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768 protocols using spss statistics 17

1

Statistical Data Analysis Protocol

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Statistical data analysis was performed using Microsoft Excel 2003 and SPSS Statistics 17.0 software. The mean, standard deviation and coefficient of variation were calculated by Microsoft Excel 2003. Drawing was performed using Origin 8.0 software. Image processing was performed using ERDAS IMAUINE 9.2 software, Adobe Photoshop CS6 and Image-Pro plus 6.0 software. The least significant difference (LSD) method was used for multiple comparisons by SPSS Statistics 17.0 software. The LSD test uses the square root of the residual mean square from the one-way analysis of variance (ANOVA) and considers it to be the pooled significant difference.
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2

Differential Expression and Virulence of P. palmivora

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For analyzing the differential expression of Ppal15kDa at various developmental stages and sporangium size measurements of P. palmivora WT and mutant strains, the one-way analysis of variance (ANOVA) according to Duncan’s multiple range tests was utilized to determine significance with P ≤ 0.05 and performed by using SPSS Statistics 17.0 software. The lesion area data derived from P. palmivora infection on N. benthamiana leaves with one half transiently expressing Ppal15kDaA or Ppal15kDaB compared to another half expressing GFP gene, and the lesion area data obtained from N. benthamiana leaves or papaya fruits with one half infected with WT P. palmivora compared to another half infected with mutant P. palmivora were analyzed by paired t-test (P-value ≤ 0.05) using SPSS Statistics 17.0 software.
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3

Kinetic and Weibull Analysis of Treatments

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All treatments were conducted in triplicate. The statistical significance of differences was evaluated by a one-way analysis of variance (ANOVA) and the Duncan post-hoc test (p = 0.05) using the SPSS statistics 17.0 software (SPSS Inc., Chicago, IL, USA). The parameters for the first-order kinetic and Weibull distribution models were obtained using the SPSS statistics 17.0.
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4

Malaria and HIV Data Collection Instrument

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A paper data collection instrument was developed, piloted and modified before use in the study. The instrument had three parts: (a) general information about the participants including socio-demographic variables and use of long-lasting insecticidal nets (LLINs); (b) clinical information (e.g. list of anti-malarial drugs used 28 days prior to data collection), laboratory data (e.g. level of haemoglobin, blood film result, parasite species and density) and the treatment given to the patient for a specific problem identified on the same date of diagnosis; and (c) detail of the medications taken by the HIV-positive patients and their CD4 count results.
Data was double entered using SPSS Statistics 17.0 (SPSS Inc., Chicago, USA). Any discrepancy between the two data sets was corrected by referring to the original paper form. The final single data set was cleaned and analyzed using STATA 11.0 (Statacorp, College Station, USA) and SPSS Statistics 17.0 (SPSS Inc., Chicago, USA). Data were organized and summarized using descriptive statistics, Chi square tests, and multivariate logistic regression; P values of <0.05 were considered statistically significant.
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5

Dose-response Analysis and Treatments Comparison

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The dose–response curve generated from the series 1 experiments was analysed using logistic regression (SPSS Statistics 17.0) to estimate the concentrations lethal to 10%, 50% and 99% of the population (LC10, LC50, LC99). For the series 2 experiments, two factor analysis of variance (ANOVA) was used to compare treatments (SPSS Statistics 17.0), using the data variables haemolymph osmolality, treatment and time (because these experiments were destructive, repeated measures ANOVA was inappropriate). Where there were significant interactions, multiple comparisons were done within levels of a factor using Tukey simultaneous tests (Minitab 15).
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6

Wolbachia Density Comparison in Mosquitoes

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To compare the densities of the two Wolbachia strains in field mosquitoes in five regions (with different adult sizes), data were normalized to the expression of the host rps6 gene. Analyses were carried out using SPSS Statistics (17.0). Chi-square tests were performed to compare the prevalence of Wolbachia infections and one-way analysis of variance (ANOVA) was performed to compare densities of Wolbachia from different regions for normally distributed data using SPSS Statistics (17.0). Differences were considered statistically significant when P < 0.05. For better presentation of results, locA and locB were used to denote the densities of supergroup A and supergroup B, respectively, from regions with local dengue cases; impA and impB were used to denote the densities of supergroup A and supergroup B, respectively, from regions with only imported dengue cases; and noA and noB were used to denote the densities of supergroup A and supergroup B, respectively, from regions with no dengue cases.
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7

Analyzing DNRA Dynamics and Drivers

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Pearson’s product correlation analysis was performed using SPSS statistics 17.0 package to explore the relationship among DNRA rate, nrfA gene abundance, the diversity index and dominant environmental parameters. Linear regression analyses between DNRA rates and nrfA gene abundances were conducted using Origin 9.0 and SPSS statistics 17.0.
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8

Oxidative Stress Responses in Turtles

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Variations in gene expression, enzyme activity, and lipid peroxidationquantification wereanalyzed acrossthe6groupsof turtles (control-12h, control-96h, glyphosate-12h, glyphosate96h, Fosseille-12h, and Fosseille-96h) for each measure (SOD gene expression, SOD enzyme activity, CAT gene expression, CAT enzyme activity, AChE enzyme activity, and lipid peroxidation quantification) following the nonparametric Kruskal-Wallis test using SPSS Statistics 17.0 (SPSS). Pairwise comparisons for each measure were subsequently done following the nonparametric Mann-Whitney U test using SPSS Statistics 17.0. Statistical significance was accepted at p<0.05.
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9

Nitrogen Management Strategies for Optimal Crop Yield

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In this study, 2-year data of yield and net assimilation rate were presented, and the average of 2-year data was used for other indicators. Microsoft Excel 2019 was used to organize, calculate, and chart the data. IBM SPSS Statistics 17.0 (IBM Inc., Chicago, IL, USA) was used for statistical analysis. Analysis of variance was performed with IBM SPSS Statistics 17.0 (IBM Inc., Chicago, IL, USA) to test the effects of year, treatments (CN and PN), and N rates on grain yield, agronomic traits, N content, and nitrogen use efficiency. The DUNCAN method and paired t-test (p < 0.05) were used to determine the differences among the treatments.
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10

Effect of Treatment on Biological Outcomes

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The data have been reported as mean ± standard deviation (S.D.) from three independently performed experiments. Statistical analysis involving three or more groups was conducted using One-way ANOVA with SPSS Statistics 17.0 (IBM Corporations, New York, NY, USA), with significance set at p < 0.05. Conversely, statistical analysis for before and after treatments was determined by the paired t-test using SPSS Statistics 17.0 (IBM Corporations, New York, NY, USA), with significance levels denoted as * p < 0.05, ** p < 0.01, and *** p < 0.001.
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