Anti-mouse CD140b (catalog 136002, RRID: AB_1953332), APC anti-mouse CD140b antibody (catalog 136008, RRID: AB_2268091), Alexa Fluor 647 anti-human CD31 antibody (catalog 303111, RRID:AB_493077), Alexa Fluor 488 anti-human CD31 antibody (catalog 303109, RRID:AB_493075), APC anti-human CD140b (PDGFRβ) antibody (catalog 323608, RRID:AB_2162787), Brilliant Violet 421 anti-mouse CD45 antibody (catalog 103133, RRID:AB_10001045), BV421 anti-human CD117 (C-kit) antibody (catalog 313215, RRID:AB_10896056), BV421 anti-mouse CD117 (catalog 560557, RRID:AB_1645258), PE/Cy5 anti-human CD45 (catalog 304010, RRID:AB_314398), APC anti-mouse FCεRIα (catalog 134316, RRID:AB_10640121), Brilliant Violet 711 anti-human TNF-α antibody (catalog 502940, RRID AB_2563885), and APC anti-mouse TER-119/erythroid cells antibody (catalog 116212 RRID AB_313713) were from BioLegend. Mouse/rat CD31/PECAM-1 antibody (catalog AF3628, RRID:AB_2161028), mouse mast cell protease-6/Mcpt6 antibody (catalog MAB3736, RRID:AB_2240825), and BV510 anti-human FCεRIα (catalog 334626, RRID:AB_2564291) were from R&D Systems
Apc anti mouse cd140b antibody
Manufactured by BioLegend
The APC anti-mouse CD140b antibody is a flow cytometry reagent used to detect the expression of CD140b (also known as PDGFR-β) on mouse cells. CD140b is a tyrosine kinase receptor that plays a role in cell proliferation, survival, and differentiation. This antibody can be used to identify and characterize cells expressing CD140b.
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Lab products found in correlation
Apc anti mouse fcεriα, by BioLegend (1 mentions)
Alexa fluor 647 anti human cd31 antibody, by BioLegend (1 mentions)
Alexa fluor 488 anti human cd31 antibody, by BioLegend (1 mentions)
Pe cy5 anti human cd45, by BioLegend (1 mentions)
Facsdiva software, by BD (1 mentions)
Facsaria fusion, by BD (1 mentions)
Apc goat anti mouse igg, by BioLegend (1 mentions)
Apc mouse anti human cd90, by BD (1 mentions)
Apc anti human cd31 antibody, by BioLegend (1 mentions)
2 protocols using apc anti mouse cd140b antibody
1
Multicolor Flow Cytometry Analysis of Mouse and Human Cells
2
Cardiomyocyte FACS Sorting and Characterization
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Differentiated EBs were dissociated the same way as in the high throughput screening and suspended in 2% FBS/PBS. We analyzed and sorted the CMs using FACSAria Fusion (BD Biosciences), FACSDiva software (BD Biosciences) and FlowJo software. For Ki-67 staining, we used purified mouse anti-Ki-67 Clone B56 (RUO) (BD Biosciences, 556003, 1:200 dilution) and APC goat anti-mouse IgG (minimal x-reactivity) (Biolegend, 405308, 1:500 dilution). To sort the CMs derived from 1390C1 or 409B2, we used PE/Cyanine7 anti-human CD172a/b (SIRPα/β) antibody (BioLegend, 323808, 1:500 dilution), APC mouse anti-human CD90 (BD, 559869, 1:1000 dilution), APC anti-human CD31 antibody (BioLegend, 303116, 1:500 dilution), Alexa Fluor® 647 anti-human CD49a antibody (BioLegend, 328310, 1:500 dilution) and APC anti-mouse CD140b antibody (BioLegend, 136008, 1:500 dilution).
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