The analysis was performed using an Agilent GC–MSD system (GC/MSD 6890N/5975) equipped with a HP-88 Agilent capillary column (60 m × 0.25 mm; 0.20 μm film thickness), MSD ChemStation ver. E.02.02.1431 software (Agilent Technologies, Santa Clara, CA, USA), and a split–splitless injector. The oven temperature was programmed from 110 °C to 190 °C with 8 °C/min, held for 2 min at 110 °C and 13 min at 190 °C. The temperature of the injector was 250 °C. The injection volume was 1 μL (split ratio 150:1; split flow 180 mL/min). Helium was used as a carrier gas at a flow rate of 1.2 mL/min. A quadrupole mass spectrometer with electron ionization (EI) at 70 eV and with a full scan type acquisition mode (50 m/z to 500 m/z) was used as a detector connected with the GC. The temperature of the MS source and the MS quadrupole was set at 230 °C and 150 °C, respectively. Identification of the constituents was based on a comparison of their mass spectra with the mass spectra library NIST resources and retention times with standards.
Gc msd 6890n 5975
Manufactured by Agilent Technologies
The GC/MSD 6890N/5975 is a gas chromatography-mass spectrometry (GC/MS) system manufactured by Agilent Technologies. It is designed to separate and analyze complex mixtures of chemical compounds. The GC component is used to separate the individual components of the mixture, while the MSD (mass selective detector) component is used to identify and quantify those components based on their unique mass-to-charge ratios.
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2 protocols using gc msd 6890n 5975
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GC-MS Analysis of Compound Identification
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GC-MSD Analysis of Organic Compounds
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The analysis was performed using an Agilent GC-MSD system (GC/MSD 6890N/5975) equipped with a HP-88 Agilent capillary column (60 m × 0.25 mm; 0.20 μm film thickness), MSD ChemStation ver. E.02.02.1431 software (Agilent Technologies, Santa Clara, CA, United States), and a split–splitless injector. The oven temperature was programmed from 110 °C to 190 °C with 8 °C/min, hold for 2 min at 110 °C and 13 min at 190 °C. The temperature of the injector was 250 °C. The injection volume was 1 μL (split ratio 150:1; split flow 180 mL/min). Helium was used as a carrier gas at a flow rate of 1.2 mL/min. A quadrupole mass spectrometer with electron ionization (EI) at 70 eV and with a full scan type acquisition mode (50 m/z to 500 m/z) was used as a detector connected with the GC. The temperature of the MS source and the MS quadrupole was set to 230 °C and 150 °C, respectively. The identification of the constituents was based on a comparison of their mass spectra with the mass spectra library NIST resources and retention times with standards. Each sample was measured in three replicates.
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