The bacterial strain used in the study, C. violaceum 30191, was obtained from the Leibniz-Institute DSMZ-German Collection of Microorganisms and Cell Cultures (DSMZ, Braunschweig, Germany). It was stored in 8% DMSO at −80 °C. Before each experiment, the strain was inoculated in Luria-Bertani Broth (LB)—1% Casein enzymic hydrolysate, 0.5% yeast extract, 1% NaCl, (HiMedia, Modautal, Germany), and maintained at 4 °C on Luria-Bertani Agar (LA)—1% Casein enzymic hydrolysate, 0.5% yeast extract, 1% NaCl, 0.15% agar (HiMedia, Modautal, Germany) slants. As a source of bacterial inoculum, before each experiment, the strain was incubated in LB, without shaking at 30 °C for 24 h.
Casein enzymic hydrolysate
Manufactured by HiMedia
Sourced in India
Casein enzymic hydrolysate is a readily available source of amino acids and peptides derived from the enzymatic hydrolysis of casein, a milk protein. It serves as a nutritional component in cell culture media and other biological applications.
Automatically generated - may contain errors
Lab products found in correlation
Graphene oxide, by Merck Group (2 mentions)
Oxgall, by HiMedia (2 mentions)
Bile salt, by HiMedia (2 mentions)
Ponddtox, by Novozymes (2 mentions)
Sodium thiosulphate, by HiMedia (2 mentions)
Sodium deoxycholate, by HiMedia (2 mentions)
Sodium lauryl sulphate, by HiMedia (2 mentions)
Potassium tellurite, by HiMedia (2 mentions)
D glucose anhydrous, by HiMedia (2 mentions)
Tcbs agar, by HiMedia (2 mentions)
Nutrient broth, by HiMedia (2 mentions)
Nutrient agar, by HiMedia (2 mentions)
Sucrose, by HiMedia (2 mentions)
Sodium chloride (nacl), by HiMedia (2 mentions)
Luria bertani broth, by HiMedia (2 mentions)
Yeast extract, by HiMedia (2 mentions)
Proteose peptone, by HiMedia (2 mentions)
Sodium citrate, by HiMedia (2 mentions)
3 protocols using casein enzymic hydrolysate
1
Cultivation and Storage of C. violaceum
2
Fabrication of Asymmetric Membranes with Antimicrobial Properties
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Polyvinylidene uoride (MW = 573 KDa, Solef, Solvay, France), polyester fabric (Filtration Sciences Corporation, USA), N, N dimethyl formamide (Qualigen, India), D-(+)-Glucose anhydrous (Hi media, India), Graphene oxide (Sigma-Aldrich) and 2, 3, 5-Triphenyltetrazolium chloride (≥ 99.0% SIGMA, Life Science) were used to prepare the asymmetric membranes. Reverse osmosis treated water was used for the membrane preparation.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
3
Fabrication of Asymmetric Membranes with Antimicrobial Properties
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Polyvinylidene uoride (MW = 573 KDa, Solef, Solvay, France), polyester fabric (Filtration Sciences Corporation, USA), N, N dimethyl formamide (Qualigen, India), D-(+)-Glucose anhydrous (Hi media, India), Graphene oxide (Sigma-Aldrich) and 2, 3, 5-Triphenyltetrazolium chloride (≥ 99.0% SIGMA, Life Science) were used to prepare the asymmetric membranes. Reverse osmosis treated water was used for the membrane preparation.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
Nutrient Agar, Nutrient Broth, Luria-Bertani Broth and TCBS Agar (Hi media, India) were used for all microbiology studies. Escherichia coli (NCIM2065), Bacillus subtilis (NCIM2920), Vibrio cholerae (N16961), Vibrio parahaemolyticus (IDH02640), Vibrio campbellii, Vibrio harveyi, Vibrio proteolyticus (Isolated from seawater and identi ed in our laboratory) were used for testing and evaluating the vibrio kit. Also, commercial probiotic containing Paracoccus pantotrophus (PondDtox, Novozymes) was used to test the vibrio kit.
Casein enzymic hydrolysate, Yeast extract, Proteose peptone, Sucrose, Sodium thiosulphate, Sodium citrate, Sodium deoxycholate, Sodium chloride, Oxgall, Sodium lauryl sulphate, Bile salts and Potassium tellurite (HiMedia, India) were used to prepare Vibrio selective medium.
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