Tlc plates 60 f254

The samples of isoproterenol hydrochloride obtained from different final step batches, reference standard of isoproterenol hydrochloride (purity 99.9%) and the starting material, 1-(3,4-bis(benzyloxy)phenyl)-2-(isopropylamino)ethanone hydrochloride (2) used in the synthesis, were provided by Chemical Research Division, Micro Labs Ltd. (Bangalore, India). The catalyst, Pd-C (50% wet), was acquired from Monarch Catalyst Private Limited (Mumbai, India). HPLC grade acetonitrile and methanol were purchased from Spectrochem Pvt Ltd. (Bangalore, India), and formic acid and trifluoroacetic acid were purchased from Sigma-Aldrich (Bangalore, India). The commercial grade ethyl acetate (EtOAc) and isopropyl alcohol (IPA) were procured from Taiwan Fieldrich Corporation (Taipei, China). Purified water by Millipore Milli-Q Plus Purification System (Bradford, PA, USA) was used during the course of experimental studies. CD₃OD was purchased from Cambridge Isotope Laboratories, Inc. (Andover, MA, USA). Potassium bromide used in Fourier transform infrared spectroscopy (FT-IR) was purchased from Merck KGaA (Darmstadt, Germany). TLC plates (60 F₂₅₄) procured from Merck (Delhi, India) was used to check the progress of reaction.

Melting points were determined
on an electrothermal melting point apparatus and are reported uncorrected.
The completion of the reaction and purity of all compounds were checked
on aluminum-coated TLC plates 60 F₂₅₄ (E. Merck) using
chloroform/methanol (9.5:0.5 v/v) as the mobile phase and visualized
under ultraviolet (UV) light or iodine vapor. Elemental analysis (%
C, H, N) was carried out by a Perkin-Elmer 2400 CHN analyzer. IR spectra
of all compounds were taken on a Perkin-Elmer FT-IR spectrophotometer
in KBr. ¹H NMR and ¹³C NMR spectra were recorded
on Bruker (400 MHz) and (100 MHz) spectrometers, respectively, using
DMSO-d₆ as a solvent and TMS as an internal
standard. Chemical shifts are reported in parts per million (ppm).
A mass spectrum was scanned on a Shimadzu LC–MS 2010 spectrophotometer.
Anhydrous reactions were carried out in oven-dried glassware in a
nitrogen atmosphere. Microwave-assisted synthesis was carried out
using Synthos 3000.^{34 (link)}