Facsariaii sorter

Manufactured by BD

Sourced in United States

The FACSAriaII is a high-performance cell sorter designed for precise and efficient cell analysis and separation. Its core function is to rapidly identify, isolate, and collect specific cell populations from complex samples based on their unique physical and fluorescent characteristics.

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Lab products found in correlation

Cd19 fitc, by BD (1 mentions) Cd3 pacific blue, by BD (1 mentions) Cd20 pe cy7, by BD (1 mentions) Cd27 apc, by Thermo Fisher Scientific (1 mentions) Cd38 pe, by BD (1 mentions) Lsrfortessa, by BD (1 mentions) Facscalibur, by BD (1 mentions) Moflo astrios cell sorter, by Beckman Coulter (1 mentions)

Close spelling variants of this product

BD FACSAriaII cell sorters (3 citations) FACSAriaII High-Speed Cell Sorter (4 citations) FACSAriaⅡ (12 citations)

3 protocols using facsariaii sorter

Phenotyping and Sorting Plasmablasts

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PBMCs obtained from the two patients were stained with titrated amounts of CD19-FITC (BD; clone HIB19), CD3-Pacific Blue (BD; clone SP34-2), CD20-PECy7 (BD; clone L27), CD27-APC (eBiosciences; clone O323) and CD38-PE (BD; clone HIT2). The plasmablast population was defined as CD3− CD19+ CD20−/low CD27+ CD38+ lymphocytes and its frequency analyzed using FlowJo software. The single-cell sorting of plasmablasts was carried out using the FACSAriaII sorter (Becton Dickinson; Franklin Lakes, NJ, USA) at the Emory University Pediatrics Flow Cytometry Core Facility under negative air pressure. The cells were sorted into a 96-well PCR plate as described in [34 (link),35 (link)], rapidly frozen on dry ice and stored at −80 °C for subsequent cDNA synthesis.

Bhaumik S.K., Priyamvada L., Kauffman R.C., Lai L., Natrajan M.S., Cho A., Rouphael N., Suthar M.S., Mulligan M.J, & Wrammert J. (2018). Pre-Existing Dengue Immunity Drives a DENV-Biased Plasmablast Response in ZIKV-Infected Patient. Viruses, 11(1), 19.

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Multiparametric T Cell Profiling

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Samples were acquired on a FACSCalibur or LSR Fortessa flow cytometer (Becton Dickinson, Mountain View, CA), and data were analyzed using FlowJo software (Tree Star Inc., Ashland, OR). Cells were sorted either on FACS Aria II sorter (Becton Dickinson, Mountain View, CA) or MoFlo Astrios cell sorter (Beckman Coulter, Miami, FL). Detailed protocols for Activation induced T cell death (AICD), staining the cells for mitochondrial membrane potential (DiOC6), reactive oxygen species (ROS), reactive nitrogen species (RNS), iGSH, and glucose consumption assay (2NBDG), has been described in supplementary methods.

Kesarwani P., Al-Khami A.A., Scurti G., Thyagarajan K., Kaur N., Husain S., Fang Q., Naga O.S., Simms P., Beeson G., Voelkel-Johnson C., Garrett-Mayer E., Beeson C.C., Nishimura M.I, & Mehrotra S. (2014). Promoting Thiol Expression Increases The Durability of Antitumor T cell Functions. Cancer research, 74(21), 6036-6047.

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Isolation and FACS Sorting of Lgr5+ Intestinal Stem Cells

Cited 1 time

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Intestines harvested from Lgr5^GFP mice (Barker et al., 2007 (link)) were washed with phosphate-buffered saline (PBS). Villi were scraped away using cover slips and the crypt epithelium collected by shaking in 5 mM EDTA for 1 h at 4°C (Kim et al., 2014 (link)). Single cells were obtained on 2 separate days by digestion in 5X TrypLE (Invitrogen) for 1 h at 37°C and verified by fluorescence microscopy. GFP^hi cells were sorted into individual wells in 96-well plates using a FACSAria II sorter (Becton-Dickinson). Cells from one of the two isolations were also examined visually in microfluidic channels.

Kim T.H., Saadatpour A., Guo G., Saxena M., Cavazza A., Desai N., Jadhav U., Jiang L., Rivera M.N., Orkin S.H., Yuan G.C, & Shivdasani R.A. (2016). Single-cell transcript profiles reveal multilineage priming in early progenitors derived from Lgr5+ intestinal stem cells. Cell reports, 16(8), 2053-2060.

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