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Anti ptrka y490

Manufactured by Cell Signaling Technology
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Anti-pTrkA (Y490) is a rabbit monoclonal antibody that recognizes the phosphorylated tyrosine 490 residue of the TrkA protein. This antibody is designed for the detection of the activated form of the TrkA receptor by techniques such as Western blotting and immunohistochemistry.

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Lab products found in correlation

Anti akt, by Cell Signaling Technology (1 mentions) Anti p erk1 2 t202 y204, by Cell Signaling Technology (1 mentions) Anti pakt t308, by Cell Signaling Technology (1 mentions) Anti erk1 2, by Cell Signaling Technology (1 mentions) Anti gapdh, by Cell Signaling Technology (1 mentions) Mini protean system, by Bio-Rad (1 mentions) Picrotoxin, by Merck Group (1 mentions) Strychnine, by Merck Group (1 mentions) Anti trk, by Santa Cruz Biotechnology (1 mentions) Anti plcγ, by Cell Signaling Technology (1 mentions) Anti pakt s473, by Cell Signaling Technology (1 mentions) Anti akt 11e7, by Cell Signaling Technology (1 mentions) Ethanol, by Merck Group (1 mentions) Tetrodotoxin, by Alomone (1 mentions)

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PTrkA (5 citations)

2 protocols using anti ptrka y490

1

Immunoblotting Protocol for Protein Analysis

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Immunoblotting experiments were carried out as previously described.54 (link) Briefly, after desired treatment, cultured cells were lysed in PB/Triton buffer (7.74 mM Na2HPO4, 2.26 mM NaH2PO4, 5 mM EDTA, 5 mM EGTA, 100 mM NaCl, 10 mM Na4P2O7, 50 mM NaF, 1 mM Na3VO4, 1% Triton X-100), and clarified lysates were mixed with Laemmli sample buffer (Bio-Rad 1610747) and β-mercaptoethanol and boiled for 10 min. Lysed samples were subjected to electrophoresis using Bio-Rad’s Mini-PROTEAN system (1658026FC). After separation, protein was transferred to a polyvinylidene fluoride membrane (Bio-Rad 1704273), followed by standard blotting procedure. Primary antibodies were obtained from Cell Signaling Technology: anti-pTrkA (Y490) (CST 9141S), anti-pAKT (T308) (CST 9275), anti-AKT (CST 9272), anti-pERK1/2 (T202+Y204) (CST 9101), anti-ERK1/2 (CST 9102), and anti-GAPDH (CST 2118). HRP-conjugated secondary antibody (CST 7074) was used for protein band detection. Protein bands were visualized by chemiluminescence (Bio Rad 1705060).
Duan L., Hope J.M., Guo S., Ong Q., François A., Kaplan L., Scherrer G, & Cui B. (2018). Optical Activation of TrkA Signaling. ACS synthetic biology, 7(7), 1685-1693.
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2

Molecular signaling mechanisms in neurons

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Reagents: picrotoxin and strychnine were purchased from Sigma (St. Louis, MO, USA) and were dissolved in ethanol (96%, Sigma) and water, respectively. Tetrodotoxin (TTX; Alomone Labs, Jerusalem, Israel) was dissolved in water.
Primary Antibodies: anti-pTrkAY490 (Cell Signalling, Danvers, MA, USA, 9141), anti-Trk (Santa Cruz, Santa Cruz, CA, USA, sc7268), anti-pShcY317 (Cell Signalling, 2434), anti-ShcC (Cell Signalling, 2431), anti-pPLC-γ Y783 (Cell Signalling, 2821), anti PLC-γ (Cell Signalling, 14008), anti-pMAPKY202/Y204 (Cell Signalling, 9101), anti-MAPK (Cell Signalling, 9102), anti-pPI3KY458/Y199 (Cell Signalling, 4228), anti-pAKTS473 (Cell Signalling, 4051), anti-AKT (11E7) (Cell Signalling, 4685), anti-pCREBS133 (Millipore, Temecula, CA, USA, 06-519), anti-BDNF (Millipore, MABN110), anti-c-Fos (Cell Signalling, Rb Mab 2250S), anti-ChAT (Millipore, AB144P), and anti-NeuN (ABCAM, city, state abbrev if USA, country ab177487), anti-MAP2 (Cell Signalling, 4542; Millipore, MAB3418). Secondary antibodies: anti mouse-HRP and anti rabbit-HRP (PerkinElmer, Waltham, MA, USA); Donkey anti mouse-Alexa546, donkey anti rabbit-Alexa488 (Life Technologies, Carlsbad, CA, USA).
Triaca V., Fico E., Sposato V., Caioli S., Ciotti M.T., Zona C., Mercanti D., La Mendola D., Satriano C., Rizzarelli E., Tirassa P, & Calissano P. (2020). hNGF Peptides Elicit the NGF-TrkA Signalling Pathway in Cholinergic Neurons and Retain Full Neurotrophic Activity in the DRG Assay. Biomolecules, 10(2), 216.
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