Next, the extracts were dissolved in 1 ml hexane for analysis by gas chromatography and mass spectrometry (GC–MS). For analysis, a 1 μL sample was injected into an Agilent 7000 gas chromatograph in a nonshunt manner using a DB-5 ms capillary column (injector temperature, 305°C) with a DB-5 ms (30 m × 250 μm, film thickness 0.1 μm) capillary column. One microlitre of the concentrated organic phase was then injected at a He flow rate of 1 ml/min with a temperature program of 1 min at 50°C, followed by a gradient from 50 to 260°C at 50°C/min and then to 305°C at 20°C/min, with a 15 min hold. The ion trap temperature was 230°C. The electron energy was 70 eV. Spectra were recorded in the range of 10–550 m/z. Standard chemicals were purchased from YuanYe Biotech (Shanghai, China).
Db 5ms
Manufactured by Agilent Technologies
Sourced in United States, Japan, Germany, China
The DB-5MS is a gas chromatography (GC) column manufactured by Agilent Technologies. It is a capillary column designed for the separation and analysis of a wide range of organic compounds. The DB-5MS column features a 5% phenyl-methylpolysiloxane stationary phase, which provides good thermal stability and inertness for a variety of applications.
Automatically generated - may contain errors
Lab products found in correlation
Gcms qp2010, by Shimadzu (9 mentions)
5973 mass selective detector, by Agilent Technologies (6 mentions)
Agilent 6890n, by Agilent Technologies (4 mentions)
7890a gc system, by Agilent Technologies (4 mentions)
Agilent 7890b, by Agilent Technologies (4 mentions)
Gc ms, by Agilent Technologies (4 mentions)
5975c mass spectrometer, by Agilent Technologies (3 mentions)
Msd 5973, by Agilent Technologies (3 mentions)
Agilent 6890 gc, by Agilent Technologies (3 mentions)
Formic acid, by Thermo Fisher Scientific (3 mentions)
Triplus rsh autosampler, by Thermo Fisher Scientific (3 mentions)
Model 6890 instrument, by PerkinElmer (2 mentions)
Q mass 910 quadrupole selective detector, by PerkinElmer (2 mentions)
Trace gc ultra apparatus, by Thermo Fisher Scientific (2 mentions)
Enhanced chemstation software, by Agilent Technologies (2 mentions)
Agilent 7683, by Agilent Technologies (2 mentions)
Gc 17a, by Shimadzu (2 mentions)
5975c vl msd, by Agilent Technologies (2 mentions)
7890a gas chromatograph, by Agilent Technologies (2 mentions)
Db 17ht, by Agilent Technologies (2 mentions)
222 protocols using db 5ms
1
GC-MS Analysis of Organic Extracts
2
GC–TOF–MS Analysis of Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC–TOF–MS analysis was performed using an Agilent 7890 gas chromatograph (7890A, Agilent) coupled with a time-of-flight mass spectrometer (PEGASUS HT, LECO, Beijing, China). The system utilized a DB-5MS capillary column (DB-5MS (30 m × 250 μm × 0.25 μm), Agilent, Santa Clara, CA, USA). A 1 μL aliquot of each sample was injected in splitless mode. Helium was used as the carrier gas, the front inlet purge flow was 3 mL/min, and the gas flow rate through the column was 1 mL/min. The initial temperature was maintained at 50 °C for 1 min, raised to 310 °C at a rate of 10 °C/min, and then maintained for 8 min at 310 °C. The injection, transfer line, and ion source temperatures were 280, 280, and 250 °C, respectively. The energy was −70 eV in the electron impact mode. Mass spectrometry data were acquired in the full scan mode with an m/z range of 50–500 at a rate of 12.5 spectra per second after a solvent delay of 6.35 min.
3
GC-MS Analysis of Cyclia oppositifolia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The GC-MS analysis of n-hexane fractions of leaves stems and inflorescence of C. oppositifolia, was performed using Agilent GC-MS, equipped with DB-5 MS split and split-less mode column model DB-5 MS dimensions (30 nm X 0.25 mm), the diameter of 0.25 μm. The operation mode was conducted at 70 eV. Helium was the carrier gas maintained at a pressure of 11.66 psi and a flow rate of 1.00 mL/min. The injector was operated in the temperature range of 45-350 °C. The oven temperature was programmed to increase as follows; 50 °C at 6 °C/min to 200 °C (5 min) at 6 °C/min to 325 °C (10 min). The temperature was kept constant for 5 min at the beginning of the procedure and the end of the sample run. The sample solution was prepared in the analytical grade of n-Hexane, filtered through 0.45 μm filter using filtration syringe. The analysis was carried out utilizing split-less mode, injecting 2.00 μL of the analyte sample at 50 °C (Peter et al. 2012) .
A mass range of 35-500 atomic mass unit (amu) was scanned and analyzed with the help of GC-MS lab-solution software that contained in it NIST-417 LIB, for identification and characterization of a sample. The name, molecular formula of the components was ascertained and by using homologous series of compounds the retention indices for each compound were assessed.
A mass range of 35-500 atomic mass unit (amu) was scanned and analyzed with the help of GC-MS lab-solution software that contained in it NIST-417 LIB, for identification and characterization of a sample. The name, molecular formula of the components was ascertained and by using homologous series of compounds the retention indices for each compound were assessed.
4
GC-MS Analysis of Intracellular Metabolites
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The derivatized intracellular metabolite samples were analyzed using a Bruker 450-GC instrument coupled with the Bruker 300-MS single quadruple mass spectrometer (Bruker, Inc. Billerica, MA, USA). The system used a silica capillary column DB-5ms (30 m × 0.25 mm ID, 0.25-μm film thickness, J&W Scientific, Folsom, CA, USA). Helium (99.999% purity) was used as a carrier gas, at a flow rate of 1.0 ml min−1. The column oven temperature was initially kept at 70 °C for 1 min, increased to 80 °C at a rate of 2 °C min−1 and maintained for 1 min, increased to 200 °C at a rate of 6 °C min−1 and maintained for 3 min, and then increased to 270 °C at a rate of 15 °C min−1 and maintained for 5 min. The temperatures for the front inlet, transfer line, and ion source were 270, 250, and 200 °C, respectively. The injected volume for each sample was 1 μl in a 1:15 split ratio mode. The mass spectrometry data were acquired in the full-scan mode over an m/z range of 50–800 for metabolite identification and single-ion monitoring mode for isotopomer analysis after a solvent delay of 7 min.
5
GC/MS Analysis of Antifungal Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC/MS analyses were carried out at the Seoul Center at the Korea Basic Science Institute. The antifungal fractions from semi-preparative TLC were placed in an Agilent GC/MS system equipped with a J&W DB-5ms capillary column. Analysis conditions were as follows: injector, split ratio (2:1); injection temperature, 250 °C; injection volume, 1 mL; carrier gas, He (1.0 mL/min); column, DB5-MS J&W Scientific (Folsom, CA) ; oven temperature, from 60 °C (2 min) to 320 °C at intervals of 10 °C/min; interface temperature, 280 °C; ion source, EI, 230 °C; analyzer, quadrupole, 150 °C; mass range, 40–800 m/z.
6
GC-MS Analysis of Insect Pheromone Compounds
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Female extracts and synthetic standards were analyzed on an Agilent 6890 N GC interfaced to an Agilent 5975 C mass-selective detector as previously described40 (link). Samples were run on a non-polar DB-5MS or polar DB-Waxetr column (30 m×0.25 mm ID, 0.25 μm film thickness; J&W Scientific, CA, USA). For the DB-5MS column, the oven temperature was identical to that previously described for the GC-EAD system. For the DB-Waxetr column, the oven temperature was maintained at 80 °C for 1 min, increased to 180 °C at 10 °C/min, then to 220 °C at 5 °C/min, and held for 10 min. The injection was splitless, and helium was the carrier gas (1 ml/min). The injector and transfer line temperatures were 250 °C. Electron ionization mass spectra were recorded from m/z 30 to 350 at 70 eV, with the ion source temperature of 230 °C. GC retention times are quoted as retention indices (RIs) relative to those of n-alkanes. Compounds from female thoracic extracts were identified by comparisons of their RIs and mass spectra with those of authentic standards on the DB-5MS and DB-Waxetr columns. Quantities of each compound in the female extracts were calculated using hexane solution (1 ng/uL) of synthetic racemic 2,10-DiMe-12Pr as an external standard.
7
GC-MS Analysis of Complex Samples
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The given extract was analyzed by GC–MS electron impact ionization (EI) method on GC-17A gas chromatograph (Shimadzu) coupled to a GC–MS QP 5050A Mass Spectrometer (Shimadzu). A fused silica capillary column (30 m × 2.5 mm; 0.25 mm film thickness), coated with DB-5 ms (J&W) was used. The following conditions were used for GC–MS run; Injection temperature: 300 °C, interface temperature: 300 °C, ion source was adjusted to 250 °C, carrier gas: helium (flow rate of 1 ml min−1). The analysis was performed following temperature program: 1 min. of isothermal heating at 100 °C followed by heating at 300 °C for 20 min. The mass spectra were recorded at 2 scan sec-1 with a scanning range of 40–850 m/z. Each component was quantified based on peak areas and normalization based on the internal standard.
8
GC-MS and GC-FID Analysis Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC/MS determinations were carried out in a Hewlett Packard model 6890 instrument coupled to a Q-Mass 910 quadrupole selective detector (Perkin-Elmer) at 70 eV. A fused capillary column was used (DB-5MS, 30 m × 0.25 mm i.d.; film thickness 0.25 μm; J&W Scientific); injection port temperature, 230°C; splitless for 1 min then split ratio 1/10; detector temperature, 270°C; carrier gas, helium at 0.7 mL/minute; temperature program: 50–230°C linear increase at 3 °C/minute. Scanning speed was 2.48 scan/second with mass spectra recorded from 50 to 650 m/z. GC/FID determinations were performed on a Trace GC Ultra apparatus (Thermo Electron Corporation) equipped with an FID. The output was recorded using a ChromQuest version 4.1 data system. Analyses were performed on capillary columns DB-5MS at the conditions stated above (Adams, 2007 ; Araujo et al., 2007 (link); Babushok et al., 2011 (link)).
9
GC-MS Analysis of Fatty Acid Derivatives
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The FAME extracts and respective DMOX and TMS derivatives were analyzed with a 7890A gas chromatograph coupled to a 5975C mass spectrometer, equipped with electron ionisation and quadruple analyser (Agilent Technologies, Santa Clara, CA, USA) using DB-5MS or DB-WAX capillary columns (both J&W Scientific, Folsom, CA, USA; 30 m×0.25 mm, film thickness 0.25 mm); the electron ionisation was set at 70 eV.
Conditions for the analysis of FAMEs and DMOX derivatives were as follows: carrier gas, He: 1 ml/min; 10∶1 split ratio, injection volume 2 μl; injector temperature 220°C; thermal gradient 140°C to 245°C at 3°C/min, then at 8°C/min to 280°C and temperature held for 5 min. The temperature program was terminated at 245°C and held at this temperature for 10 min when the DB-WAX column was used. The TMS derivatives were analyzed on a DB-5MS column using the following thermal gradient: 50°C to 140°C at 10°C/min, then 1°C/min to 198°C, 3°C/min to 320°C and temperature held for 3 min.
Conditions for the analysis of FAMEs and DMOX derivatives were as follows: carrier gas, He: 1 ml/min; 10∶1 split ratio, injection volume 2 μl; injector temperature 220°C; thermal gradient 140°C to 245°C at 3°C/min, then at 8°C/min to 280°C and temperature held for 5 min. The temperature program was terminated at 245°C and held at this temperature for 10 min when the DB-WAX column was used. The TMS derivatives were analyzed on a DB-5MS column using the following thermal gradient: 50°C to 140°C at 10°C/min, then 1°C/min to 198°C, 3°C/min to 320°C and temperature held for 3 min.
10
Organic Biomarker Analysis in Sediments
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
For the organic biomarker analysis, freeze-dried sediment samples were Soxhlet extracted for 72 h with 2:1 dichloromethane/methanol. The extracts were concentrated using rotary evaporation and then saponified using 0.5 M KOH/MeOH. The neutral lipids were partitioned out of the basic solution with hexane and further separated using (5% deactivated) silica gel column chromatography and solvents of increasing polarity from hexane to methylene chloride. The alkane fraction was eluted and obtained in the hexane solvent.
This alkane fraction was analysed in an HP 5890 gas chromatograph-mass spectrometer with a DB-5 ms (50 m × 0.32 mm and 0.25 μm film thickness) capillary column (J&W). Helium was used as the carrier gas. The mass spectrometer was operated in EI mode at 70 eV. The MS data were acquired in the full mode and processed using the Chemstation data system. The GC oven for alkanes was programmed as follows: held for 2 min at 60°C, increased to 200°C at 7°C/min, then increased to 280°C at 3°C/min, and finally held for 30 min at 280°C.
This alkane fraction was analysed in an HP 5890 gas chromatograph-mass spectrometer with a DB-5 ms (50 m × 0.32 mm and 0.25 μm film thickness) capillary column (J&W). Helium was used as the carrier gas. The mass spectrometer was operated in EI mode at 70 eV. The MS data were acquired in the full mode and processed using the Chemstation data system. The GC oven for alkanes was programmed as follows: held for 2 min at 60°C, increased to 200°C at 7°C/min, then increased to 280°C at 3°C/min, and finally held for 30 min at 280°C.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!