Pm1 microplate

Manufactured by Biolog

Sourced in United States

The PM1 MicroPlate is a lab equipment product used for the identification and characterization of microorganisms. It provides a standardized format for testing the utilization of 95 different carbon sources by a microbial culture.

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Lab products found in correlation

Omnilog pm m system, by Biolog (1 mentions) Digitonin, by GoldBio (1 mentions) If oa buffer, by Biolog (1 mentions) Redox dye mix a, by Biolog (1 mentions) Infinite m200 plate reader, by Tecan (1 mentions) Trypticase soy agar plate, by BD (1 mentions)

Spelling variants of this product

PM1 and PM2A microplates (2 citations)

3 protocols using pm1 microplate

Mitochondrial Metabolic Activity Assay

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Mitochondrial metabolic activity was measured in digitonin-permeabilized cells using the PM1 MicroPlate (Biolog #12111, Hayward, CA, USA) following the manufacturer’s instructions (protocol document dated February 6, 2019 using cell preparation protocol Option 1). Briefly, cells were grown in lactate medium, washed and resuspended in high lactose osmotic stabilizing solution (YMAS), containing digitonin (#D-180-250, Gold Biotechnology, USA). After 1 hr, the permeabilized cells were seeded into 96-well PM1 MicroPlates containing different potential energy substrates. The colorimetric assay was initiated by adding Redox Dye Mix MC (Biolog #74353). The PM1 MicroPlate was then loaded into the OmniLog PM-M system (Biolog, Hayward, CA, USA) for kinetic reading at 30°C. The MitoPlates were read for 24 hr at 15 min intervals at OD590. OmniLog rate values were calculated using Data Analysis 1.7 software before being transferred to a Microsoft Excel sheet for reformatting. The software calculates the maximal rate change from the gradient of the reading output during the 24 hr time period. These data are presented as raw OD590 (A.U.).

Farre J.C., Carolino K., Devanneaux L, & Subramani S. (2022). OXPHOS deficiencies affect peroxisome proliferation by downregulating genes controlled by the SNF1 signaling pathway. eLife, 11, e75143.

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Carbon Source Phenotyping of Bacteria

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For Carbon Source Phenotype Microarray™ (Biolog) assays, a stock solution of 2 × 10⁷ bacteria/ml was prepared in 1X IF-Oa buffer (Biolog, 72268) supplemented with 1X Redox Dye Mix A (Biolog, 74221). 100 μl of this stock solution (delivering 2 × 10⁶ bacteria) was added to each well of a PM1 Microplate™ (Biolog, 12111) and the plate was incubated at 37°C overnight with shaking. Absorbance was read at 590 nm on the infinite M200 plate reader (TECAN).

Wong Fok Lung T., Charytonowicz D., Beaumont K.G., Shah S.S., Sridhar S.H., Gorrie C.L., Mu A., Hofstaedter C.E., Varisco D., McConville T.H., Drikic M., Fowler B., Urso A., Shi W., Fucich D., Annavajhala M.K., Khan I.N., Oussenko I., Francoeur N., Smith M.L., Stockwell B.R., Lewis I.A., Hachani A., Baskota S.U., Uhlemann A.C., Ahn D., Ernst R.K., Howden B.P., Sebra R, & Prince A. (2022). Klebsiella pneumoniae induces host metabolic stress that promotes tolerance to pulmonary infection. Cell metabolism, 34(5), 761-774.e9.

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Microbial Carbon Substrate Profiling

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Normal saline was inoculated with 24-h cultures grown on Trypticase soy agar plates (BD) (30 g/liter Trypticase soy broth, 15 g/liter agar, where soy broth components included 17 g/liter pancreatic digest of casein, 3 g/liter papaic digest of soybean meal, 5 g/liter NaCl, 2.5 g/liter K₂PO₄, and 2.5 g/liter dextrose) from which 150 µl of the inoculated saline at 53% to 59% turbidity was transferred into each of the 96 wells (each containing a different carbon substrate) of an ES MicroPlate plate (Biolog; now available as PM-1 MicroPlate), and substrate utilization was observed after 24 h at 37°C.

Choe E., Kazmierczak R.A, & Eisenstark A. (2014). Phenotypic Evolution of Therapeutic Salmonella enterica Serovar Typhimurium after Invasion of TRAMP Mouse Prostate Tumor. mBio, 5(4), e01182-14.

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