Moflo astrios eq

Manufactured by Beckman Coulter

Sourced in United States, Japan

The MoFlo Astrios EQ is a high-performance flow cytometry system designed for advanced cell sorting applications. It features a robust and reliable architecture to deliver consistent and accurate results. The core function of the MoFlo Astrios EQ is to provide users with a powerful tool for the precise separation and isolation of diverse cell populations from complex samples.

Automatically generated - may contain errors

Lab products found in correlation

Close spelling variants of this product

MoFlo Astrios (315 citations) MoFlo Astrios EQ cell sorter (48 citations) MoFlo Astrios EQ flow cytometer (19 citations) Astrios EQ (15 citations) MoFlo Astrios EQ sorter (9 citations) Beckman moflo Astrios EQ (7 citations) MoFlo Astrios EQ cytometer (5 citations) MoFlo Astrios EQ high speed cell sorter (5 citations) MoFlo Astrio EQ cell sorter (3 citations) MoFlo Astrios EQ device (3 citations)

238 protocols using moflo astrios eq

Cell Cycle and Apoptosis Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were centrifuged at room temperature and 1,000 × g for 1 min and collected following trypsin detachment, and 2 μl of 1 mg/ml RNase A (prepared by deionized water) were added at 37°C for 40 min to remove the RNA. Cells were treated with propidium iodide (PI) staining solution and incubated at room temperature without exposure to light for 20 min. Finally, the cell cycle distribution was detected using a flow cytometer (MoFloAstrios EQ, Beckman Coulter, Inc.).
The cells were centrifuged and collected following trypsin detachment. To each tube, 100 μl 1X Annexin binding buffer was added to resuspend the cell precipitates. Alexa Fluor 488 Annexin V-fluorescein isothiocyanate (FITC) and 1 μl of 100 mg/ml PI staining solution were mixed and added to the cells, followed by incubation at room temperature for 15 min without light exposure. Apoptotic cells were measured with a flow cytometer (MoFloAstrios EQ, Beckman Coulter, Inc.).

Huang J., Xiao R., Wang X., Khadka B., Fang Z., Yu M., Zhang L., Wu J, & Liu J. (2021). MicroRNA-93 knockdown inhibits acute myeloid leukemia cell growth via inactivating the PI3K/AKT pathway by upregulating DAB2. International Journal of Oncology, 59(4), 81.

+ Open protocol

+ Expand

Isolation and Co-culture of MAIT and B Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

CD19⁺ and CD14⁺ cells were deleted from splenocytes from Vα19iTg Cd1d1^−/− mice using anti-mouse CD19 and anti-CD14 MicroBeads (Miltenyi Biotec). MAIT cells (F4/80⁻ CD3⁺ MR1⁻tetramer⁺) were sorted from CD14⁻CD19⁻ splenocytes by MoFlo Astrios EQ (Beckman Coulter). CD19⁺ splenocytes were sorted from the spleens of FcγRIIb^−/−Yaa mice using anti-mouse CD19 MicroBeads. Then, 100,000 or 20,000 MAIT cells and 20,000 CD19⁺ cells were co-cultured in 96-well V-bottom plates (Corning) with 100 ng/mL of lipopolysaccharide (LPS; InvivoGen) for 7 days. In some experiments, 10 μg/mL of anti-mouse CD154 antibody, anti-mouse CD275 antibody, anti-mouse MR1 antibody (all from BioLegend) or 10 μM i6-FP was added.

Murayama G., Chiba A., Suzuki H., Nomura A., Mizuno T., Kuga T., Nakamura S., Amano H., Hirose S., Yamaji K., Suzuki Y., Tamura N, & Miyake S. (2019). A Critical Role for Mucosal-Associated Invariant T Cells as Regulators and Therapeutic Targets in Systemic Lupus Erythematosus. Frontiers in Immunology, 10, 2681.

+ Open protocol

+ Expand

Isolation and Imaging of Blood Cell-Derived EVs

Check if the same lab product or an alternative is used in the 5 most similar protocols

To confirm the association of blood cells with EVs, monocytes, granulocytes, as well as T cells were sorted onto tissue culture slides as described below, fixed, and analyzed by high resolution confocal microscopy. Human whole blood was stained for 15 min with lactadherin-AF647, CD45-PB, and CD41-AF488, and either CD14-PE for the sorting of monocytes, CD66b-PE for the sorting of granulocytes, and CD3-PE for the sorting of T cells. Stained blood was diluted 1:10 with PBS, and cell sorting was performed using a MoFloAstrios EQ (Beckman Coulter). To exclude red blood cells and platelets, cells were depicted on a dot plot using side scatter (SS) against CD45. CD45⁺ cells were gated and divided into a CD45⁺/CD14⁺ plot, a CD45⁺/CD66b⁺ plot and a CD45⁺/CD3⁺ plot. After color compensation, CD45⁺CD14⁺ monocytes, CD45⁺CD66b⁺ granulocytes, and CD45⁺CD3⁺ T cells were sorted onto 18-well flat bottom µ-slides treated for tissue culture (ibidi, Martinsried, Germany). Just before sorting, side streams were adjusted to fit exactly into the wells. Wells were prefilled with fixation solution containing 7.5% formaldehyde (Liquid Productions GmbH, Flintsbach, Germany) to prepare cells for high resolution imaging. In total, 5,000 cells of each population were sorted in purity mode (n = 5).

Weiss R., Gröger M., Rauscher S., Fendl B., Eichhorn T., Fischer M.B., Spittler A, & Weber V. (2018). Differential Interaction of Platelet-Derived Extracellular Vesicles with Leukocyte Subsets in Human Whole Blood. Scientific Reports, 8, 6598.

+ Open protocol

+ Expand

Single-Cell Isolation from Tumor Tissue

Check if the same lab product or an alternative is used in the 5 most similar protocols

Following resection, a random piece was selected from viable tumor tissue, minced, and viably frozen. On the day of the sorting, the sample was thawed and dissociated into a single-cell suspension in AdDF + ++ (Advanced DMEM/F12 containing 1× Glutamax, 10 mM HEPES and antibiotics) containing Collagenase 1a (1 mg/mL, Sigma, C9407) and DNase (0.25 µg/mL, Stemcell), supplemented with Rho-kinase inhibitor Y-27632 (10 µM, Abmole). The samples were digested on an orbital shaker for 30 min at 37 °C. The suspension was washed first with AdDF + ++ and next with MACS buffer (PBS pH 7.2 + 2 mM EDTA + 0.5% Bovine Serum Albumine), followed each time by centrifugation at 300 × g. Viable single cells were sorted based on forward/side scatter properties and DAPI/DRAQ5 staining using FACS (MoFlo Astrios EQ, Beckman Coulter) into 384-well plates (Biorad) containing 10 µl mineral oil (Sigma) and 50 nl of RT primers.

Young M.D., Mitchell T.J., Custers L., Margaritis T., Morales-Rodriguez F., Kwakwa K., Khabirova E., Kildisiute G., Oliver T.R., de Krijger R.R., van den Heuvel-Eibrink M.M., Comitani F., Piapi A., Bugallo-Blanco E., Thevanesan C., Burke C., Prigmore E., Ambridge K., Roberts K., Braga F.A., Coorens T.H., Del Valle I., Wilbrey-Clark A., Mamanova L., Stewart G.D., Gnanapragasam V.J., Rampling D., Sebire N., Coleman N., Hook L., Warren A., Haniffa M., Kool M., Pfister S.M., Achermann J.C., He X., Barker R.A., Shlien A., Bayraktar O.A., Teichmann S.A., Holstege F.C., Meyer K.B., Drost J., Straathof K, & Behjati S. (2021). Single cell derived mRNA signals across human kidney tumors. Nature Communications, 12, 3896.

+ Open protocol

+ Expand

Cell Cycle Analysis of E. tenella Infection

Check if the same lab product or an alternative is used in the 5 most similar protocols

Outbred PA12 chickens aged 4 to 6 weeks were infected orally with 7.5 × 10⁵ sporulated oocysts of the recombinant

E. tenella

mCherry strain. Chickens were euthanised at 84 hr p.i., and caeca were recovered. Caecal tissues were incubated with collagenase H (1 mg/ml) at 250 rpm, at 37°C for 30 min. Infected and noninfected epithelial cell from the caecum were stained for cell cycle analysis. Then mCherry‐positive cells (i.e., infected cells) and mCherry‐negative cells (i.e., noninfected cells) were separately sorted by flow cytometry on a MoFlo Astrios EQ (Beckman Coulter) high‐speed cell sorter.

Diallo M.A., Sausset A., Gnahoui‐David A., Silva A.R., Brionne A., Le Vern Y., Bussière F.I., Tottey J., Lacroix‐Lamandé S., Laurent F, & Silvestre A. (2019). Eimeria tenella ROP kinase EtROP1 induces G0/G1 cell cycle arrest and inhibits host cell apoptosis. Cellular Microbiology, 21(7), e13027.

+ Open protocol

+ Expand

Lentiviral Transduction and Cell Sorting

Check if the same lab product or an alternative is used in the 5 most similar protocols

For confocal microscopy and flow analysis, FB were stably transduced with a lentiviral vector expressing GFP (sc-108084, Santa Cruz, Dallas, Texas) designed to self-inactivate after transduction and integration of copGP constructs into the genomic DNA of target cells. The cells to be transduced were seeded into six-well plates at a density of 1000 cells/cm² and cultured overnight. The following day, viral particles were added to the cells at a MOI of 4.0 and incubated at 37°C. A complete media change was done after 24 hours and stably transfected cells were then selected using 6.0 µg/mL puromycin treatment. Highly fluorescent cells were then sorted out using a MoFlo Astrios EQ (Beckman Coulter) instrument, and were subsequently used for further experiments.

Tiruvannamalai Annamalai R., Rioja A.Y., Putnam A.J, & Stegemann J.P. (2016). Vascular Network Formation by Human Microvascular Endothelial Cells in Modular Fibrin Microtissues. ACS biomaterials science & engineering, 2(11), 1914-1925.

+ Open protocol

+ Expand

Isolation and Analysis of Single Cells

Check if the same lab product or an alternative is used in the 5 most similar protocols

The obtained tissues were cut into small pieces and shaken with Liberase TL (100 U/mL; Roche) with DNase I (100 U/mL; Sigma) in a 37 °C water bath for 20 min. Subsequently, the cell suspensions were passed through a 70-μm cell strainer to produce single cells. The collected peripheral blood samples were subjected to red blood cell lysis (BioLegend) and then washed twice with PBS. Single-cell suspensions, tissues, and blood samples were incubated with the appropriate antibodies (Additional file 1: Table S1) for 15 min in the dark. The cells were washed twice, resuspended in PBS, and analyzed by MoFlo Astrios EQ (Beckman Coulter) for at least three independent experiments. The data were analyzed using FlowJo V10.0 (Tree Star). Information on the antibodies is listed in Additional file 1: Table S1.

Liu H., Zhu X., Cao X., Chi A., Dai J., Wang Z., Deng C, & Zhang M. (2021). IL-1β-primed mesenchymal stromal cells exert enhanced therapeutic effects to alleviate Chronic Prostatitis/Chronic Pelvic Pain Syndrome through systemic immunity. Stem Cell Research & Therapy, 12, 514.

+ Open protocol

+ Expand

Multiparametric Flow Cytometry of Liver NPCs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Liver NPCs were washed twice with FACS buffer (PBS, pH 7.4, with 1% BSA and 0.05% Sodium Azide) and incubated with nonspecific IgG to assess background fluorescence. Cells were then incubated with anti‐FcγR II/III antibody (Mouse BD Fc Block; BD Pharmingen) for 15 min on ice, followed by staining with a mixture of fluorescence labeled antibodies including PerCP anti‐CD45 (BD bioscience 557235), Alexa Flour 700 anti‐Ly6G (Biolegend 127622), APC‐cy7 anti‐CD11B (Biolegend 101226), and PE‐cy7 anti‐F4/80 (eBioscience 25–4801‐82) for 30 min on ice. Subsequently, cells were washed and permeabilized for 30 min on ice. For Intracellular markers, PE‐Dazzle594 anti‐TNFα (Biolegend 506345), PE anti‐IL1B antigen (Thermo Fisher Scientific), and APC IL6 antigen (BD Bioscience) were stained for 30 min on ice. Cells were washed before measurement. Flow cytometry was performed using MoFlo Astrios EQ (Beckman Coulter Life Sciences) and data was analyzed using FlowJo software (Tree Star Inc.).

Yang W., Kim D.M., Jiang W., Ai W., Pan Q., Rahman S., Cai J.J., Brashear W.A., Sun Y, & Guo S. (2023). Suppression of FOXO1 attenuates inflamm‐aging and improves liver function during aging. Aging Cell, 22(10), e13968.

+ Open protocol

+ Expand

Annexin V-APC and PI Apoptosis Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols

The original culture medium of HTR-8/SVneo cells was first discarded before 1.5 ml 0.25% trypsin without EDTA was added to each plate. After washing with PBS, 1x10⁵ cells were collected and cultured in the dark with 5 µl Annexin V-APC and 5 µl PI staining solution (Nanjing Keygen Biotech Co., Ltd.) for 15 min at room temperature, followed by detection using a flow cytometer (MoFloAstrios EQ; Beckman Coulter, Inc.). Annexin V-APC (+) PI (-) cells were defined as early apoptotic cells whereas Annexin Ⅴ-APC (+) PI (+) cells were defined as late apoptotic cells. The flow cytometry data were analyzed using FlowJo software 8.7.1 (FlowJo LLC). The apoptotic rate was calculated as (early apoptotic cells + late apoptotic cells)/total cells x100%.

Chen L., Shi Q., Fan B, & Cai Y. (2021). Role of lncRNA BCYRN1 in trophoblast cell physiology and pathogenesis of preeclampsia. Experimental and Therapeutic Medicine, 22(4), 1137.

+ Open protocol

+ Expand

Monocyte Subset Identification and iMac Characterization

Check if the same lab product or an alternative is used in the 5 most similar protocols

Monocyte subsets were analyzed by gating for CD14 (PacificBlue; BD Biosciences) and CD16 (PerCPCy5.5; BD Biosciences) in order to distinguish the three subsets of classical/inflammatory (HLA-DR⁺CD16^-CD14⁺), intermediate (HLA-DR⁺CD16⁺CD14⁺) and nonclassical (HLA-DR⁺CD16⁺⁺CD14^-) monocytes, as described earlier (27 (link)). The gating strategy is depicted in Figure S1F. Sorting of monocyte subsets was performed on a MoFlo Astrios EQ (Beckman Coulter) cell sorter. To characterize iMacs, cells were stained for CD206 (APC, eBioscience), CD45 (PE Beckman-Coulter), CD33 (PerCP5.5, Biolegend), CD14 (FITC, Beckman-Coulter), CD11b (PE BD), or with the corresponding isotype controls and analyzed on an Accuri C6 flow cytometer (BD Bioscience).

Craig-Mueller N., Hammad R., Elling R., Alzubi J., Timm B., Kolter J., Knelangen N., Bednarski C., Gläser B., Ammann S., Ivics Z., Fischer J., Speckmann C., Schwarz K., Lachmann N., Ehl S., Moritz T., Henneke P, & Cathomen T. (2020). Modeling MyD88 Deficiency In Vitro Provides New Insights in Its Function. Frontiers in Immunology, 11, 608802.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!