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Glycol chitosan

Manufactured by Fujifilm
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Sourced in Japan

Glycol chitosan is a water-soluble derivative of chitosan. It is a neutral polysaccharide with a molecular weight range of 10,000 to 300,000 Daltons. Glycol chitosan is used as a component in various laboratory applications, such as cell culture, drug delivery, and tissue engineering.

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Lab products found in correlation

Trypsin, by Thermo Fisher Scientific (1 mentions) Pluronic f 127 powder, by Merck Group (1 mentions) Polyethylene glycol peg, by Sinopharm (1 mentions) Rpmi 1640 medium rpmi 1640, by Thermo Fisher Scientific (1 mentions) Penicillin streptomycin p s, by Thermo Fisher Scientific (1 mentions) Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions) Hexanoic anhydride, by Fujifilm (1 mentions) 3 mercaptopropionic acid, by Merck Group (1 mentions) N hydroxysuccinimide nhs, by Merck Group (1 mentions) 1 ethyl 3 3 dimethylaminopropyl carbodiimide hydrochloride edc, by Merck Group (1 mentions) Acetone, by Samchun (1 mentions) Ethanol, by Samchun (1 mentions) Methanol, by Samchun (1 mentions) Nitroblue tetrazolium, by Merck Group (1 mentions) P nitrophenol phosphate, by Merck Group (1 mentions) Recombinant human bone morphogenetic protein 2 rhbmp 2, by R&D Systems (1 mentions) Poly vinylsulfonic acid, by Merck Group (1 mentions) Tween 20, by Merck Group (1 mentions) Alizarin red s, by Merck Group (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions)

6 protocols using glycol chitosan

1

Multifunctional Wound Healing Hydrogel

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Poly (ethylene glycol) (PEG, Mw = 4000, 96%) was supplied by Sinopharm Group Co. Ltd. Glycol chitosan (GC, 82 kDa, 85% degree of deacetylation) was purchased from Wako Pure Chemical Industries, Ltd, Japan. Kangfuxin (KFX) potion was provided by China-Japan Friendship Hospital (Beijing, China). Pluronic® F127 powder (>98%) was purchased from Sigma Aldrich, USA. RPMI 1640 medium (RPMI 1640), penicillin-streptomycin (PS), fetal bovine serum (FBS), and trypsin were provided by Gibco Life Technologies (Beijing, China). The mouse fibroblast cells (L929) were obtained from Cell Resource Center, IBMS, CAMS/PUMC (Beijing, China). Rabbit anti-TNF-α antibody (1 mg mL−1), rabbit anti-IL-6 antibody (1 mg mL−1), goat anti-rabbit IgG/Alexa Fluor 594 antibody, goat anti-mouse IgG/Alexa Fluor 594 antibody and goat anti-rabbit IgG/FITC antibody were purchased from Beijing Boosen Biotechnology Co., Ltd (Beijing, China). RT products and PCR primers were provided by Beijing Dingguochangsheng Biotechnology Co., Ltd (Beijing, China). Anti-EGF antibody (rabbit), anti-VEGFA antibody [VG-1] (mouse) and anti-TGF-β1 antibody (rabbit) were obtained from Abcam Company, USA. All the other reagents (AR grade) were used as received from Sinopharm Chemical Reagent Co., Ltd (Shanghai, China) and used without further purification.
Fu Y.N., Li Y., Deng B., Yu Y., Liu F., Wang L., Chen G., Tao L., Wei Y, & Wang X. (2021). Spatiotemporally dynamic therapy with shape-adaptive drug-gel for the improvement of tissue regeneration with ordered structure. Bioactive Materials, 8, 165-176.
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2

Glycol Chitosan-Based Mucin Conjugation

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Glycol chitosan (GC, DP ≥ 200) and hexanoic anhydride (97%) were purchased from WAKO (Japan) and Sigma-Aldrich (India), respectively. 3-Mercaptopropionic acid, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), and mucin from porcine were purchased from Sigma-Aldrich (St. Louis, MO, USA). Acetone, methanol and ethanol were supplied from Samchun Chemical (Korea).
Cho I.S., Oh H.M., Cho M.O., Jang B.S., Cho J.K., Park K.H., Kang S.W, & Huh K.M. (2018). Synthesis and characterization of thiolated hexanoyl glycol chitosan as a mucoadhesive thermogelling polymer. Biomaterials Research, 22, 30.
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3

Biofunctionalized Polymer Scaffold Synthesis

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Glycol chitosan (GC, ~ 100 kDa) was supplied from Wako Chemical USA, Inc. (Richmond, VA). Glycidyl methacrylate, poly-vinylsulfonic acid (PVSA, ~ 2 kDa), poly-4-styrenesulfonic acid (PSS, ~ 75 kDa), heparin sodium salt from porcine intestinal mucosa (heparin, ~ 18 kDa), Tween-20, p-nitrophenol phosphate, β-glycerophosphate, dexamethasone, nitro blue tetrazolium (NBT) and alizarin red S were purchased from Sigma-Aldrich (St. Louis, MO). Recombinant human bone morphogenetic protein-2 (rhBMP-2) was purchased from R&D Systems (Minneapolis, MN). Mouse bone marrow stromal cells (BMSCs, D1 ORL UVA, CRL-12424) and mouse myoblast (C2C12, CRL-1772) cells were obtained from American Type Culture Collection (ATCC, Manassas, VA).
Kim S., Cui Z.K., Kim P.J., Jung L.Y, & Lee M. (2018). Design of hydrogels to stabilize and enhance bone morphogenetic protein activity by heparin mimetics. Acta biomaterialia, 72, 45-54.
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4

Synthesis and Characterization of Multimodal Polymeric Probes

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Poly(ethylene glycol) (600)-polybutadiene (1200) copolymer (denoted PEG-PBD) was purchased from Polymer Source (Dorval, Quebec, Canada). Glycol chitosan was purchased from Wako Chemicals (Richmond, VA). DTPA dianhydride, gadolinium (III) chloride, fluorescein isothiocyanate-dextran (FITC-dextran MW 59,000–77,000), tetramethylrhodamine isothiocyanate-Dextran (TRITC-dextran, MW 4400) and poly(ethylene glycol)-block-poly(propylene oxide)-block-poly(ethylene glycol) (PEG-PPO-PEG, MW 4400) were obtained from Sigma-Aldrich. Sulforhodamine B (SRB), tetramethylrhodamine-dextran (TRITC-dextran, 10,000 MW) and tetramethylrhodamine-dextran (TRITC-dextran, 40,000 MW) were obtained from Thermo Fisher Scientific Inc. All other chemicals were used as received. All of the buffer solutions were prepared with deionized water.
Yan L., Higbee E., Tsourkas A, & Cheng Z. (2015). A simple method for the synthesis of porous polymeric vesicles and their application as MR contrast agents. Journal of materials chemistry. B, Materials for biology and medicine, 3(48), 9277-9284.
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5

Synthesis of Glycol Chitosan-Platinum Conjugates

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All solvents and chemicals were purchased from commercial suppliers and were used as received. The following chemicals were used without further purification for the synthesis: K2[PtCl4] (Assay: 46.69% Pt) (Johnson Matthey, Zurich, Switzerland), glycol chitosan (Assay: 78.2%) (Wako, Osaka, Japan), succinic anhydride (≥99%) (Sigma Aldrich, Steinheim, Germany), acetic anhydride (≥97.0%) (Fisher Scientific, Schwerte, Germany), absolute DMF (99.8%, extra dry, water <50 ppm) (Acros Organics, Geel, Belgium), absolute DMSO (99.7%, extra dry, water <50 ppm) (Acros Organics, Geel, Belgium), triethylamine (99%) (Acros Organics, Geel, Belgium).
Trial Kit Spectra/Por® 3 (molecular weight cut off (MWCO) = 3.5 kDa) and Spectra/Por® 7 (MWCO = 1.0 kDa) dialysis tubing was purchased from Carl Roth (Karlsruhe, Germany). All aqueous solutions were performed with Milli-Q water (18.2 MΩ cm, Milli-Q Advantage). Reactions containing platinum complexes were conducted with glass-coated magnetic stirring bars and protected from light.
Lerchbammer-Kreith Y., Sommerfeld N.S., Cseh K., Weng-Jiang X., Odunze U., Schätzlein A.G., Uchegbu I.F., Galanski M.S., Jakupec M.A, & Keppler B.K. (2023). Platinum(IV)-Loaded Degraded Glycol Chitosan as Efficient Platinum(IV) Drug Delivery Platform. Pharmaceutics, 15(4), 1050.
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6

Glycol Chitosan-Based Fluorescent Conjugates

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Glycol chitosan (GC, D DP200, degree of acetylation = 9.13 ± 0.89% as determined by 1 H-NMR), diethylene glycol, albumin from bovine serum (BSA) and acetone were purchased from Wako Pure Chemical Industries, Ltd (Osaka, Japan). Dextran (Dex, Mn=50,000-70,000), sodium periodate, sodium cyanoborohydride and fluorescein isothiocyanate isomer Ⅰ (FITC) were purchased from Nacalai tesque (Kyoto, Japan). Tertbutyl carbazate was purchased from Tokyo Chemical Industry Co., Ltd (Tokyo, Japan).
Cho I.S, & Ooya T. (2018). An injectable and self-healing hydrogel for spatiotemporal protein release via fragmentation after passing through needles. Journal of biomaterials science. Polymer edition, 29(2).
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