Log In Sign up
The largest database of trusted experimental protocols

Plc5 cir gfp

Manufactured by Geneseed
Visit Supplier
Sourced in China

The PLC5-ciR-GFP is a plasmid construct that contains a constitutively expressed green fluorescent protein (GFP) reporter gene. It is designed for use in basic molecular biology and cell biology research applications.

Automatically generated - may contain errors

Lab products found in correlation

Ribofect cp transfection solution, by RiboBio (4 mentions) Antisense oligonucleotide aso, by RiboBio (3 mentions)

4 protocols using plc5 cir gfp

1

Overexpression and Knockdown of circ-LRIG3

Check if the same lab product or an alternative is used in the 5 most similar protocols
To overexpress circ-LRIG3, pLC5-ciR-GFP (#GS0108) vector containing one paired reverse complementary sequence (RCS) provided by Geneseed (Guangzhou, China) was used, in which the full-length of circ-LRIG3 (chr12: 59277301–59,308,117) was inserted into pLC5-ciR vector between EcoRI and BamHI restriction sites, followed by packaging into the lentivirus and infection into HepG2 cells. The stable circ-LRIG3-overexpressing cells were selected by 0.8 μg/mL puromycin. To knock down circ-LRIG3, two antisense oligonucleotide (ASO) against circ-LRIG3 junction site were designed and synthesized by RiboBio (Guangzhou, China). Then, the ASO was transfected into SMMC-7721 cells using riboFECT™ CP transfection solution (RiboBio) based on manufacturer’s protocol.
Sun S., Gao J., Zhou S., Li Y., Wang Y., Jin L., Li J., Liu B., Zhang B., Han S., Ding H, & Li X. (2020). A novel circular RNA circ-LRIG3 facilitates the malignant progression of hepatocellular carcinoma by modulating the EZH2/STAT3 signaling. Journal of Experimental & Clinical Cancer Research : CR, 39, 252.
+ Open protocol
+ Expand
2

Modulating Circular RNA LRIG3 in Liver Cancer

Check if the same lab product or an alternative is used in the 5 most similar protocols
To overexpress circ-LRIG3, pLC5-ciR-GFP (#GS0108) vector containing one paired reverse complementary sequence (RCS) provided by Geneseed (Guangzhou, China) was used, in which the full-length of circ-LRIG3 (chr12: 59277301-59308117) was inserted into pLC5-ciR vector between EcoRI and BamHI restriction sites, followed by packaging into the lentivirus and infection into HepG2 cells. The stable circ-LRIG3-overexpressing cells were selected by puromycin. To knock down circ-LRIG3, two antisense oligonucleotide (ASO) against circ-LRIG3 junction site were designed and synthesized by RiboBio (Guangzhou, China). Then, the ASO was transfected into SMMC-7721 cells using riboFECT™ CP transfection solution (RiboBio) based on manufacturer's protocol.
Sun S., Gao J., Zhou S., Li Y., Wang Y., Li J., Li J., Liu B., Zhang B., Han S., Ding H., & Li X. (2020). A Novel Circular RNA circ-LRIG3 Facilitates the Malignant Progression of Hepatocellular Carcinoma by Modulating the EZH2/STAT3 Signaling.
+ Open protocol
+ Expand
3

Overexpression and Knockdown of circ-LRIG3

Check if the same lab product or an alternative is used in the 5 most similar protocols
To overexpress circ-LRIG3, pLC5-ciR-GFP (#GS0108) vector containing one paired reverse complementary sequence (RCS) provided by Geneseed (Guangzhou, China) was used, in which the full-length of circ-LRIG3 (chr12: 59277301-59308117) was inserted into pLC5-ciR vector between EcoRI and BamHI restriction sites, followed by packaging into the lentivirus and infection into HepG2 cells. The stable circ-LRIG3-overexpressing cells were selected by 0.8μg/mL puromycin. To knock down circ-LRIG3, two antisense oligonucleotide (ASO) against circ-LRIG3 junction site were designed and synthesized by RiboBio (Guangzhou, China). Then, the ASO was transfected into SMMC-7721 cells using riboFECT™ CP transfection solution (RiboBio) based on manufacturer's protocol.
Sun S., Gao J., Zhou S., Li Y., Wang Y., Li J., Li J., Liu B., Zhang B., Han S., Ding H., & Li X. (2020). A novel circular RNA circ-LRIG3 facilitates the malignant progression of hepatocellular carcinoma by modulating the EZH2/STAT3 signaling.
+ Open protocol
+ Expand
4

Overexpression and Knockdown of circ-LRIG3

Check if the same lab product or an alternative is used in the 5 most similar protocols
To overexpress circ-LRIG3, pLC5-ciR-GFP (#GS0108) vector containing one paired reverse complementary sequence (RCS) provided by Geneseed (Guangzhou, China) was used, in which the full-length of circ-LRIG3 (chr12: 59277301-59308117) was inserted into pLC5-ciR vector between EcoRI and BamHI restriction sites, followed by packaging into the lentivirus and infection into HepG2 cells. The stable circ-LRIG3-overexpressing cells were selected by 0.8μg/mL puromycin. To knock down circ-LRIG3, two antisense oligonucleotide (ASO) against circ-LRIG3 junction site were designed and synthesized by RiboBio (Guangzhou, China). Then, the ASO was transfected into SMMC-7721 cells using riboFECT™ CP transfection solution (RiboBio) based on manufacturer's protocol.
Sun S., Gao J., Zhou S., Li Y., Wang Y., Li J., Li J., Liu B., Zhang B., Han S., Ding H., & Li X. (2020). A novel circular RNA circ-LRIG3 facilitates the malignant progression of hepatocellular carcinoma by modulating the EZH2/STAT3 signaling.
+ Open protocol
+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required

Sign up now

Revolutionizing how scientists
search and build protocols!