Edaravone (3-methyl-1-phenyl-2-pyrazolin-5-one) was purchased from Mitsubishi Pharma Corporation (Tokyo, Japan). DEM, D-galactosamine (Gal), LPS, and methylthiazolyldiphenyl-tetrazolium bromide (MTT) were products from Sigma-Aldrich (St Louis, MO). PD98059 and staurosporine were products of Calbiochem (Billerica, MA). All cell culture consumables and reagents were bought from either Corning Incorporated (Corning, NY) or Gibco (Carlsbad, CA). Antibodies against catalase (CAT), SOD1, phosphorylated p38 MAPK at Thr180/Tyr182, total p38 MAPK, phosphorylated ERK at Thr202/Tyr204, total ERK, and β-actin were bought from Cell Signaling (Beverly, MA).
Phosphorylated p38 mapk at thr180 tyr182
Manufactured by Cell Signaling Technology
Sourced in United States
Phosphorylated p38 MAPK at Thr180/Tyr182 is a laboratory reagent that detects the phosphorylation of the p38 mitogen-activated protein kinase (MAPK) at specific threonine and tyrosine residues. This product is intended for research use only.
Automatically generated - may contain errors
Lab products found in correlation
Total erk, by Cell Signaling Technology (1 mentions)
Total p38 mapk, by Cell Signaling Technology (1 mentions)
β actin, by Cell Signaling Technology (1 mentions)
D galactosamine, by Merck Group (1 mentions)
Beclin 1, by Cell Signaling Technology (1 mentions)
3 methyladenine 3 ma, by Merck Group (1 mentions)
Total extracellular signal regulated kinase erk, by Cell Signaling Technology (1 mentions)
2 protocols using phosphorylated p38 mapk at thr180 tyr182
1
Edaravone Attenuates Oxidative Stress
2
Lycium barbarum Leaf Extract Preparation
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The preparation for LBP extracts (from Lycium barbarum, Ning Xia Huizu Autonomous Region, People's Republic of China) was the same as reported previously (8) . All cell culture consumables and reagents were bought from either Corning (Corning, NY, USA) or Gibco (Carlsbad, CA, USA). Antibodies against catalase (CAT) and ED1 (cluster of differentiation 68; CD68) were bought from Abcam (Cambridge, UK). Beclin-1, autophagy protein 5 (Atg5), nucleoporin p62 (p62), light chain 3B (LC3B), inducible nitric oxide synthase (iNOS), total p38 mitogen-activated protein kinase (MAPK), phosphorylated p38 MAPK at Thr180/Tyr182, total extracellular signal-regulated kinase (ERK), and phosphorylated ERK at Tyr204 antibodies were bought from Cell Signaling (Danvers, MA, USA). Autophagic inhibitor 3-methyladenine (3-MA) and agonist rapamycin were purchased from Sigma-Aldrich (St. Louis, MO, USA).
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