The human breast cancer MDA-MB-231 cell line was purchased from ATCC (ATCC; Rockville, MD, USA). MDA-MB-231 cells were cultured in DMEM High-Glucose medium (Beyotime, Nanjing, China) containing 10% fetal bovine serum (FBS, Hyclone, UT, USA) and antibiotics (100 IU/mL penicillin and 100 μg/mL streptomycin, Beyotime, Nanjing, China) at 37 °C in a humidified 5% CO2 atmosphere. The cells were routinely cultured in cells Petri dishes (Beyotime, Nanjing, China) or were seeded in 96-well plates (Beyotime, Nanjing, China).
Mda mb 231
Manufactured by Beyotime
Sourced in China
MDA-MB-231 is a human breast cancer cell line. It is commonly used in cancer research and drug development studies.
Automatically generated - may contain errors
Lab products found in correlation
96 well plate, by Beyotime (1 mentions)
Mda mb 231 cell line, by American Type Culture Collection (1 mentions)
Dmem high glucose medium, by Beyotime (1 mentions)
Penicillin, by Beyotime (1 mentions)
Streptomycin, by Beyotime (1 mentions)
Foetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (1 mentions)
Cck 8 kit, by Beyotime (1 mentions)
Mda mb 231, by National Collection of Authenticated Cell Cultures (1 mentions)
3 protocols using mda mb 231
1
Culturing MDA-MB-231 Breast Cancer Cells
2
Comparative Cultivation of Breast Cell Lines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The normal breast epithelial cell line MCF10A, the human BC cell lines MCF-7, SKBR-3, and MDA-MB-453, and the Chinese Academy of Sciences’ Cell Bank of Type Culture Collection. DMEM (Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% (v/v) foetal bovine serum (Gibco; Thermo Fisher Scientific, Inc.) and a 1% (v/v) penicillin and streptomycin solution was employed to regularly cultivate SKBR-3, MCF-7, and MDA-MB-231 cells (Beyotime Institute of Biotechnology). A mammary epithelial cell environment (Procell Life Science & Technology Co., Ltd.) containing 10% horse serum, EGF, hydrocortisone, insulin, and 1% penicillin-streptomycin was employed to cultivate MCF10A cells. All cell lines were perfused employing conventional cell culture methods and grown in an incubator at 37°C with 5% CO2.
3
Cytotoxicity Evaluation of Novel Nanomedicines
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
MDA-MB-231 cell lines were purchased from the Cell Bank of the Chinese Academy of Sciences (Shanghai, China). MDA-MB-231 cells were cultured with Dulbecco's modified Eagle's medium (DMEM) containing 10% FBS, and 1% penicillin-streptomycin with 5% CO2 at 37 °C.
Cell cytotoxicity assay was employed using CCK-8 kit (Beyotime). Briefly, the cells were firstly implanted in 24-well plates at a density of 2 × 104/well. When the cell density reached to 60–70%, HPPPH, LND (15 μg/mL), HPPPH@L (65.2 μg/mL) and HPPPH@L@B (65.2 μg/mL, as same dosage of LND) were added and co-incubated with MDA-MB-231 cell for 24 h or 48 h. Subsequently, the cells were washed with PBS, followed by co-culturing with 200 μL fresh medium and 20 μL CCK-8 solution at 37 °C for 1.5 h. Afterwards, the supernatant was transferred to a 96-well plate and measured with a microplate reader (Tecan Spark, Tecan) at 450 nm.
Cell cytotoxicity assay was employed using CCK-8 kit (Beyotime). Briefly, the cells were firstly implanted in 24-well plates at a density of 2 × 104/well. When the cell density reached to 60–70%, HPPPH, LND (15 μg/mL), HPPPH@L (65.2 μg/mL) and HPPPH@L@B (65.2 μg/mL, as same dosage of LND) were added and co-incubated with MDA-MB-231 cell for 24 h or 48 h. Subsequently, the cells were washed with PBS, followed by co-culturing with 200 μL fresh medium and 20 μL CCK-8 solution at 37 °C for 1.5 h. Afterwards, the supernatant was transferred to a 96-well plate and measured with a microplate reader (Tecan Spark, Tecan) at 450 nm.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!