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6 protocols using d glyceraldehyde

1

Catalytic Conversion of Carbohydrates

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d-(+)-Mannose (MAN, 99%), d-(+)-glucose (GLU, 99.5%), d-(−)-fructose (FRU, 99%), glycolaldehyde dimer (GA, 95%) and d-(−)-glyceraldehyde (GLA, 90%) were purchased from Sigma-Aldrich. Methyl glycolate (MG, 98%), 1,3-dihydroxyacetone (DHA, 99%), pyruvaldehyde (PYR, 40%), methyl lactate (ML, 98%), methanol (99.9%) were purchased from Macklin. Methyl glycerate (MGLY, 95%) was purchased from Molbase. Molybdenum(vi) oxide (MoO3) and potassium carbonate were purchased from Sinopharm Chemical Reagent Company. Gold nanoparticle supported on titanium oxides (Au/TiO2: gold 1% on titanium dioxide) was purchased from Aladdin. Deionized water was produced by a laboratory water purification system.
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2

Bovine Albumin and Glyceraldehyde Protocol

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Bovine serum albumin (BSA) (essentially fatty acid free) and D-glyceraldehyde were purchased from Sigma (St. Louis, MO, USA). TNF-α was purchased from Cell Signaling Technology (Danvers, MA, USA).
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3

Monocyte CD36 Regulation Protocol

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BSA and d-glyceraldehyde were purchased from Sigma-Aldrich (St. Louis, MO, USA). An antioxidant, NAC, and anti-CD36 antibody were purchased from Abcam (ab143032 and ab23680, respectively, Cambridge, UK). A selective Cdk5 inhibitor, (R)-DRF053, was obtained from R&D System, Inc. (Minneapolis, MN, USA). A human monocytic cell line, U 937 cells (JCRB9021), was purchased from Japanese Collection of Research Bioresources (JCRB, Osaka, Japan). Dil-ox-LDL was obtained from Highland Technology Center (Frederick, MD, USA). Phorbol 12-myristate 13-acetate was purchased from Sigma Aldrich (St. Louis, MO, USA).
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4

Glyceraldehyde Synthesis and Purification

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D,L-glyceraldehyde-3-phosphate was purchased from Santa Cruz Biotechnology, Inc. D-glyceraldehyde (GA) and other reagents were purchased from Sigma-Aldrich.
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5

Fatty Acid-Free BSA Extraction

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Bovine serum albumin (BSA) (essentially fatty acid free and essentially globulin free, lyophilized powder) and D-glyceraldehyde were purchased from Sigma (St. Louis, MO, USA). Ethyl acetate and n-butanol were purchased from Wako Pure Chemical Industries, Ltd. (Osaka, Japan).
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6

Cell Culture Conditions and Compounds

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Cell lines were maintained in DMEM (Thermo Fisher Scientific Waltham, MA, USA) medium, without glucose, glutamine, phenol red, and sodium pyruvate. The media were supplemented with 10% fetal bovine serum (Thermo Fisher Scientific Waltham, MA, USA), 2 mM glutamine (Thermo Fisher Scientific Waltham, MA, USA), and 2.5 g/L glucose (Merck, Darmstadt, Germany) and cultivated at 37 °C , 5% CO2, 21% O2 and 85% relative humidity. To avoid contact inhibition, cells were passaged every 3–4 days. The neuroblastoma cell lines BE(2)-C (RRID: CVCL_0529) were obtained from ECACC (Salisbury, UK). The IMR-32 (RRID: CV CL_0346), GI-ME-N (RRID: CVCL_1232) and SH-SY5Y (RRID: CVCL_0019) cell lines were purchased from the DSMZ (Braunschweig, Germany). Cell lines were authenticated via the Multiplex human Cell line Authentication Test (Multiplexion, Immenstaad, Germany). The active primary human foreskin fibroblasts from an infant donor (VH7) were a gift from Petra Boukamp (German Cancer Research Center (DKFZ), Heidelberg, Germany). L-glyceraldehyde (73572, Sigma-Aldrich, St. Louis, MO, USA), D-glyceraldehyde (49800, Sigma-Aldrich, St. Louis, MO, USA) were prepared in 1M stock solutions in PBS and stored at 4% before use. N-acetyl-cysteine (A9165, Sigma-Aldrich, St. Louis, MO, USA) was prepared in a 500 mM solution in PBS fresh before use in cell culture.
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