Peripheral blood mononuclear cells (PBMCs) were obtained from three HCC patients. Written informed consents were signed from each patient in accordance with the Declaration of Helsinki. PBMCs were isolated using Human Lymphocyte separation medium Ficoll (GE lifesciences) and cultured in RPMI‐1640 medium containing 10% fetal bovine serum (FBS; Gibco; Thermo Fisher Scientific). 100 U/mL penicillin and 100 ng/mL streptomycin were added to the medium.
Human lymphocyte separation medium ficoll
Manufactured by GE Healthcare
Sourced in United States
Ficoll is a lab equipment product used for the separation and isolation of human lymphocytes. It is a dense, sterile, and endotoxin-tested liquid medium that allows the separation of mononuclear cells from whole blood through centrifugation.
Automatically generated - may contain errors
Lab products found in correlation
Phosphate buffered saline (pbs), by Solarbio (1 mentions)
Pomalidomide, by Merck Group (1 mentions)
Gm csf, by Novoprotein (1 mentions)
Interleukin 4 (il 4), by Novoprotein (1 mentions)
Human ab serum, by Merck Group (1 mentions)
Rpmi 1640 medium, by Thermo Fisher Scientific (1 mentions)
Dimethyl sulfoxide (dmso), by Solarbio (1 mentions)
2 protocols using human lymphocyte separation medium ficoll
1
PBMC Isolation from HCC Patients
2
Generation of Human Dendritic Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Pomalidomide and human AB serum were purchased from Sigma, United States. RPMI 1,640 medium was purchased from Gibco, United States. GM-CSF and IL-4 were purchased from Novoprotein, China. Penicillin-streptomycin solution was purchased from Hyclone, United States. Human Lymphocyte separation medium (Ficoll) was purchased from GE Lifesciences, United States. 1×PBS and DMSO were purchased from Solarbio, China. FITC anti-human CD80 mAb, PE anti-human CD86 mAb, APC anti-human CD40 mAb and Pacific Blue anti-human HLA-DR mAb were all purchased from Biolegend, China.
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