7890a gc system

Manufactured by Agilent Technologies

Sourced in United States, Germany, Spain

The 7890A GC system is a gas chromatograph designed for analytical applications. It is capable of performing separations and quantitative analysis of complex mixtures. The system includes an oven, injector, and detector to facilitate the chromatographic process.

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GC 7890A (171 citations) Agilent 7890A GC system (82 citations) 7890A Network GC system (4 citations) GC systems (3 citations) 7890A/5975C-GC/MSD system (3 citations) 7890A GC-System apparatus (2 citations) Agilent 7890A/5977B GC/MSD system with 19091S-433 capillary column (2 citations) Agilent 7890A-5975 GC-MS system (2 citations) Model 7890A network GC system (2 citations) Agilent 7890A-5975C GC-MS network system (2 citations)

268 protocols using 7890a gc system

Comprehensive GC×GC-MS Analysis of Environmental Samples

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We analyzed our environmental samples with a Leco Corp GC×GC-μECD
instrument equipped with a modified Agilent 7890A GC system having
a split/splitless injector and a dual-stage, quadruple-jet modulator.
The Leco GC×GC had a 30 m length, 0.25 mm inner diameter (i.d.),
0.25 μm film thickness RTX-1 column (Restek, USA) as the first
dimension and a 2 m length, 0.1 mm i.d., 0.1 μm film thickness
BPX-50 column as the second dimension (Restek).
GC×GC-ENCI-TOFMS
and GC×GC-EI-TOFMS analyses were both performed using a Zoex
(Zoex Corp.) instrument. This instrument was a modified Agilent 7890A
GC system with a loop thermal modulator supplied by Zoex. The TOFMS
was made by TOFWERK, Switzerland, and it was equipped with both an
EI source and ENCI source. The column set and temperature program
were similar to those used for GC×GC-μECD measurements.
When used with either an EI or ENCI source, the TOFMS exhibited a
mass precision of ± 5 mmu for the target masses that we investigated.
Further instrument details are reported in Section S6 of the Supporting Information. These analyses provided us
with the bases for the comparison of the combination of GC×GC-μECD
and GC×GC-ENCI-TOFMS with conventional GC×GC-EI-TOFMS.

Samanipour S., Dimitriou-Christidis P., Nabi D, & Arey J.S. (2017). Elevated Concentrations of 4-Bromobiphenyl and 1,3,5-Tribromobenzene Found in Deep Water of Lake Geneva Based on GC×GC-ENCI-TOFMS and GC×GC-μECD. ACS Omega, 2(2), 641-652.

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Sludge Characterization and Biogas Composition Analysis

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COD and solid concentrations were measured in accordance with Standard Methods (APHA, 1998) . Sludge dewaterability was measured with capillary suction time (CST) as described in Standard Methods (APHA, 1998) . Sludge pH was measured with a pH meter (model 3200P, Agilent, Santa Clara, CA, USA). A UV spectrophotometer (UV-1800, Shimadzu, Kyoto, Japan) was used in the determination of proteins, carbohydrates and ammonia-nitrogen concentrations. Proteins concentration was determined with the method of Lowry et al. (1951) . Carbohydrates concentration was determined colorimetrically with the phenol-sulphuric acid method (DuBois et al., 1956) . Ammonia-nitrogen was measured colorimetrically using Nessler's reagent. VFAs concentration was analyzed with a gas chromatograph (7890A GC system, Agilent, Santa Clara, CA, USA) fitted with a flame ionization detector. The composition of biogas was measured with a gas chromatograph (7890A GC system, Agilent, Santa Clara, CA, USA) with thermal conductivity detectors.

Tian X., Trzcinski A.P., Lin L.L, & Ng W.J. (2015). Impact of ozone assisted ultrasonication pre-treatment on anaerobic digestibility of sewage sludge. Journal of environmental sciences (China), 33.

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Quantitative Formic Acid Analysis in Oxidized Samples

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GC measurements were performed to determine the emerging short chain formic acid content of the different formulations with advancing oxidation, as was previously described by Mahadevan et al. (1967) [63 (link)]. A 0.5 mL sample was diluted with 0.5 mL methanol and 0.1 mL sulfuric acid was added. The samples were sealed airtight and measured using a 7890A GC system with a polyethylene glycol column (Agilent Technologies, Inc., Santa Clara, CA, USA). Helium was used as carrier gas at 60 kPa. Measurements were conducted against formic acid calibration standards ranging from 20.16 to 504 µmol⋅mol⁻¹. The LOQ was 10 µmol⋅mol⁻¹ formic acid.

Kozuch B., Weber J., Buske J., Mäder K., Garidel P, & Diederichs T. (2023). Comparative Stability Study of Polysorbate 20 and Polysorbate 80 Related to Oxidative Degradation. Pharmaceutics, 15(9), 2332.

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Quantitative Analysis of HMF and DFF

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HMF and DFF were analyzed by HPLC
using a reversed-phase C18 column (250 × 4.6 mm) at 25 °C
with a detection wavelength of 280 nm. The mobile phase was acetonitrile
and 0.1 wt % acetic acid aqueous solution (65:35 v/v) at 0.5 mL/min.
The HMF conversion and DFF yield were expressed as mol % in terms
of the total HMF amount. The amounts of HMF and DFF in the samples
were calculated by interpolation from calibration curves. Calibration
curves for the observed products were constructed by injecting known
concentrations of reference commercial products. Other alcohols were
analyzed using GC (Agilent, GC-7890A) equipped with a flame ionization
detector and an Rtx-5 (30 m × 0.32 mm × 0.25 μm) capillary
column and further confirmed by GC–MS. GC–MS spectra
were performed using an Agilent Technologies 7890A GC system with
an Agilent 5975 inert mass selective detector (EI) and an HP-5 MS
column (0.25 mm × 30 m, film: 0.25 μm).

Hong M., Min J., Wu S., Cui H., Zhao Y., Li J, & Wang S. (2019). Metal Nitrate Catalysis for Selective Oxidation of 5-Hydroxymethylfurfural into 2,5-Diformylfuran under Oxygen Atmosphere. ACS Omega, 4(4), 7054-7060.

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Multimodal Analytical Techniques for Chemical Characterization

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GC-MS spectra were obtained with an HP 6890 GC (HP-5 MS column, 30 m) with an Agilent 5973 mass analyzer, an Agilent Technologies 7890AGC system, and 5975C VL MSD. Ammonia was used as the ionization gas for chemical ionization analysis. A PerkinElmer AutoSystem gas chromatograph (AT-1701 column, 30 m) with a flame ionization detector was used to record gas chromatograms. UV–vis spectra were recorded on a Hewlett-Packard (Agilent) 8453 diode-array spectrophotometer (190–1100 nm range) using a Unisoku cryostat cooled by liquid nitrogen. High-resolution mass spectra were obtained via an electrospray ionization–time-of-flight mass spectrometer. ³¹P NMR spectra were recorded on a VI-300 MHz instrument.

Shokri A, & Que L J.r. (2015). Conversion of Aldehyde to Alkane by a Peroxoiron(III) Complex: A Functional Model for the Cyanobacterial Aldehyde-Deformylating Oxygenase. Journal of the American Chemical Society, 137(24), 7686-7691.

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GC-MS Analysis of Essential Oils

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GC-MS analysis was performed on an Agilent 7890A GC system and an Agilent Technologies 5975C Inert XL Mass Selective Detector, equipped with an HP-5MS UI column (30 m×0.25 mm×0.25 μm; Agilent Technologies). Conditions were as follows: 5 µl sample injection, splitless injection, oven program 50 °C (1min hold) at 8 °C min^–1 to 300 °C (5min hold). For data processing, MSD ChemStation Data Analysis (Agilent Technologies) was used. The essential oil components were identified by comparison of their mass spectra with those in the NIST 2011 library data for GC-MS and comparison of their retention indices (RIs). The RIs were determined on the basis of an n-alkanes (C8–C40) mix standard (Sigma-Aldrich) under the same operation conditions. Camphor was added to serve as an internal standard. The amount of each compound was calculated by measuring its peak area related to that of a known amount of camphor. The identified components along with their RIs and relative percentage values are listed in Table 1.

Jin J., Kim M.J., Dhandapani S., Tjhang J.G., Yin J.L., Wong L., Sarojam R., Chua N.H, & Jang I.C. (2015). The floral transcriptome of ylang ylang (Cananga odorata var. fruticosa) uncovers biosynthetic pathways for volatile organic compounds and a multifunctional and novel sesquiterpene synthase. Journal of Experimental Botany, 66(13), 3959-3975.

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Analytical Methods for Chiral Compounds

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GC analyses were performed on a 7890A GC system (Agilent), equipped with an FID detector. HPLC analyses were carried on a 1100A HPLC system (Agilent), equipped with an VWD detector. The product yield and ee value of (R)-levodione were determined by GC using a CP-ChiraSil-DEX CB column (25 m × 0.25 mm × 0.25 μm, Agilent) as described previously.^{3,11 (link)} The product yield and de value of dihydrocarvone were determined by GC using a DB-5 column (30 m × 0.32 mm × 0.25 μm, Agilent) as described previously.^{3 (link)} The yield and ee value of 2-methyl-hydrocinnamaldehyde (12b) was determined by HPLC using a Chiralcel OJ-H column (250 mm × 4.6 mm, Daicel, Shanghai, China) as described previously.^{11 (link)} The conversion of citral (12a) was performed on GC using a HP-5 column (30 m × 0.32 mm × 0.25 μm, Agilent) as described previously.^{3 (link)} The ee value of citronellal was determined by GC using a Beta DEX 225 column (30 m × 0.25 mm × 0.25 μm, Supelco, Bellafonte, PA, USA) as described previously.^{3 (link)}

Zhang B., Sun J., Zheng Y., Mao X., Lin J, & Wei D. (2022). Identification of a novel ene reductase from Pichia angusta with potential application in (R)-levodione production. RSC Advances, 12(22), 13924-13931.

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GC Analysis of Fatty Acids

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Gas chromatography was performed using an 7890A GC System (Agilent Technologies, Palo Alto, CA, USA) equipped with a SUPELCOWAX™ 10 Capillary GC Column (15 mm × 0.10 mm, 0.10 μm); Supelco, Bellefonte, PA, USA). Chromatographic conditions were as follows: the initial temperature was 60 °C for 0 min; it increased at a rate of 40 °C/min to 160 °C (0 min); next, it increased at a rate of 30 °C/min to 190 °C (0.5 min), and next at a rate of 30 °C/min to 230 °C (2.6 min). The entire analysis took about 8 min, and the gas flow rate was 0.8 mL/min with hydrogen used as a carrier gas. Identification of fatty acids was done by comparing their retention times with those of commercially available standards.

Grzesiak K., Rył A., Stachowska E., Słojewski M., Rotter I., Ratajczak W., Sipak O., Piasecka M., Dołęgowska B, & Laszczyńska M. (2019). The Relationship between Eicosanoid Levels and Serum Levels of Metabolic and Hormonal Parameters Depending on the Presence of Metabolic Syndrome in Patients with Benign Prostatic Hyperplasia. International Journal of Environmental Research and Public Health, 16(6), 1006.

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Hydrocarbon Degradation Potential Assay

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The PHC degradation capability of the isolates that strongly exhibited (showing dark purple) degrading potential in colorimetric assays were further tested by inoculating them in Bushnell-Haas minimal medium amended with 1 mM concentrations of toluene or naphthalene using PTFE-lined screw capped tubes. The inoculated tubes were placed in incubated shaker at 27°C. After 1 week of incubation, residual hydrocarbons were extracted using hexane as extraction solvent following a method modified from Michaud et al. (2004 (link)). Toluene and naphthalene concentrations were determined using a Gas Chromatography-Flame Ionization Detector (Agilent Technologies 7890A GC System; using HP-5 30 m × 0.32 mm × 0.25 um column, helium carrier gas, hexane solvent) and compared to concentrations in uninoculated controls incubated and shaken for the same period of time.

Lumactud R., Shen S.Y., Lau M, & Fulthorpe R. (2016). Bacterial Endophytes Isolated from Plants in Natural Oil Seep Soils with Chronic Hydrocarbon Contamination. Frontiers in Microbiology, 7, 755.

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Quantifying Leaf Fructan Composition

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To determine the monosaccharide residue composition of the fructan, as well as to quantify the concentrations of fructans in leaves, acetylated alditol derivatization was performed [42 (link), 43 ]. This method hydrolyzes the polysaccharides to monosaccharides, which are then reduced with sodium borohydride (NaBH₄). The alditols were per-O-acetylated and extracted in ethyl acetate and 4 mL water. Fifty μL were taken for analysis by GC-MS (Agilent 7890A GC System with an automated sample injection, Agilent 7683 Automatic Liquid Sampler and Agilent 5975 MS). The column employed was a Supelco SP-2380 (Sigma-Aldrich, 30 m x 0.25 mm x 0.20 μm). GC / MSD ChemStation software version E.02.00.493 from Agilent Technologies was used to analyze the chromatograms.

Salinas C., Handford M., Pauly M., Dupree P, & Cardemil L. (2016). Structural Modifications of Fructans in Aloe barbadensis Miller (Aloe Vera) Grown under Water Stress. PLoS ONE, 11(7), e0159819.

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