Reverse phase lc ms

Manufactured by Shimadzu

The Reverse phase LC-MS is an analytical instrument that combines liquid chromatography (LC) and mass spectrometry (MS) technologies. It is used for the separation, identification, and quantification of chemical compounds in complex mixtures. The LC component separates the sample components, while the MS component provides structural information and precise mass measurements of the separated analytes.

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Lab products found in correlation

Esterase from porcine liver, by Merck Group (1 mentions) Sulfatase from aerobacter aerogenes, by Merck Group (1 mentions)

2 protocols using reverse phase lc ms

Enzyme Kinetics in Phosphate Buffer

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Samples were prepared in phosphate buffer (1x strength) and maintained at a pH of 7.16 to 7.25. The substrate concentration was 25 mM for all samples and enzyme reactions. Temperature was adjusted to 37 °C for 24-hour incubation and for image acquisition. Sulfatase from Aerobacter aerogenes and esterase from porcine liver were purchased from Sigma Aldrich. A total of approximately 1 and 10 units of each enzyme, respectively, were used for the enzymatic reactions. Samples were analyzed after enzyme reactions with reverse phase LC-MS (Shimadzu Corp.)

Fernández-Cuervo G., Sinharay S, & Pagel M.D. (2016). A catalyCEST MRI contrast agent that can simultaneously detect two enzyme activities. Chembiochem : a European journal of chemical biology, 17(5), 383-387.

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Enzymatic Hydrolysis Analysis Using LC-MS

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β-gal and β-gus from Escherichia coli were purchased from Sigma-Aldrich as lyophilized. Samples of the agents were prepared as shown in Table S1. The substrate concentrations were varied between 4–50 mM and 0.050–3.125 mM, depending on the experiment and detection method. The temperature was adjusted to 37.0 ± 0.2 °C for enzyme incubation and for image acquisition in MRI and UV–vis experiments. Samples were analyzed after enzyme reactions with reverse-phase LC–MS (Shimadzu Corporation) to verify enzyme hydrolysis.

Fernández-Cuervo G., Tucker K.A., Malm S.W., Jones K.M, & Pagel M.D. (2016). Diamagnetic Imaging Agents with a Modular Chemical Design for Quantitative Detection of β-Galactosidase and β-Glucuronidase Activities with CatalyCEST MRI. Bioconjugate chemistry, 27(10), 2549-2557.

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