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Snail

Manufactured by R&D Systems
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The Snail is a lab equipment product from R&D Systems. It is designed for the purpose of sample handling and processing in a laboratory setting.

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Lab products found in correlation

P gsk3β ser9, by Cell Signaling Technology (1 mentions) N cadherin, by Proteintech (1 mentions) Polyvinylidene difluoride membrane, by Merck Group (1 mentions) β catenin, by Cell Signaling Technology (1 mentions) E cadherin, by Cell Signaling Technology (1 mentions) C myc, by Proteintech (1 mentions) Bca protein assay kit, by Beyotime (1 mentions) Gapdh, by Proteintech (1 mentions) Cyclin d1, by Proteintech (1 mentions) Gsk3β, by Proteintech (1 mentions) Pecam 1, by BD (1 mentions) Sox17, by R&D Systems (1 mentions) Gata6, by Cell Signaling Technology (1 mentions) Cd105, by R&D Systems (1 mentions) Laminin, by Merck Group (1 mentions) N cadherin, by BD (1 mentions) β catenin, by BD (1 mentions) Vimentin, by R&D Systems (1 mentions) E cadherin, by R&D Systems (1 mentions) Survivin, by Cell Signaling Technology (1 mentions)

Spelling variants of this product

Anti-Snail (5 citations) Snail (AF3639). (2 citations)

5 protocols using snail

1

Protein Expression Analysis in Cell Lines

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The total protein of cells at the logarithmic phase was extracted by a lysis buffer. BCA Protein Assay Kit (Beyotime, Shanghai, China) was used to measure the total protein concentration. The harvested samples were heated for 10 min at 100 °C for denaturation. About 30 μg proteins were separated by gel electrophoresis and transferred to a polyvinylidene difluoride membrane (0.45 μm, Millipore, Billerica, MA, USA). The PVDF membrane was blocked with skimmed milk and then incubated with primary antibodies overnight at 4 °C. After incubation with HRP goat anti-rabbit or goat anti-mouse IgG antibody (Proteintech, Wuhan, China), the protein signal was captured with autoradiography films. The E-Cadherin, p-GSK-3βSer9 and β-catenin antibodies were all obtained from Cell Signaling Technology. GSK3β, N-Cadherin, c-Myc, CyclinD1 and Gapdh antibodies were purchased from Proteintech, while EphA5 was purchased from Invitrogen. Snail was purchased from R&D Systems.
Zhang R., Liu J., Zhang W., Hua L., Qian L.T, & Zhou S.B. (2020). EphA5 knockdown enhances the invasion and migration ability of esophageal squamous cell carcinoma via epithelial-mesenchymal transition through activating Wnt/β-catenin pathway. Cancer Cell International, 20, 20.
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2

Immunohistochemistry Antibody Panel for Cell and Tissue Analysis

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The following primary antibodies were used in this study: Beta-catenin (RRID:AB_397555, BD Transduction labs, Billerica, MA, 610154, 1:500), Brachyury (RRID:AB_2200235, R and D, AF2085, 1:100), CD31 (RRID:AB_394819, BD Biosciences, 553373, 1:100) CD105 (RRID:AB_354735, R and D Systems, AF1320, 1:100), E-cadherin (RRID:AB_477600, Millipore Sigma, U3254, 1:200), Endoglin (CD105) (RRID:AB_354735, R and D Systems, AF1320, 1:300), Flk-1 (RRID:AB_355500, R and D Systems, AF644, 1:200) Gata6 (RRID:AB_10705521, D61E4 XP, Cell Signaling, 5851, 1:500), GFP (RRID:AB_300798, Abcam, ab13970), Laminin (RRID:AB_477163, Millipore Sigma, L9393, 1:500), N-cadherin (RRID:AB_2077527, BD Biosciences, 610920, 1:200), Pecam-1 (RRID:AB_394819, BD Biosciences, 553373, 1:200), RFP (Rockland, Limerick, PA, 600-400-379, 1:300), Snail (RRID:AB_2191738, R and D Systems, AF3639, 1:50), Sox2 (RRID:AB_11219471, Thermo Fisher Scientific, 14-9811-82, 1:200), Sox17 (RRID:AB_355060, R and D Systems, AF1924, 1:100), ZO-1 (RRID:AB_87181, Invitrogen, 33–9100, 1:200).
Morgani S.M., Su J., Nichols J., Massagué J, & Hadjantonakis A.K. (2021). The transcription factor Rreb1 regulates epithelial architecture, invasiveness, and vasculogenesis in early mouse embryos. eLife, 10, e64811.
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3

Apoptosis and Cell Proliferation Assay

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Fetal bovine serum (FBS), antibiotics (penicillin-streptomycin), and Dulbecco’s modified Eagle’s medium (DMEM) were obtained from Gibco-BRL (Grand Island, NY, USA); 3-(4, 5-dimethylthiazol-2-yl)-2; dimethyl sulfoxide (DMSO) and in situ Cell Death Detection Kit (TMR red) from Sigma-Aldrich (St. Louis, MO, USA); 5-diphenyltetrazolium bromide (MTT) from Molecular Probes (Eugene, OR, USA). The following antibodies were used: GAPDH, Bcl-2, survivin, caspase-3, caspase-8, cleaved caspase-3, PCNA and p53 (Cell Signalling, Danvers, MA, USA), PARP, and Bcl-xL (Santa Cruz Biotechnology, Dallas, TX, USA), matrix metalloproteinase (MMP)-2 (Bioss Antibodies Inc., Woburn, Massachusetts, USA), MMP-9, Ki-67, and alexa 488/594 (Abcam, Cambridge, UK), and p21, cyclin D1, CDK4, Bak, tBID, E-cadherin, vimentin, and snail (R&D Systems, Minneapolis, MN, USA). Annexin V-FITC Apoptosis Detection Kit, propidium iodide (PI), Hoechst 33342 staining kit, Matrigel, and various caspase inhibitors were purchased from BD Biosciences (San Jose, CA, USA).
Oh J.M., Lee J., Im W.T, & Chun S. (2019). Ginsenoside Rk1 Induces Apoptosis in Neuroblastoma Cells Through Loss of Mitochondrial Membrane Potential and Activation of Caspases. International Journal of Molecular Sciences, 20(5), 1213.
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4

Immunostaining of Pluripotency and EMT Markers

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Gels were rinsed twice with PBS and fixed with 4% paraformaldehyde (Thermo Fisher Scientific) in PBS for 25 min at room temperature. Following fixation, gels were permeabilized with 0.2% Triton X-100 in PBS for 1 h at room temperature. They were then blocked with 3% BSA in PBS containing 0.01% Triton X-100 for 3 h at room temperature. The samples were then incubated for 24 h at 4 °C with the primary antibody Nanog (1:100; α-Rabbit; Cell Signaling Technologies), SOX2 (1:100; α-Rabbit; Cell Signaling Technologies), Oct4 (1:200; α-Rabbit; Cell Signaling Technologies), Vimentin (1:200; α-mouse; Santa Cruz), SNAIL (1:200; α-Goat; R&D Systems), Brachyury (1:100; α-Goat; R&D Systems), or RUNX2 (1:100; Mouse; Cell Signaling Technologies). After washing for 3-5 h at room temperature, samples were incubated overnight at 4 °C with secondary antibody Alexa 647 goat-α-Rabbit (1:1000 in PBS; Invitrogen), Alexa Fluor donkey-α-Goat (1:1000 in PBS; Invitrogen), or Alexa 488 goat-α-mouse (1:1000 in PBS; Invitrogen), DAPI (1:2,000, Invitrogen) and phalloidin-Alexa 555 (1:500; Abcam). After at least 3 hours of washing, samples were mounted in Vectashield (Vector Laboratories) before imaging.
Nguyen R.Y., Cabral A.T., Rossello-Martinez A., Zulli A., Gong X., Zhang Q., Yan J, & Mak M. (2023). Tunable Mesoscopic Collagen Island Architectures Modulate Stem Cell Behavior. Advanced materials (Deerfield Beach, Fla.), 35(16), e2207882.
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5

SDS-PAGE and Western Blotting Assay

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SDS-PAGE and Western blotting were performed according to standard protocols. Cells were lysed in RIPA lysis buffer (50 mM Tris/HCl, pH 8.0, 250 mM NaCl, 1% NP40, 0.5% (w/v) Sodium Deoxycholate, 0.1% Sodium Dodecylsulfate, complete mini protease inhibitor tablets (Roche)). Lysates were sonicated and centrifuged at 16.060 g for 15 min, 4°C. 100 μg of whole cell lysate per lane was separated using 10% SDS-Acrylamide gels and transferred on Immobilon PVDF membranes (Millipore). ECL signals were recorded using a CF440 Imager (Kodak). Antibodies used: CST5 (Santa Cruz, sc-46890, 1:200), p21 (Neomarkers, CP-74, 1:1000), SNAIL (R&D Systems, AF3639, 1:250), β-actin (Sigma, A2066, 1:1000).
Hünten S, & Hermeking H. (2015). p53 directly activates cystatin D/CST5 to mediate mesenchymal-epithelial transition: a possible link to tumor suppression by vitamin D3. Oncotarget, 6(18), 15842-15856.
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