The mouse corticotroph pituitary derived cell line AtT-20/D16v-F2 (ATCC® CRL-1795) was cultured and maintained in Dulbecco's Modified Eagle's Medium (DMEM) complemented with 10% FBS, 100 U/ml penicillin/streptomycin, 0.024 M of 2-(4(2-hydroxyethyl)-1-piperazine)-ethane sulfonic acid (HEPES), and maintained at 37°C and 5% CO2, under sterile conditions. CHO-K1 cell line(ATCC® CCL-61) expressing recombinant human GHSR1a (GenBank accession number U60179) or GHSR1b (GenBank accession number U60181) were cultured and maintained in Ham's F12 media (Corning #10-080-CV) supplemented with 10% FBS, 2 mM L-Glutamine and 0.4 mg/mL Geneticin.
Att 20 d16v f2
Manufactured by American Type Culture Collection
Sourced in United States
The AtT-20/D16v-F2 is a laboratory equipment item designed for cell culture applications. It is a subclone of the AtT-20 cell line, which is derived from mouse pituitary tumor cells. The core function of this product is to provide a standardized cell line for research purposes.
Automatically generated - may contain errors
Lab products found in correlation
Ham s f12 media, by Corning (1 mentions)
Foetal bovine serum (fbs), by Cellmax (1 mentions)
Bexarotene, by Selleck Chemicals (1 mentions)
Dexamethasone (dex), by Selleck Chemicals (1 mentions)
Att20, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by American Type Culture Collection (1 mentions)
Dmem high glucose medium, by Thermo Fisher Scientific (1 mentions)
Mcf 7, by American Type Culture Collection (1 mentions)
5 protocols using att 20 d16v f2
1
Culturing Pituitary and CHO Cell Lines
2
Culturing Pituitary Corticotroph Cells
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The mouse anterior pituitary corticotroph adenoma cell line AtT‐20/D16v‐F2 was purchased from ATCC, while COS‐7 cell line was purchased from Cell Bank of Type Culture Collection of Chinese Academy of Sciences. AtT‐20 cells and COS‐7 cells were cultured in DMEM (ATCC, 30‐2002) media supplemented with 10% foetal bovine serum (Cellmax, Peking, China) and penicillin‐streptomycin. The cells were incubated at 37°C and 5% CO2 atmosphere. Bexarotene, ATRA, and dexamethasone (DEX) were obtained from Selleck and stored at −80°C. Corticotropin‐releasing factor human (CRH) was obtained from MCE and stored at −80°C.
3
Culturing AtT-20 Pituitary Cells
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The mouse corticotroph pituitary derived cell line AtT-20/ D16v-F2 (ATCC ® CRL-1795) was cultured and maintained in Dulbecco's Modified Eagle's Medium complemented with 10% FBS, 100 U/mL penicillin/streptomycin, 0.024 M of 2-(4[2hydroxyethyl]-1-piperazine)-ethane sulfonic acid, and maintained at 37 ° C and 5% CO 2 , under sterile conditions, as previously reported [29] . The passage numbers used for the experiments were ranged between 20 and 25. Additionally, both cell lines were checked for mycoplasma contamination by PCR [31] .
4
Culturing Diverse Cancer Cell Lines
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GH3, MCF-7, AtT-20/D16v-F2 and Hela cells were obtained from ATCC (American Type Culture Collection, Manassas, VA, USA) and cultured as previously described [11, 15, 16] . The human cervix adenocarcinoma cell line, Hela, was cultured in the DMEM high glucose medium (Gibco by Life Technologies) enriched with 10% FBS.
5
Establishing Pituitary Cell Lines for Research
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Rat pituitary somatolactotroph tumor cell line GH3, mouse pituitary corticotroph tumor cell line AtT-20/D16v-F2, and the C2C12 murine myoblasts cell line were obtained from the American Type Culture Collection (Manassas, VA, USA). GH3 cells expressing hGHRH-R (GH3-GHRHR) were provided by Clara V. Alvarez by co-transfection with the pCMV-hGHRH-R vector and a plasmid conferring geneticin resistance (pCMVneo) and selected with medium supplemented with 400 mg/mL G-418, as described previously [32 (link),33 (link)]. The cells are phenotyped twice per year for expression of pituitary hormone, transcription factors, somatostatin, and GHRH receptors. Cells were maintained at 37 °C in a 5% CO2 humidified atmosphere in DMEM with 10% FBS, 2 mm L-glutamine, penicillin (100 U/mL), streptomycin (100 μg/mL), and 250 ng/mL amphotericin B.
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