A murine colon cancer cell line Colon26 (aka CT26) [23 (link),67 (link)] and a murine calvaria-derived osteoblastic cell line MC3T3-E1 [68 (link)] were obtained from Cell Bank at RIKEN BioResource Research Center (Tsukuba, Japan). A rapidly metastatic subline, LuM1, was generated from Colon26 as described previously [23 (link),35 (link),36 (link),42 (link),43 (link)]. MMP3-knockout LuM1 (MMP3-KO) cells were generated using the genome-editing method as described below. A murine macrophage-like cell line RAW-D (a subline of RAW264.7) was kindly provided by Prof. Toshio Kukita (Kyushu University, Fukuoka, Japan) [69 (link)]. Colon26, LuM1, MMP3-KO LuM1, and MC3T3-E1 were maintained in RPMI1640 with 10% fetal bovine serum (FBS) and penicillin, streptomycin, and amphotericin B. RAW-D cells were cultured in minimum essential medium (MEM; Wako Pure Chemicals, Osaka, Japan) containing 10% FBS, supplemented with penicillin (100 U/mL) and streptomycin (100 mg/mL).
Mc3t3 e1
Manufactured by Fujifilm
Sourced in Japan, United States
The MC3T3-E1 is a cell line derived from mouse calvaria that can differentiate into osteoblasts. It is commonly used in research related to bone and cartilage development, as well as the study of osteoblast function and mineralization.
Automatically generated - may contain errors
Lab products found in correlation
Mc3t3 e1, by RIKEN BioResource Center (2 mentions)
α mem, by Fujifilm (2 mentions)
Minimum essential medium (mem), by Fujifilm (1 mentions)
Penicillin streptomycin solution 100, by Fujifilm (1 mentions)
Penicillin, by Fujifilm (1 mentions)
Streptomycin, by Fujifilm (1 mentions)
L alanyl l glutamine, by Fujifilm (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Mc3t3e 1 cells subclone 4, by American Type Culture Collection (1 mentions)
Fetal bovine serum (fbs), by Merck Group (1 mentions)
α minimal essential medium αmem, by Fujifilm (1 mentions)
Penicillin streptomycin, by Fujifilm (1 mentions)
L glutamine penicillin streptomycin solution, by Merck Group (1 mentions)
β glycerophosphate, by Fujifilm (1 mentions)
α mem, by Merck Group (1 mentions)
L ascorbic acid, by Fujifilm (1 mentions)
5 protocols using mc3t3 e1
1
Murine Cancer Cell Lines and Macrophages
2
Culturing Murine Osteoblastic Progenitors
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The murine osteoblastic progenitor cell line MC3T3-E1 was obtained from the RIKEN Bioresource Center (RCB1126). MC3T3-E1 cells were maintained in minimum essential Eagle medium, alpha modification (α-MEM; FUJIFILM Wako Pure Chemical CorporationOsaka, Japan), supplemented with 10% fetal bovine serum (FBS; SERANA Brandenburg, Germany), a penicillin–streptomycin solution (×100) (FUJIFILM Wako Pure Chemical Corporation) at 37 °C, and 5% CO2.
3
Culturing Osteoblastic MC3T3-E1 Cells
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MC3T3-E1 cells subclone 4, an osteoblastic cell line derived from mouse calvaria, was obtained from the American Type Culture Collection (ATCC; Manassas, VA, USA). The MC3T3-E1 cells were cultured in α-minimum essential medium (α-MEM; Fujifilm Wako Pure Chemical) supplemented with 10% fetal bovine serum (FBS; Gibco, Grand Island, NY, USA), 1% penicillin (100 U/mL; Fujifilm Wako Pure Chemical), streptomycin (100 μg/mL; Fujifilm Wako Pure Chemical), and l -alanyl-l -glutamine (2 mM; Fujifilm Wako Pure Chemical) in 5% CO2 at 37 °C.
4
Culturing Murine Pre-Osteoblast MC3T3-E1 Cells
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The MC3T3-E1 murine pre-osteoblast cell line was purchased from the RIKEN Cell Bank. MC3T3-E1 cells were grown at 37°C in a moist atmosphere of 5% CO2 in α-minimal essential medium (α-MEM, Wako Pure Chemical Co., Osaka, Japan) supplemented with 10%(v/v) fetal bovine serum (FBS, Sigma-Aldrich, St. Louis, USA) and 1%(v/v) penicillin-streptomycin (Wako Pure Chemical, Osaka, Japan).
5
Osteogenic Differentiation of MC3T3-E1 Cells
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A mouse calvaria-derived preosteoblast cell line, MC3T3-E1, was purchased from RIKEN BioResource Center (Tsukuba, Japan) and used directly. As described in previous reports [28] [29] [30] , cells were cultured at 37°C in a humidified atmosphere of 5% CO2 in air and maintained in a growth medium composed of the minimum essential medium Eagle with alpha modification (α-MEM, Sigma-Aldrich, St. Louis, MO, USA) supplemented with 10% fetal bovine serum and 100 U L-glutamine-penicillinstreptomycin solution (G1146, Sigma-Aldrich).
To induce osteogenic differentiation of the MC3T3-E1 cells, the growth medium was shifted with a differentiation induction medium after 100% confluence [31] [32] [33] , and the differentiation induction medium consisted of growth medium supplemented with 50 mg mL -1 L-ascorbic acid (Wako, Osaka, Japan) and 2 mM β-glycerophosphate (Wako). The differentiation induction medium was replaced every three days.
Before cell seeding, the Zr-14Nb-5Ta-1Mo alloy and Ti specimens were sterilized by immersion in 70% ethanol and then fully rinsed with ultra-pure water.
To induce osteogenic differentiation of the MC3T3-E1 cells, the growth medium was shifted with a differentiation induction medium after 100% confluence [31] [32] [33] , and the differentiation induction medium consisted of growth medium supplemented with 50 mg mL -1 L-ascorbic acid (Wako, Osaka, Japan) and 2 mM β-glycerophosphate (Wako). The differentiation induction medium was replaced every three days.
Before cell seeding, the Zr-14Nb-5Ta-1Mo alloy and Ti specimens were sterilized by immersion in 70% ethanol and then fully rinsed with ultra-pure water.
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