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Arginine vasopressin

Manufactured by Merck Group
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Arginine vasopressin is a peptide hormone that regulates water and salt balance in the body. It is used in laboratory applications for research and testing purposes.

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Lab products found in correlation

Phenylephrine, by Merck Group (4 mentions) Forskolin, by Merck Group (2 mentions) Eticlopride hydrochloride, by Merck Group (2 mentions) Boldenone undecylenate, by Steraloids (2 mentions) Nandrolone decanoate, by Steraloids (2 mentions) Testosterone cypionate, by Steraloids (2 mentions) Phentolamine, by Merck Group (2 mentions) Conivaptan, by Merck Group (2 mentions) Coelenterazine h, by Nanolight Technology (2 mentions) Isoflurane, by Merck Group (1 mentions) 3 isobutyl 1 methylxanthine, by Merck Group (1 mentions) Trichloroacetic acid, by Merck Group (1 mentions) Pentobarbital sodium, by Merck Group (1 mentions) Tolvaptan, by Merck Group (1 mentions) Rapamycin, by Merck Group (1 mentions) Transwell membrane, by Corning (1 mentions) Berberine chloride, by Merck Group (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) 2 deoxy d glucose 2 dg, by Merck Group (1 mentions) 8 bromoadenosine 3 5 cyclic monophosphate 8 br camp, by Merck Group (1 mentions)

12 protocols using arginine vasopressin

1

Investigating Cyclic Nucleotide Signaling

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8-(4-Chlorophenylthio)-adenosine-3',5'-cyclic monophosphate (8-CPT-cAMP), sodium salt, 8-(4-Chlorophenylthio)-guanosine 3′,5′-cyclic monophosphate (8-CPT-cGMP) sodium salt, benzamil (Bz), cetylpyridinium chloride (CPC), 3-Isobutyl-1-methylxanthine (IBMX), forskolin, arginine vasopressin (AVP), [deamino-Cys1, D-Arg8]-vasopressin (dDAVP), trichloroacetic acid, sodium pentobarbital, and isoflurane were obtained from Sigma-Aldrich.
Qian J., Mummalaneni S., Phan T.H., Heck G.L., DeSimone J.A., West D., Mahavadi S., Hojati D., Murthy K.S., Rhyu M.R., Spielman A.I., Özdener M.H, & Lyall V. (2017). Cyclic-AMP regulates postnatal development of neural and behavioral responses to NaCl in rats. PLoS ONE, 12(2), e0171335.
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2

Ovine CRH and AVP-Induced Cortisol Secretion

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A baseline blood sample was taken at time 0 and immediately afterwards sheep were injected with 0.5 μg/kg body weight ovine CRH and 0.1 μg/kg arginine vasopressin (Sigma-Aldrich, St Louis, USA). Blood samples were collected by jugular venepuncture into EDTA vacutainers at 20, 40 and 60 min and centrifuged at 3000 rpm immediately after collection. Samples were stored at -80°C until analysis for plasma cortisol and ACTH concentrations.
Verbeek E., Colditz I., Blache D, & Lee C. (2019). Chronic stress influences attentional and judgement bias and the activity of the HPA axis in sheep. PLoS ONE, 14(1), e0211363.
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3

Microtubule-based Tissue Engineering Protocol

Cited 2 times
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At 2 days, microtubules were harvested from PDMS scaffolds, and transferred onto polyester Transwell membranes (0.4 μm pore size; Cat#CC3460, Corning-Costar, MA, USA) containing 200 μl of 2.4 mg/ml rat tail collagen type I. Following collagen polymerization (45 min at 37 °C), 1.5 ml of culture medium, composed of MEM supplemented with 10% FBS (Invitrogen), 1% l-glutamine and 1% Pen Strep, was added. Samples were cultured for 7 days in the presence of the following agents at doses that were selected on the basis of previous studies: 10 μM forskolin [30 (link)] (Cat#F6886, Sigma-Aldrich), then for another 7 days with 102 μM Octreotide acetate (OCTR) [31 (link)] (Cat#0239950, Toronto Research Chemicals, Brisbane Rd., North York, Canada), 102 μM Pasireotide diaspartate (PAS) [31 (link)] (kind gift from Novartis Farma S.p.A., Varese, Italy), 104 μM 2-Deoxy-d-glucose (2DG) [32 ] (Cat#D6134, Sigma-Aldrich), 10 μg/ml berberine chloride [33 (link)] (Cat#B3251, Sigma-Aldrich), 0.5 μM Rapamycin [34 (link)] (Cat#R0395, Sigma-Aldrich), or 10−2 μM Arginine Vasopressin (AVP) (Cat#V9879, Sigma-Aldrich) alone or in combination with 10−2 μM Tolvaptan [35 (link)] (Cat#T7455, Sigma-Aldrich). For OCTR and PAS dosing, we based this on data from Masyuk et al. [31 (link)] to optimize the best dosage for our system. Control samples were maintained in culture medium alone for 14 days.
Benedetti V., Brizi V., Guida P., Tomasoni S., Ciampi O., Angeli E., Valbusa U., Benigni A., Remuzzi G, & Xinaris C. (2018). Engineered Kidney Tubules for Modeling Patient-Specific Diseases and Drug Discovery. EBioMedicine, 33, 253-268.
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4

Cellular Stimulation Assays

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Following several washes in PBS, cells were incubated in serum-free DMEM-BSA and stimulated with 50nM CRH (Sigma-Aldrich, USA), 100nM arginine vasopressin (Sigma-Aldrich), 5mM 8-bromoadenosine 3′,5′-cyclic monophosphate (8-Br-cAMP; Sigma-Aldrich), and/or 10nM dexamethasone (DEX; Sigma-Aldrich) for the time points indicated. Media was then diluted and assessed via enzyme linked immunosorbant assay (MD Bioproducts, USA) according to manufacturer instructions.
Lu J., Montgomery B.K., Chatain G.P., Bugarini A., Zhang Q., Wang X., Edwards N.A., Ray-Chaudhury A., Merrill M.J., Lonser R.R, & Chittiboina P. (2017). Corticotropin Releasing Hormone can Selectively Stimulate Glucose Uptake in Corticotropinoma via Glucose Transporter 1. Molecular and cellular endocrinology, 470, 105-114.
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5

Neuroendocrine Compound Preparation

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Testosterone cypionate, nandrolone decanoate, and boldenone undecylenate were purchased from Steraloids Inc (Newport, RI) and prepared in sesame oil. Eticlopride hydrochloride (ETIC), serotonin hydrochloride (5HT) and arginine vasopressin (AVP) were purchased from Sigma Aldrich (St. Louis, MO). The ETIC and AVP were dissolved in 0.9% (wt/vol) normal saline, and the 5HT was dissolved in ddH2O.
Morrison T.R., Sikes R.W, & Melloni RH J.r. (2015). ANABOLIC STEROIDS ALTER THE PHYSIOLOGICAL ACTIVITY OF AGGRESSION CIRCUITS IN THE LATERAL ANTERIOR HYPOTHALAMUS. Neuroscience, 315, 1-17.
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6

Anabolic Steroids Administration Protocol

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Testosterone cypionate, nandrolone decanoate, and boldenone undecylenate were purchased from Steraloids Inc (Newport, RI) and prepared in sesame oil (SO). Eticlopride hydrochloride (ETIC) and arginine vasopressin (AVP) were purchased from Sigma Aldrich (St. Louis, MO). The ETIC was dissolved in 0.9% (wt/vol) normal saline and the AVP was dissolved in ddH2O.
Morrison T.R., Ricci L.A, & Melloni RH J.r. (2015). Dopamine D2 Receptors Act Upstream of AVP in the Latero-Anterior Hypothalamus to Modulate Adolescent Anabolic/Androgenic Steroid-Induced Aggression in Syrian Hamsters. Behavioral neuroscience, 129(2), 197-204.
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7

Biochemical Reagents for Cellular Assays

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Ethanol, acetaldehyde, phenylephrine, arginine vasopressin, endothelin-1 and KCl were purchased from SigmaAldrich (St. Louis, MO). PhosphatidylEthanol was purchased from Avanti Polar Lipids, Inc (Alabaster, AL).
Eby J.M, & Majetschak M. (2019). Effects of ethanol and ethanol metabolites on intrinsic function of mesenteric resistance arteries. PLoS ONE, 14(3), e0214336.
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8

Receptor Interaction Profiling in Cells

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The antibodies anti-α1B-AR (host: rabbit; catalog#: ab169523), anti-α1D-AR (host: rabbit; catalog#: ab84402), and anti-CXCR4 (host: goat, ab1670) were obtained from Abcam; anti-AVPR1A (host: rabbit; catalog#: bs-11598R) was from Bioss; anti-AVPR1A (host: mouse, catalog#: LS-C126889) and anti-CCR8 (host: goat; catalog#: LS-C187704) were from LifeSpan Biosciences; anti-CXCR1 (host: rabbit, catalog#: PA5-33452) was from Invitrogen; and anti-CCR1 (host: mouse; catalog#: MAB145), anti-CCR2 (host: mouse; catalog#: MAB48607), IgG isotype control (host: rabbit, catalog#: MAB1050), IgG isotype control (host: mouse, catalog#: MAB004), and IgG isotype control (host: goat, catalog#: AB-108-C) were from R&D Systems. CCL2, CCL2325-99, CXCL8, and CXCL12 were purchased from Protein Foundry. Phenylephrine, phentolamine, arginine vasopressin, conivaptan, and poly-L-lysine were purchased from Sigma-Aldrich. AVPR1A siRNA and nontargeting (NT) siRNA and Accell transfection media were purchased from GE Dharmacon. Proximity ligation rabbit, mouse, and goat +/− probes and detection reagents were purchased from Sigma-Aldrich.
Enten G.A., Gao X., McGee M.Y., Weche M, & Majetschak M. (2024). Chemokine receptor hetero-oligomers regulate monocyte chemotaxis. Life Science Alliance, 7(8), e202402657.
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9

Detailed Pharmacological Reagents Protocol

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AngII, poly-L-ornithine, arginine–vasopressin, Bradykinin and Isoproterenol were from Sigma. Prostaglandin F2α was from Cayman Chemical (Ann Arbor, MI). [Sar1, Ile8]-AngII (SI) and [Asp1, Val5, Gly8]-AngII (DVG) were synthesized at the Université de Sherbrooke (Canada, QC). SR121463B was kindly provided by Sanofi Aventis (Bridgewater, NJ). DCPMP was synthetized at the medicinal chemistry platform of the Institute for Research in Immunology and Cancer (IRIC, Montreal, Canada). SLIGKV-NH2 and salbutamol were from Tocris Bioscience (Bristol, United Kingdom). Iodine-125 was obtained from PerkinElmer. Dulbecco's modified Eagles medium (DMEM), fetal bovine serum (FBS) and other cell culture reagents were purchased from Invitrogen. Prolume Purple (Methoxy e-CTZ), coelenterazine 400a and coelenterazine-h were purchased from Nanolight Technology and Goldbio. Phusion DNA polymerase was from Thermo Scientific. Restriction enzymes and T4 DNA ligase were obtained from NEB. HTS was performed at the IRIC (Université de Montreal) using 1,260 compounds from Microsource Discovery Spectrum library (msdiscovery.com).
Namkung Y., Le Gouill C., Lukashova V., Kobayashi H., Hogue M., Khoury E., Song M., Bouvier M, & Laporte S.A. (2016). Monitoring G protein-coupled receptor and β-arrestin trafficking in live cells using enhanced bystander BRET. Nature Communications, 7, 12178.
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10

Vasoconstrictors and Chemokine Signaling

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Phenylephrine (PE), phentolamine, arginine vasopressin (aVP), conivaptan and AMD3100 were purchased from Sigma-Aldrich. CXCL12 was purchased from Protein Foundry. Coelenterazine H was from Nanolight Technology.
Gao X., Enten G.A., DeSantis A.J, & Majetschak M. (2021). Class A G protein-coupled receptors assemble into functional higher-order hetero-oligomers. FEBS letters, 595(14), 1863-1875.
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