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Absolute hydrochloric acid

Manufactured by Merck Group
Sourced in Germany

Absolute Hydrochloric acid is a concentrated, pure form of hydrochloric acid. It is a colorless, fuming liquid with a pungent odor. Hydrochloric acid is a common laboratory reagent used for various applications in scientific research and analysis.

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2 protocols using absolute hydrochloric acid

1

Transfersome and Liposome Formulation Protocol

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Analytical grade Chloroform of Merck and analytical grade Methanol of Sigma Andrich were used as organic solvents for the formulation of transfersome and liposomes. Absolute Hydrochloric acid (Merck) and Sodium Hydroxide (Sigma-Aldrich, Germany) were used for the quantitative analysis of Glucosamine sulfate as per official compendia method. Triethylamine procured from Merck was used for the formulation of hydrogel. Eugenol was purchased from Sigma Scientific Pvt limited. Phosphate Buffer saline (PBS) solution having PH. 7.0 was made from PBS tablets purchased from Sigma Scientific. Distilled water was taken from pharmaceutics lab, Department of Pharmacy, Riphah International University (Islamabad, Pakistan).
For the formulation of transfersome, Phospholipid 90G was generously gifted by Lipoid Germany, Glucosamine sulfate was gifted from Polyline ChemPharma Peshawar, Polyoxyethylene (20) sorbitan monooleate (Tween 80) and sorbitan monostearate (Span 60) were procured from Merck. For the induction of osteoarthritis in knee joint of rabbits l-Cysteine and Papain were procured from Sigma Adrich Germany. All the chemicals were of pure analytical grade.
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2

Hydrogel Preparation Using Natural Biopolymers

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ALG with low viscosity (viscosity of 1% aq solution: 40-90 mPa s) were obtained by Tate & Lyle Group (Tate & Lyle, London, UK). GT (Rotel Inc., İstanbul, Turkey) and XN (Jungbunzlauer, Basel, Switzerland) are gum types used in hydrogel preparation. CH (deacetylated chitin, Poly(d-glucosamine) with medium molecular weight) was purchased from Sigma-Aldrich (St. Louis, MO) to be used in coating solution. Honey (Binboga Bal, Turkey) and calcium chloride (Merck, USA) were used in different coating formulations. As a model active agent, PC was supplied from Konya Seker Inc. (Konya, Turkey). Chemicals as sodium hydroxide, sodium chloride were obtained from Sigma-Aldrich (St. Louis, MO). Absolute hydrochloric acid (37%, with density of 1.19 g/cm 3 at 20 °C, boiling point of 45 °C, melting point of -28 °C) and potassium dihydrogen phosphate (KH 2 PO 4 ) (EMSURE ISO) were obtained from Merck (Germany).
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