Sb218078

Manufactured by Merck Group

Sourced in Germany

SB218078 is a laboratory instrument designed for scientific research applications. It is a highly precise and versatile piece of equipment that can be used for a variety of tasks in the field of analytical chemistry, life sciences, and materials science. The core function of SB218078 is to provide accurate and reliable measurements and data collection, enabling researchers to conduct their experiments with confidence and precision.

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Lab products found in correlation

2 protocols using sb218078

Antibody-based Detection of DNA Damage Markers

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Primary antibodies used were rabbit antibodies against ATRX (sc-15408, Santa Cruz), H3.3 (09–838, Millipore Merck), phosphorylated H3.3S31 (ab2889, Abcam; Active Motif, 39637; H3.3S31ph) (Abcam; Active Motif), CHK1 Ser317 (12302, Cell Signaling Technology), CHK2 Thr68 (abe343, Cell Signaling Technology), CHK1 (NBP1–19210, Novus Biologicals) and CHK2 (05–649, Millipore Merck); mouse monoclonal antibodies against γH2AX Ser139 (05–636, Millipore Merck), and myc tag (05–724, Millipore Merck), tubulin (Roche), and human anti centromere CREST serum (34 (link)). CHK1 inhibitor UCN-01 (Millipore Merck) and SB218078 (Millipore Merck) were used at a concentration of 1 μM and 5 μM, respectively (36 (link)). CHK2 inhibitor II hydrate (Sigma Aldrich) was used at a final concentration of 250 μM.

Chang F.T., Chan F.L., R. McGhie J.D., Udugama M., Mayne L., Collas P., Mann J.R, & Wong L.H. (2015). CHK1-driven histone H3.3 serine 31 phosphorylation is important for chromatin maintenance and cell survival in human ALT cancer cells. Nucleic Acids Research, 43(5), 2603-2614.

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Phosphorylation of Retinoblastoma Protein

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RPMI1640 medium, DMEM, TPA, all-trans retinoic acid (ATRA), hemin, etoposide and GW8510 were purchased from Sigma (St. Louis, MO, USA). Chk2 inhibitor II, SB218078 and PD98059 were from Merck (Darmstadt, Germany). λ protein phosphatase was obtained from New England Biolabs (Ipswich, MA, USA). Anti-pRB monoclonal antibody (G3–245), anti-underphosphorylated (under-P) pRB monoclonal antibody (G99–549) and anti-Hsp90 monoclonal antibody (68) were from BD Biosciences (Franklin Lakes, NJ, USA). Anti-Phospho-Chk2 (Thr68) polyclonal antibody and anti-Myc monoclonal antibody (9B11) were from Cell Signaling Technology (Danvers, MA, USA). Anti-Lamin A/C monoclonal antibody (636), anti-Chk2 polyclonal antibody (H-300) and anti-E2F-1 polyclonal antibody (C-20) were from Santa Cruz (Santa Cruz, CA, USA). Phospho-specific pRB monoclonal (pThr356, pSer612 and pSer807) and polyclonal (pThr373, pSer608, pSer780, pSer795, pSer811, pThr821 and pThr826) antibodies were previously described [16] (link)–[21] (link).

Hattori T., Uchida C., Takahashi H., Yamamoto N., Naito M, & Taya Y. (2014). Distinct and Site-Specific Phosphorylation of the Retinoblastoma Protein at Serine 612 in Differentiated Cells. PLoS ONE, 9(1), e86709.

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