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γ tubulin

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γ-tubulin is a protein that is a component of the microtubule organizing center (MTOC) in eukaryotic cells. It is involved in the nucleation of microtubules, which are critical for various cellular processes such as cell division and intracellular transport.

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Lab products found in correlation

Enhanced chemiluminescence, by Merck Group (2 mentions) Jarid2, by Cell Signaling Technology (2 mentions) H3k27me3, by Abcam (2 mentions) H3k9 14ac, by Cell Signaling Technology (2 mentions) H3k9me3, by Abcam (2 mentions) Dithiothreitol (dtt), by Merck Group (2 mentions) Phenyl methyl sulfonyl fluoride (pmsf), by Merck Group (2 mentions) Protease inhibitor cocktail, by Roche (2 mentions) P stat3, by Abcam (2 mentions) Hif 1α, by BD (2 mentions)

2 protocols using γ tubulin

1

Quantitative Protein Expression Analysis

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Cellular extracts were prepared using a lysis buffer containing 20 mM Tris HCl (pH 7.5), 150 mM NaCl, 15% glycerol, 1% Triton, 25mM β-glycerolphosphate, 50mM NaF, 10mM NaPyrophosphate, orthovanadate, PMSF (all chemicals are from Sigma-Aldrich, St. Louis, MO), Protease inhibitor cocktail (Roche) and 2% SDS. 25 U of Benzonase® Nuclease (EMD Chemicals, Gibbstown, NJ) and 20mM of DTT (Sigma) were added to the lysis buffer right before use. 15 µg of protein (determied by Bradford) was loaded, separated by 4–20% SDS-PAGE, and transferred to polyvinylidene difluoride membranes, blocked with 5% nonfat dry milk for 60 minutes at rt, and incubated overnight at 4°C with primary antibody. After incubation for one hour with horseradish peroxidase-conjugated secondary antibodies, they were visualized by enhanced chemiluminescence (Millipore Corp, Billerica, MA). Antibodies used in this study are: H3K27me3 (1/1000, Abcam, ab6002), H3K9me3 (1/1000, Abcam, ab8898), H3K9/14Ac (1/1000, Cell Signaling, 9677s), EED (1/1000, gift from Dr. Bomsztyk66 (link)), HIF1α (1/2000, BD Biosciences, 610958), LDHA (1/1000, Cell Signaling, 3582), JARID2 (1/1000, Cell Signaling, 13594), pSTAT3 (1/1000, Abcam, Ab76315) and γ-tubulin (1/10000, Promega, G712A).
Sperber H., Mathieu J., Wang Y., Ferreccio A., Hesson J., Xu Z., Fischer K.A., Devi A., Detraux D., Gu H., Battle S.L., Showalter M., Valensisi C., Bielas J.H., Ericson N.G., Margaretha L., Robitaille A.M., Margineantu D., Fiehn O., Hockenbery D., Blau C.A., Raftery D., Margolin A., Hawkins R.D., Moon R.T., Ware C.B, & Ruohola-Baker H. (2015). The metabolome regulates the epigenetic landscape during naïve to primed human embryonic stem cell transition. Nature cell biology, 17(12), 1523-1535.
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2

Quantitative Protein Expression Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols
Cellular extracts were prepared using a lysis buffer containing 20 mM Tris HCl (pH 7.5), 150 mM NaCl, 15% glycerol, 1% Triton, 25mM β-glycerolphosphate, 50mM NaF, 10mM NaPyrophosphate, orthovanadate, PMSF (all chemicals are from Sigma-Aldrich, St. Louis, MO), Protease inhibitor cocktail (Roche) and 2% SDS. 25 U of Benzonase® Nuclease (EMD Chemicals, Gibbstown, NJ) and 20mM of DTT (Sigma) were added to the lysis buffer right before use. 15 µg of protein (determied by Bradford) was loaded, separated by 4–20% SDS-PAGE, and transferred to polyvinylidene difluoride membranes, blocked with 5% nonfat dry milk for 60 minutes at rt, and incubated overnight at 4°C with primary antibody. After incubation for one hour with horseradish peroxidase-conjugated secondary antibodies, they were visualized by enhanced chemiluminescence (Millipore Corp, Billerica, MA). Antibodies used in this study are: H3K27me3 (1/1000, Abcam, ab6002), H3K9me3 (1/1000, Abcam, ab8898), H3K9/14Ac (1/1000, Cell Signaling, 9677s), EED (1/1000, gift from Dr. Bomsztyk66 (link)), HIF1α (1/2000, BD Biosciences, 610958), LDHA (1/1000, Cell Signaling, 3582), JARID2 (1/1000, Cell Signaling, 13594), pSTAT3 (1/1000, Abcam, Ab76315) and γ-tubulin (1/10000, Promega, G712A).
Sperber H., Mathieu J., Wang Y., Ferreccio A., Hesson J., Xu Z., Fischer K.A., Devi A., Detraux D., Gu H., Battle S.L., Showalter M., Valensisi C., Bielas J.H., Ericson N.G., Margaretha L., Robitaille A.M., Margineantu D., Fiehn O., Hockenbery D., Blau C.A., Raftery D., Margolin A., Hawkins R.D., Moon R.T., Ware C.B, & Ruohola-Baker H. (2015). The metabolome regulates the epigenetic landscape during naïve to primed human embryonic stem cell transition. Nature cell biology, 17(12), 1523-1535.
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