size: 200 Å, surface area: 𝑑 p = 5 200 m 2 /g, carbon load: 13 %, monomeric bonded phase) was purchased from Thermo Fisher Scientific, Inc.

Azenha I.S., Aniceto J.P., Ribeiro D.P., Mendes A., & Silva C.M. (2021). Reversed-phase chromatographic separation and downstream precipitation of lupane- and oleanane-type triterpenoids: Experiments and modeling based on the method of moments. Separation and Purification Technology, 260, 118208-118208.

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Synthesis of Nickel Nanocrystals

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Ni⁰ NCs
were synthesized following the procedure reported by Carenco et al.^{16 (link)} In a 25 mL flask, 1 equiv of nickel acetylacetonate
[Ni(acac)₂, 95%, purchased by Sigma-Aldrich] and 10 equiv
of OLAM were mixed and degassed in a standard Schlenk line under vacuum
for 2 h at 100 °C. Afterward, the atmosphere was switched to
argon and the solution was heated up to 220 °C. The reaction
time was 2 h. Then, the solution was cooled down to room temperature.
Ni⁰ NCs were washed three times with a mixture of hexane
(99%, purchased from Sigma-Aldrich) and ethanol (99.9%, purchased
from Carlo Erba Reagents), each rinsing followed by centrifugation
(4500 rpm, 10 min) and discarding of the supernatant. The Ni⁰ NCs were dispersed in hexane for further characterizations/experiments.

Bellato F., Ferri M., Annamalai A., Prato M., Leoncino L., Brescia R., De Trizio L, & Manna L. (2022). Colloidal Synthesis of Nickel Arsenide Nanocrystals for Electrochemical Water Splitting. ACS Applied Energy Materials, 6(1), 151-159.

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PLGA-based Nanoparticle Synthesis Protocol

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Poly (D,L-lactide-co-glycolide) (PLGA) (50:50 Resomer RG 502H inherent viscosity 0.16–0.24 dL/g) was purchased from Boehringer Ingelheim (Ingelheim, Germany). Polyethyleneimine (PEI, MW = 10–25 kDa branched), poloxamer 188 (Pluronic® F68), Bradford reagent, potassium phosphate dibasic and potassium phosphate monobasic, sodium azide, sodium chloride, copper (II) sulphate and albumin from chicken egg white (OVA), were purchased from Sigma-Aldrich. Sodium hydroxide was provided from Delchimica Scientific Glassware. Ethanol (96 %), phosphoric acid (85 %), acetic acid, acetonitrile and tetrahydrofuran were purchased from Carlo Erba Reagenti (Milan, Italy).

Petrizzo A., Conte C., Tagliamonte M., Napolitano M., Bifulco K., Carriero V., De Stradis A., Tornesello M.L., Buonaguro F.M., Quaglia F, & Buonaguro L. (2015). Functional characterization of biodegradable nanoparticles as antigen delivery system. Journal of Experimental & Clinical Cancer Research : CR, 34, 114.

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Solvents and Reagents Sourcing Protocol

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Dimethyl sulfoxide (DMSO), m ethanol, and ethanol were obtained from Carlo Erba Reagent (Milan, Italy) in their highest commercially available purity grade. DMT (code #31298-250MG), TPA (code #40818-100MG), and all other reagents, if not differently specified, were purchased from Merk Life Science (Milan, Italy).

Molonia M.S., Muscarà C., Speciale A., Salamone F.L., Toscano G., Saija A, & Cimino F. (2022). The p-Phthalates Terephthalic Acid and Dimethyl Terephthalate Used in the Manufacture of PET Induce In Vitro Adipocytes Dysfunction by Altering Adipogenesis and Thermogenesis Mechanisms. Molecules, 27(21), 7645.

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Analytical-Grade Solvents for Phenolic Compounds

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All the solvents and chemicals used in this study were of analytical grade. M ethanol, ethanol, acetonitrile LC-MS grade, and 85% phosphoric acid were purchased from Merck (Darmstadt, Germany, ethanol from Carlo Erba Reagenti (Milan, Italy). Standards of phenolic compounds were obtained from Extrasynthese (Genay, France), TransMIT (Giessen, Germany), and Merck-Sigma-Aldrich (Milan, Italy). Ultrapure water (18 MΩ·cm) was obtained with a Milli-Q Advantage A10 System apparatus (Millipore, Milan, Italy).

Spissu Y., Gil K.A., Dore A., Sanna G., Palmieri G., Sanna A., Cossu M., Belhadj F., Gharbi B., Pinna M.B., Barberis A., Tuberoso C.I, & D’hallewin G. (2022). Anti- and Pro-Oxidant Activity of Polyphenols Extracts of Syrah and Chardonnay Grapevine Pomaces on Melanoma Cancer Cells. Antioxidants, 12(1), 80.

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Fabrication of Silk-Coated Alginate Microcarriers

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Alginate microcarriers were produced according to the following procedure: briefly, a 1% w/v sodium alginate (Sigma-Aldrich, Milan, Italy) aqueous solution was dropped into a bath of 100 mM calcium chloride (Sigma-Aldrich) water solution using an automatic encapsulator (Encapsulator VAR V1, Nisco Engineering AG, Zurich, Switzerland, 0.17-mm diameter nozzle). Bombyx mori cocoons were degummed in autoclave, dried at room temperature, and solubilized in phosphoric acid/formic acid (80:20 v/v) (Sigma-Aldrich). Fibroin solution was dialyzed against water (polyethersulfone membrane, cut off 12 kDa, Visking, London, UK), obtaining a 1.5% w/v silk fibroin aqueous solution. Alginate microcarriers coated with silk fibroin were obtained adding alginate microcarriers into silk fibroin solution for 5 min. Microcarriers were collected by filtration and immersed in 96% (v/v) ethanol (Carlo Erba Reagents, Milan, Italy) for 15 min to induce silk conformational transition. Silk/alginate microcarriers presented β-sheet fibroin conformation (verified by Fourier Transform Infrared Spectroscopy), spherical geometry, and average diameter of about 400 μm in laser light scattering analyzer (Mastersizer 2000, Malvern Instruments Ldt, Worcestershire, UK).

Duchi S., Piccinini F., Pierini M., Bevilacqua A., Torre M.L., Lucarelli E, & Santi S. (2017). A new holistic 3D non-invasive analysis of cellular distribution and motility on fibroin-alginate microcarriers using light sheet fluorescent microscopy. PLoS ONE, 12(8), e0183336.

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Colloidal Synthesis of Tellurium Nanoparticles

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Materials. Copper (II) acetylacetonate (Cu(acac)2, 97%), tri-n-octylphosphine (TOP, 97%), 1,2 dichlorobenzene (DCB, 99%) and octylamine (Oct, 99,5%) were purchased from Sigma-Aldrich. Tellurium powder (99.999%) was purchased from Strem Chemicals. Ethanol (anhydrous, ≥99.8%), and chloroform (anhydrous, ≥99%) were purchased from Carlo Erba reagents. All chemicals were used as received without further purification and all reactions were carried out under nitrogen using standard air-free techniques.

Mugnaioli E., Gemmi M., Tu R., David J., Bertoni G., Gaspari R., De Trizio L, & Manna L. (2018). Ab Initio Structure Determination of Cu_{2- x}Te Plasmonic Nanocrystals by Precession-Assisted Electron Diffraction Tomography and HAADF-STEM Imaging. Inorganic chemistry, 57(16).

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Phenolic Compounds: Analytical Standards

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Cyanidin, peonidin 3-o-glucoside, malvidin-3-o-glucoside, quercetin, quercetin 3-o-glucoside, coumaric acid, gallic acid, syringic acid, epi catechin, catechin, and kaempferol (all analytical standard) were from Merck (Milan, Italy). M ethanol, ethanol, and acetonitrile (all LC/MS grade solvents), ortophospforic acid 85%, Folin–Ciocalteu reagent and sodium carbonate were bought from Carlo Erba (Milan, Italy). Water was purified through a Milli-Q system from Millipore (conductivity: 18.2 MΩ cm at 25 °C; Milford, MA, USA).

Castangia I., Aroffu M., Fulgheri F., Abi Rached R., Corrias F., Sarais G., Bacchetta G., Argiolas F., Pinna M.B., Murru M., Manca M.L., Manconi M, & Nácher A. (2024). From Field to Waste Valorization: A Preliminary Study Exploring the Impact of the Wine Supply Chain on the Phenolic Profile of Three Sardinian Pomace Extracts. Foods, 13(9), 1414.

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Antioxidant and Protein Oxidation Assays

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DPPH (1,1-diphenyl-2-picryl-hydrazyl radical), Trolox (6-hydroxy-2,5,7,8-tetramethylchromane-2-carboxylic acid), BHT (butylhydroxytoluene), TBA (2-thiobarbituric acid), TMP (1,1,3,3-tetra-methoxy-propane), DNPH (di-nitrophenol hydrazine), and BSA (bovine serum albumin) were purchased from Sigma-Aldrich (Steinheim, Germany).
Guanidine hydrochloride, TCA (trichloroacetic acid), acetonitrile, ethanol, ethyl acetate were purchased from Carlo Erba Reagents (Val de Reuil Cedex, France).

Rinaldi S., Palocci G., Di Giovanni S., Iacurto M, & Tripaldi C. (2021). Chemical Characteristics and Oxidative Stability of Buffalo Mozzarella Cheese Produced with Fresh and Frozen Curd. Molecules, 26(5), 1405.

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Streptozotocin-Induced Diabetes Protocol

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Streptozotocin (STZ) (Spectrochem Pvt, Ltd, Mumbai, India), distilled water (Tigray regional laboratory, Mekelle, Ethiopia), ethanol (Carlo Erba Reagents, Italy), Tween 80 (Atlas Chemical Industries Inc., USA), glucose standard strip/kits and glucometer (ACCU-CHEK Active, Germany) and Glibenclamide (Alfa Aesar, Great Britain) were some of the chemicals used during the experiment. All other chemicals used were of analytical grade.

Gebremeskel L., Beshir Tuem K, & Teklu T. (2020). Evaluation of Antidiabetic Effect of Ethanolic Leaves Extract of Becium grandiflorum Lam. (Lamiaceae) in Streptozotocin-Induced Diabetic Mice. Diabetes, Metabolic Syndrome and Obesity: Targets and Therapy, 13, 1481-1489.

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