Ab183979

All tissue was fixed, dehydrated, embedded in paraffin, sectioned at 3 μm, and stained with hematoxylin and eosin according to standard protocols. For immunohistochemistry staining, primary antibody incubations with anti-S100 (1:1000, ab183979, Abcam, USA), anti-YAP (1:100, ab52771, Abcam, USA), anti-SOX10 (1:100, ab227680, Abcam, USA), anti-Ki67 (1:1000, 27309-1-AP, Proteintech, USA), anti-SMA (1:100, 55135-1-AP, Proteintech, USA) antibodies, anti-ERK (1:100, 4695, CST, USA) and anti-pERK (1:100, ab201015, Abcam, USA) were performed. The sections were incubated with horseradish peroxidase-labeled secondary antibodies (GK500705, Gene Tech, China). The antibody signals were visualized using diaminobenzidine, and the sections were counterstained with hematoxylin. Microscopic examination of YAP immunohistochemical staining was conducted on the tissue samples by two blinded pathologists. The average intensity of YAP immunoreactivity was scored via 4 random sites located in the S100 positive area.

Hematoxylin–eosin (HE) and immunohistochemical staining were performed by standard techniques as previously described. The following primary antibodies were used: anti-CD34 (ab81289; Abcam), anti-NSE (PA5-27,452; Invitrogen), anti-D2-40 (MA1-83,884; Invitrogen), anti-CA9 (ab184006; Abcam), anti-PCK (ab28455; Abcam), anti-Oligo2 (ab109186; Abcam), anti-SOX9 (#82,630; Cell signaling technology), anti-Inhibin (PA5-81,202; Invitrogen), anti-GFAP (ab7260; Abcam), anti-S100 (ab183979; Abcam), and anti-Ki67 (ab15580; Abcam).