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Alexa fluor 488 hamster anti mouse cd3e

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Alexa Fluor 488 hamster anti-mouse CD3e is a fluorescently labeled antibody that binds to the CD3e antigen expressed on the surface of mouse T cells. It can be used for the identification and quantification of mouse T cells in flow cytometry applications.

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Lab products found in correlation

Fixable viability stain 510, by BD (2 mentions) Pe cy7 rat anti mouse cd4, by BD (2 mentions) Apc h7 rat anti mouse cd8a, by BD (2 mentions) Apc hamster anti mouse pd 1, by BD (2 mentions) Pe hamster anti mouse ctla, by BD (2 mentions) Facsdiva software, by BD (2 mentions) Facscanto 2, by BD (1 mentions) Lysis buffer, by Merck Group (1 mentions) Rat anti mouse cd16 cd32, by BD (1 mentions) Pe rat anti mouse cd45r b220, by BD (1 mentions) Pe rat anti mouse cd25, by BD (1 mentions) Fortessa, by BD (1 mentions)

3 protocols using alexa fluor 488 hamster anti mouse cd3e

1

Multicolor Flow Cytometry Analysis of Immune Markers

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PBMCs (1x106 cells in each tube) were stained with Alexa Fluor 488 hamster anti-mouse CD3e (BD Biosciences, clone 145-2C11), Pe-Cy7 rat anti-mouse CD4 (BD Biosciences, clone GK1.5), APC-H7 rat anti-mouse CD8a (BD Biosciences, clone 53–6.7), APC hamster anti-mouse PD-1 (BD Biosciences, clone J43), and PE hamster anti-mouse CTLA (BD Biosciences, clone UC10-4F10-11), Fixable Viability Stain 510 (BD Biosciences, cloneR35-95). Corresponding isotype control for each antibody was prepared for appropriate setting of gates during multicolor flow cytometry analysis. All antibodies were pre-titrated for optimal working concentration. Data were acquired on an 8-color FACSCanto II immunocytometry system (BD Biosciences) with BD FACSDiva software (BD Bioscience). Data were exported from BD FACSDiva and analyzed using Flowjo software version 10 (Tree Star, Oregon, USA).
See J.X., Chandramathi S., Abdulla M.A., Vadivelu J, & Shankar E.M. (2017). Persistent infection due to a small-colony variant of Burkholderia pseudomallei leads to PD-1 upregulation on circulating immune cells and mononuclear infiltration in viscera of experimental BALB/c mice. PLoS Neglected Tropical Diseases, 11(8), e0005702.
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2

Multiparametric Analysis of Immune Checkpoint

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In each tube, 1x10^6 PBMCs were stained with Fixable Viability Stain 510 (BD Biosciences, cloneR35-95), Alexa Fluor 488 hamster anti-mouse CD3e (BD Biosciences, clone 145-2C11), Pe-Cy7 rat anti-mouse CD4 (BD Biosciences, clone GK1.5), APC-H7 rat anti-mouse CD8a (BD Biosciences, clone 53–6.7), APC hamster anti-mouse PD-1 (BD Biosciences, clone J43), and PE hamster anti-mouse CTLA (BD Biosciences, clone UC10-4F10-11). Respective isotype control for each antibody was used to place proper gate for backgrounds. All antibodies were pre-titrated for optimal working concentration. Data were acquired on 8-color FACS Canto II (BD Biosciences) immunocytometry system with FACS Diva software (BD Bioscience). Data were analyzed using Flowjo software version 10 (Tree Star, Oregon, USA).
See J.X., Samudi C., Saeidi A., Menon N., Choh L.C., Vadivelu J, & Shankar E.M. (2016). Experimental Persistent Infection of BALB/c Mice with Small-Colony Variants of Burkholderia pseudomallei Leads to Concurrent Upregulation of PD-1 on T Cells and Skewed Th1 and Th17 Responses. PLoS Neglected Tropical Diseases, 10(3), e0004503.
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3

Multiparameter Flow Cytometry Analysis

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Single-cell suspensions were prepared from spleens and red blood cells lysed for 1 minute with lysis buffer (Sigma Aldrich). Fc receptor binding sites were blocked with rat anti-mouse CD16/CD32 (BD Cat: 553141, 0.5 mg/reaction) and cells labelled with: PE Rat Anti-Mouse CD45R/B220 (BD Pharmingen); Alexa Fluor®700 Rat Anti-Mouse F4/80 (Biolegend®); PE-CF594 Rat Anti-Mouse CD11b (BD Horizon); V450 Rat Anti-Mouse CD8a (BD Horizon™); Alexa Fluor®488 Hamster Anti-Mouse CD3e (BD Pharmingen); PerCP-CyTM5.5 Rat Anti-Mouse CD4 (BD Pharmingen); PE Rat Anti-Mouse CD25 (BD Pharmingen). Data were acquired on a BD Fortessa and analysed using FlowJo.
Le Sommer S., Morrice N., Pesaresi M., Thompson D., Vickers M.A., Murray G.I., Mody N., Neel B.G., Bence K.K., Wilson H.M, & Delibegovic M. (2017). Deficiency in protein tyrosine phosphatase PTP1B shortens lifespan and leads to development of acute leukemia. Cancer research, 78(1), 75-87.
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