After the kidneys were collected from the mice, an equivalent of protein was resolved on dodecyl sulfate (SDS)-polyacrylamide gels and transferred to polyvinylidene difluoride (PVDF) membranes. The antibodies used are shown below: AGEs and RAGE antibodies were from Abcam, UK; p-IKKα/β, IKKβ, IKKα, p-IκBα, IκBα and NF-κB p65 antibodies were from Cell Signaling Technology, USA; p-NF-κB p65 antibody was from Santa Cruz Biotechnology, Glostrup, Denmark and GAPDH antibody was from Beijing Zhong Shan Golden Bridge Biotechnology Co., Ltd., China, with a dilution of 1:1000. Horseradish peroxidase-labeled secondary antibody was from Beijing Zhong Shan Golden Bridge Biotechnology Co., Ltd., with a dilution of 1:10,000. The membranes were developed with enhanced chemiluminescence (Thermo Scientific, USA) and visualized using a digital imaging system (BIO-RAD Laboratories, Inc., USA).
P nf κb p65 antibody
Manufactured by Santa Cruz Biotechnology
Sourced in United States
The P-NF-κB p65 antibody is a tool used to detect and quantify the phosphorylated form of the NF-κB p65 subunit. NF-κB p65 is a key transcription factor involved in various cellular processes, including immune response, inflammation, and cell survival. This antibody can be used in techniques such as Western blotting, immunohistochemistry, and ELISA to study the activation and regulation of the NF-κB signaling pathway.
Automatically generated - may contain errors
Lab products found in correlation
Digital imaging system, by Bio-Rad (1 mentions)
Enhanced chemiluminescence, by Thermo Fisher Scientific (1 mentions)
P iκbα, by Cell Signaling Technology (1 mentions)
P ikkα β, by Abcam (1 mentions)
Horseradish peroxidase labeled secondary antibody, by Zhongshan Golden Bridge Biotechnology (1 mentions)
Gapdh antibody, by Zhongshan Golden Bridge Biotechnology (1 mentions)
Nf κb p65, by Cell Signaling Technology (1 mentions)
Protein extraction kit, by Thermo Fisher Scientific (1 mentions)
P jnk antibody, by Santa Cruz Biotechnology (1 mentions)
Iκbα antibody, by Santa Cruz Biotechnology (1 mentions)
β actin, by Santa Cruz Biotechnology (1 mentions)
2 protocols using p nf κb p65 antibody
1
Protein Expression Analysis in Murine Kidneys
2
Protein Expression Analysis via Western Blot
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Total proteins were extracted using a protein extraction kit (Invitrogen, Carlsbad, USA), and Western blotting was performed according to the manufacturer’s instructions. Antibody concentrations were applied as follows: p-GSK-3β antibody (1:1000), p-JNK antibody (1:1000), p-NF-κB p65 antibody (1:1000), IκBα antibody (1:1000) and β-actin (1:1000, Santa Cruz, USA). The results are expressed relative to the control (control, set as 1.0).
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