L6-GLUT4myc cells, a L6 skeletal muscle cell line (Kerafast, Inc., Boston, MA, USA), were harvested in α-MEM supplemented with a 10% FBS and 1% penicillin-streptomycin solution according to a previous study [12 (link)]. As prolonged exposure to low concentrations of EtBr inhibits the replication of mtDNA selectively, a L6-GLUT4myc cell line depleted of mtDNA was prepared by incubating the cells with EtBr (0.2 µg/ml) and uridine (50 µg/ml) for 3 weeks. The reversal of mtDNA depletion was achieved within 7 days by the removal of EtBr and uridine. The control cells were cultured for the same time period under normal harvesting conditions.
L6 glut4myc cells
Manufactured by Kerafast
Sourced in United States
L6-GLUT4myc cells are a cell line derived from rat skeletal muscle that stably expresses the GLUT4 glucose transporter with a c-Myc tag. These cells can be used to study glucose uptake and the trafficking of GLUT4 in response to insulin or other stimuli.
Automatically generated - may contain errors
Lab products found in correlation
3 4 5 dimethylthiazol 2 yl 2 5 diphenyltetrazolium bromide mtt reagent, by Merck Group (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Methoxyamine hydrochloride, by Merck Group (1 mentions)
Pyridine, by Merck Group (1 mentions)
N methyl n trimethylsilyl trifluoroacetamide mstfa, by Merck Group (1 mentions)
Horseradish peroxidase hrp conjugated goat anti rabbit antibodies, by Promega (1 mentions)
Polyclonal anti myc a 14, by Merck Group (1 mentions)
2 protocols using l6 glut4myc cells
1
Generating mtDNA-depleted L6-GLUT4myc cell line
2
L6-GLUT4myc Cell Line Protocol
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Materials: The rat L6 muscle cell line, stably expressing myc-tagged GLUT4 (L6-GLUT4myc cells), was obtained from Kerafast (Boston, MA, USA). Fetal bovine serum, modified Eagle’s medium (α-MEM), standard culture medium, and all other tissue culture reagents were purchased from Biological Industries (Beit Haemek, Israel). Horseradish peroxidase (HRP)-conjugated goat anti-rabbit antibodies were obtained from Promega (Madison, WI, USA). Polyclonal anti-myc (A-14); the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) reagent; methoxyamine hydrochloride; pyridine; N-methyl-N-(trimethylsilyl)-trifluoroacetamide (MSTFA); and other standard chemicals were purchased from Sigma Aldrich (St. Louis, MO, USA).
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