Suberanilohydroxamic acid

Aphidicolin, doxorubicin, rapamycin, chloroquine, trichostatin A, suberanilohydroxamic acid, etoposide, tunicamycin, MG132, nocodazole, and roscovitine were obtained from Sigma-Aldrich (St. Louis, MO). Thapsigargin (R&D Systems, Minneapolis, MN) was generously provided by Dr. Kelly Jordan-Sciutto. Aggregatibacter actinomycetemcomitans cytolethal distending toxin B subunit was obtained from Dr. Bruce Shenker. Antibodies recognizing dyskerin and GAPDH were purchased from Santa Cruz Biotechnology (Santa Cruz, CA). GAR1 and NHP2 antibodies were obtained from Proteintech (Chicago, IL). NOP10, fibrillarin, and LC3B antibodies were from Abcam (Cambridge, MA). All other antibodies, including secondary antibodies, were obtained from Cell Signaling Technology (Danvers, MA).

HeLa DR-13-9 cells utilized for homology directed repair have been previously described [16 (link)] and cultured using standard HeLa culturing protocols. UWB1.289 ovarian carcinoma cells were purchased from ATCC (Manassas, VA) and cultured according to manufacturer specifications. HDAC inhibitors trichostatin A (TSA) and suberanilohydroxamic acid (SAHA) were purchased from Sigma-Aldrich (St. Louis, MO). HDAC10 and control siRNAs were synthesized and purchased from Integrated DNA Technologies (Coralville, IA). Sequences for the siRNAs are listed in Table 1. MTT reagent, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and comet assay lysis buffer were purchased from Trevigen (Gaithersburg, MD). SYBR Green used in the comet assay was purchased from Bio-Rad (Hercules, CA).